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| + | #attributions article .p { |
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| <div class="article"> | | <div class="article"> |
| <div class="p"> | | <div class="p"> |
− | Xiao Liu, Xinhao Song, Liangti Dai: | + | Xinhao Song, Xiao Liu, Liangti Dai, Yuxiu Xiong: |
| </div> | | </div> |
| <div class="p"> | | <div class="p"> |
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| </div> | | </div> |
| <div class="p"> | | <div class="p"> |
− | Mathematical Models; Wiki Construction; | + | Mathematical Modeling; Wiki Construction; |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div id="details"> | | <div id="details"> |
− | <div class="h1">Attributions in details</div> | + | <div class="h1">Attributions in detail</div> |
| <article> | | <article> |
| <div class="h2">Project: the idea</div> | | <div class="h2">Project: the idea</div> |
− | <div class="p">We came to the "AI-2 Controller" project after many brainstorming meetings including all the team. It would be impossible to define authors here: we all contributed to the design of the project.</div> | + | <div class="p">We came to the "AI-2 Controller" project after many brainstorming meetings including all the team members. It would be reasonable to attribute the idea of the project to all the team members because we all contributed to the design of the project..</div> |
| </article> | | </article> |
| <article> | | <article> |
| <div class="h2">AI-2 Controller Construction:</div> | | <div class="h2">AI-2 Controller Construction:</div> |
| <div class="p"> | | <div class="p"> |
− | All the experiments including AI-2 controller gene circuits construction, AI-2 response device construction and AI-2 controller biosafety construction was designed and conducted by Fankang Meng, Xinyu Ma, Yujie Zhao, Jingzhe Dong. | + | All the experiments including AI-2 controller gene circuits construction, AI-2 response device construction and AI-2 controller biosafety construction were designed and conducted by Fankang Meng, Xinyu Ma, Yujie Zhao and Jingzhe Dong. |
| </div> | | </div> |
| <div class="p"> | | <div class="p"> |
− | This part of construction includes using homologous recombination technology to construct pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK, AI-2 Response Device A and B. What's more, these four people also take advantage of mf-lon protease to create safer engineered bacteria. | + | This part of construction includes the construction of pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK, AI-2 Response Device A and B using homologous recombination method. What<U+00A1><U+00AF>s more, the four people listed before also took advantage of <i>mf</i>-lon protease to create safer engineered bacteria. |
| </div> | | </div> |
| </article> | | </article> |
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| <div class="h2">AI-2 Controller Characterization:</div> | | <div class="h2">AI-2 Controller Characterization:</div> |
| <div class="p"> | | <div class="p"> |
− | All the experiments in AI-2 controller characterization was designed and conducted by Fankang Meng, Xiao Liu and Xinhao Song. | + | All the experiments in AI-2 controller characterization were designed and conducted by Fankang Meng, Xiao Liu and Xinhao Song. |
| + | This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK. |
| + | </div> |
| + | <div class="p"> |
| This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK. | | This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK. |
| </div> | | </div> |
| </article> | | </article> |
| <article> | | <article> |
− | <div class="h2">Model:</div> | + | <div class="h2">Moedling:</div> |
| <div class="p"> | | <div class="p"> |
− | This part of work was designed and conducted by Zhuoer Dong, Meng Fankang. | + | This part of work was designed and conducted by Zhuoer Dong, Fankang Meng. |
| </div> | | </div> |
| </article> | | </article> |
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| <div class="h2">Parts Construction:</div> | | <div class="h2">Parts Construction:</div> |
| <div class="p"> | | <div class="p"> |
− | Xinhao Song, Liu Xiao, Liangti Dai and Yuxiu Xiong performed all the work for parts standardization. | + | Xinhao Song, Xiao Liu, Liangti Dai and Yuxiu Xiong performed all the work of parts standardization. |
| </div> | | </div> |
| </article> | | </article> |
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| <div class="h2">Interlab Study:</div> | | <div class="h2">Interlab Study:</div> |
| <div class="p"> | | <div class="p"> |
− | Liu Xiao, Xinhao Song, Liangti Dai and Yuxiu Xiong performed all the work for interlab study. | + | Xinhao Song, Xiao Liu, Liangti Dai and Yuxiu Xiong performed all the work of interlab study. |
| </div> | | </div> |
| </article> | | </article> |
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| <div class="h2">Human Practices.</div> | | <div class="h2">Human Practices.</div> |
| <div class="p"> | | <div class="p"> |
− | Xinglin Zhao, Liangti Dai, Zhuoer Dong and Ruilin Tian organized the "Synfuture" Exposition. Also they organized the iGEM Meeting with iGEM team TJUSLS_China. What's more, they also interviewed many famous scholars to get suggestions which greatly improved our project. | + | Xinglin Zhao, Liangti Dai, Zhuoer Dong and Ruilin Tian organized the "Synfuture" Exposition. Also they organized the iGEM Meeting with iGEM team TJUSLS_China. What' s more, they also interviewed many famous scholars to gather suggestions which greatly improved our project. |
| </div> | | </div> |
| </article> | | </article> |
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| <div class="h2">Wiki Construction</div> | | <div class="h2">Wiki Construction</div> |
| <div class="p"> | | <div class="p"> |
− | This part of work was designed and conducted by Zhuoer Dong, Xianglin zhao. | + | Zhuoer Dong conducted most of the work for wiki construction, including page design and implementation. He edited web pages, uploaded resources (images, fonts etc.) onto iGEM server and, moreover, fixed numerous bugs. Special acknowledgement must be attributed to dedicated chief editor—Xinhao Song. He proofread all the material several times. Other team members including Fankang Meng, Xinyu Ma, Xiao Liu, Liangti Dai, and Xinglin Zhao kindly provided contents for our wiki. |
| </div> | | </div> |
| </article> | | </article> |
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| <div class="h1">Supervision</div> | | <div class="h1">Supervision</div> |
| <div class="p"> | | <div class="p"> |
− | <b>THE INSTRUCTORS</b> were closely involved keeping the message clear and the team on track to reaching the goals. | + | <b>THE INSTRUCTORS</b> were closely involved to keep the instructions in time and the team on track to reach the goals. |
| </div> | | </div> |
| <div class="p"> | | <div class="p"> |
− | <b>Dr. Yufen Quan</b> as our instructor keeps the great mood in the lab and his accurate questions leads everyone to the result without any need for explanation. | + | <b>Yufen Quan</b> kept a relaxed atmosphere in the lab and her instructions were so clear that we could easily obtain the expected results without the need for further explanations. |
| </div> | | </div> |
| <div class="p"> | | <div class="p"> |
− | <b>Dr. Yulei Dang</b> provided tremendous organizational support for our team throughout the duration. | + | <b>Yulei Dang</b> provided tremendous organizational support for our team throughout the whole process. |
| </div> | | </div> |
| <div class="p"> | | <div class="p"> |
− | <b>Dr. Jiwei Mao & Dr. Xiang Wang</b> gave us helpful advice on AI-2 Controller Protein level experiments. | + | <b>Jiwei Mao & Xiangxiang Wang</b> gave us helpful advice on AI-2 Controller Protein level experiments. |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="h1">Acknowledgements</div> | | <div class="h1">Acknowledgements</div> |
| <div class="p"> | | <div class="p"> |
− | We would like to thank the <U+FFFD><U+FFFD>The Ministry Key Laboratory of Molecular Microbiology and Technology<U+FFFD><U+FFFD> and School of Boling for helping us construct the new iGEM laboratory. Namely we would also like to thank Prof. Weihui Wu, Dr. Honggang Wang for letting us use the microplate and fluorescence microscope when we characterized the AI-2 Responser. We would like to also thank Prof. Lingyi Chen and Prof. Zhihui Cheng for sharing their experience with circuits construction and for giving us helpful advice on how to improve our experiments to get better data. | + | We would like to thank the 'The Ministry Key Laboratory of Molecular Microbiology and Technology' and Boling College for helping us establish the new iGEM laboratory. We would like to thank Prof. Weihui Wu and Prof. Honggang Wang for letting us use the microplate reader and fluorescence microscope when we characterized the AI-2 Responser. We would like to thank Prof. Lingyi Chen and Prof. Zhihui Cheng for sharing their experience on circuits construction and for giving us helpful advice on how to improve our experiments to obtain better data. |
| </div> | | </div> |
| <div class="p"> | | <div class="p"> |
− | Thanks to all our colleagues at the College of Life Sciences for their support during our research. Special thanks belongs to Prof. Cunjiang Song for opening up his Introduction to biotechnology course for an hour allowing us to present our project to freshmen in the College of Life sciences. | + | We would like to thank all our colleagues at the College of Life Sciences for their support during our project. Particularly, we would like to thank Prof. Cunjiang Song for kindly allowing us to present our project to freshman students of College of Life Science in a course named "Introduction to Biotechnology" instructed by him. |
| </div> | | </div> |
| <div class="p"> | | <div class="p"> |
− | Finally, we want to thank teams listed below for helping us improve our project on data, model, human practice, etc. | + | Finally, we want to thank teams listed below for helping us improve our project on data process and analysis, modelling, human practice, etc. |
| </div> | | </div> |
| <div class="p"> | | <div class="p"> |
| <ul style="list-style:disc"> | | <ul style="list-style:disc"> |
| <li>iGEM Team NAU_China;</li> | | <li>iGEM Team NAU_China;</li> |
− | <li>iGEM Team SLSTJU;</li> | + | <li>iGEM Team TJUSLS_China;</li> |
| <li>iGEM Team Tianjin;</li> | | <li>iGEM Team Tianjin;</li> |
| </ul> | | </ul> |
Attributions in AI-2 Controller Construction
Fankang Meng, Xinyu Ma, Yujie Zhao, Jingzhe Dong:
AI-2 Controller Gene Circuits Construction;
AI-2 Response Device Construction;
AI-2 Controller Biosafety Construction;
Xinhao Song, Xiao Liu, Liangti Dai, Yuxiu Xiong:
AI-2 Controller Characterization;
Parts Construction;
Interlab Study;
Zhuoer Dong:
Mathematical Modeling; Wiki Construction;
Xianglin Zhao:
Human Practice; Art Design;
Attributions in detail
Project: the idea
We came to the "AI-2 Controller" project after many brainstorming meetings including all the team members. It would be reasonable to attribute the idea of the project to all the team members because we all contributed to the design of the project..
AI-2 Controller Construction:
All the experiments including AI-2 controller gene circuits construction, AI-2 response device construction and AI-2 controller biosafety construction were designed and conducted by Fankang Meng, Xinyu Ma, Yujie Zhao and Jingzhe Dong.
This part of construction includes the construction of pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK, AI-2 Response Device A and B using homologous recombination method. Whats more, the four people listed before also took advantage of mf-lon protease to create safer engineered bacteria.
AI-2 Controller Characterization:
All the experiments in AI-2 controller characterization were designed and conducted by Fankang Meng, Xiao Liu and Xinhao Song.
This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK.
This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK.
Moedling:
This part of work was designed and conducted by Zhuoer Dong, Fankang Meng.
Parts Construction:
Xinhao Song, Xiao Liu, Liangti Dai and Yuxiu Xiong performed all the work of parts standardization.
Interlab Study:
Xinhao Song, Xiao Liu, Liangti Dai and Yuxiu Xiong performed all the work of interlab study.
Human Practices.
Xinglin Zhao, Liangti Dai, Zhuoer Dong and Ruilin Tian organized the "Synfuture" Exposition. Also they organized the iGEM Meeting with iGEM team TJUSLS_China. What' s more, they also interviewed many famous scholars to gather suggestions which greatly improved our project.
Wiki Construction
Zhuoer Dong conducted most of the work for wiki construction, including page design and implementation. He edited web pages, uploaded resources (images, fonts etc.) onto iGEM server and, moreover, fixed numerous bugs. Special acknowledgement must be attributed to dedicated chief editor—Xinhao Song. He proofread all the material several times. Other team members including Fankang Meng, Xinyu Ma, Xiao Liu, Liangti Dai, and Xinglin Zhao kindly provided contents for our wiki.
Supervision
THE INSTRUCTORS were closely involved to keep the instructions in time and the team on track to reach the goals.
Yufen Quan kept a relaxed atmosphere in the lab and her instructions were so clear that we could easily obtain the expected results without the need for further explanations.
Yulei Dang provided tremendous organizational support for our team throughout the whole process.
Jiwei Mao & Xiangxiang Wang gave us helpful advice on AI-2 Controller Protein level experiments.
Acknowledgements
We would like to thank the 'The Ministry Key Laboratory of Molecular Microbiology and Technology' and Boling College for helping us establish the new iGEM laboratory. We would like to thank Prof. Weihui Wu and Prof. Honggang Wang for letting us use the microplate reader and fluorescence microscope when we characterized the AI-2 Responser. We would like to thank Prof. Lingyi Chen and Prof. Zhihui Cheng for sharing their experience on circuits construction and for giving us helpful advice on how to improve our experiments to obtain better data.
We would like to thank all our colleagues at the College of Life Sciences for their support during our project. Particularly, we would like to thank Prof. Cunjiang Song for kindly allowing us to present our project to freshman students of College of Life Science in a course named "Introduction to Biotechnology" instructed by him.
Finally, we want to thank teams listed below for helping us improve our project on data process and analysis, modelling, human practice, etc.
- iGEM Team NAU_China;
- iGEM Team TJUSLS_China;
- iGEM Team Tianjin;