Difference between revisions of "Team:Paris Saclay/Notebook/June/22"
(→Transformation) |
(→Competent cells) |
||
| Line 3: | Line 3: | ||
==Lab work== | ==Lab work== | ||
| − | ==== | + | ====Heat-shock competent cells (cf. [[Team:Paris_Saclay/Experiments#HeatShockCompetent|protocole]]==== |
''By Marion'' | ''By Marion'' | ||
At 10am OD was 0.3. At 1pm OD was 0.46. At 3pm OD was 0.62. | At 10am OD was 0.3. At 1pm OD was 0.46. At 3pm OD was 0.62. | ||
| − | The solution was put on ice 10min (stop the OD). After, it was centrifuged 10min at 4°C at 3000rpm. The pellet was resuspended in 80mL ice-cold TB and incubated on ice for 10min. The solution was centrifuged again at 4°C at 3000rpm. The pellet was resuspended in 20mL ice-cold TB and DMSO was added at 7% of the final concentration (1.58mL of DMSO for 21mL of TB+cells). The solution was incubated on ice for 10min and then distributed in tubes, frozen in liquid nitrogen and stored at -70°C. | + | The solution was put on ice 10min (stop the OD). After, it was centrifuged 10min at 4°C at 3000rpm. The pellet was resuspended in 80mL ice-cold TB and incubated on ice for 10min. The solution was centrifuged again at 4°C at 3000rpm. The pellet was resuspended in 20mL ice-cold TB and DMSO was added at 7% of the final concentration (1.58mL of DMSO for 21mL of TB+cells). The solution was incubated on ice for 10min and then distributed in tubes, frozen in liquid nitrogen and stored at -70°C. |
| − | + | ||
===Interlab study=== | ===Interlab study=== | ||
Revision as of 08:27, 12 July 2016
