Difference between revisions of "Team:Paris Saclay/Notebook/June/21"
(→Heat-shock competent cells preparation) |
(→Transformation (cf. protocole)) |
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50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. | 50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. | ||
| − | Petri | + | Petri dishes were prepared with selective medium containing LB, agar and 30µg/mL chloramphenicol. |
| − | Each transformation condition was | + | Each transformation condition was spread on Petri dishes in duplicate and incubated overnight at 37°C. |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 08:26, 12 July 2016
