Thursday 23^rd June

Lab work

Competent cells

By Caroline and Lea

The cells competency was checked by transforming cells as usual with control plasmids (positive control: pSB1C3+blue; negative control).

Interlab study

Transformation results

By Caroline, Lea and Marion

Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely.

Transformed cell cultures

By Lea

All colonies that were obtained after transformation were put into culture. One colony was taken into 3.5mL LB medium + Cm at 30µg/mL.