Difference between revisions of "Team:NKU China/Attributions"

 
(26 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{NKU_China}}
+
{{NKU_China/menubar}}
 
<html>
 
<html>
 +
<head>
 +
   
 +
    <!--separatrix-->
 +
    <style>
 +
        .main#attributions {
 +
            padding: 0 10vw;
 +
            padding-bottom:10vh;
 +
            font-size: 1.15rem;
 +
            line-height:1.25;
 +
        }
  
−
<script>
+
        .main div.h2 {
−
document.write('hello')
+
            color:cyan;
−
</script>
+
            text-align: left;
 +
            font-size:2rem;
 +
            padding-bottom:1rem;
 +
            font-family: Pacifico;
 +
        }
  
−
<div class="column full_size judges-will-not-evaluate">
+
        article {
−
<h3>★  ALERT! </h3>
+
            margin-bottom: 2.5rem;
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">Attributions bronze criterion</a>. </p>
+
        }
  
 +
        #attributions article .p {
 +
            margin:0.5rem;
 +
        }
  
 +
        #nutshell {
 +
            padding:1rem 0;
 +
        }
  
−
<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
+
        #nutshell .article {
−
</div>
+
            margin: 1rem 0.4rem;
 +
            line-height:1.6rem;
 +
        }
  
 +
        #nutshell .article div:first-child {
 +
            font-weight: bold;
 +
            color: cyan;
 +
            text-indent:0;
 +
        }
  
 +
        #nutshell img, #nutshell > div:last-child {
 +
            display: inline-block;
 +
            margin: 0;
 +
            padding: 0;
 +
            vertical-align: top;
 +
        }
  
 +
        #nutshell img {
 +
            margin-right:1rem;
 +
            vertical-align:middle;
 +
            height: 25rem;
 +
            position:relative;
 +
            z-index:3;
 +
        }
  
 +
        #supervision .p, #acknowledgements .p{
 +
            margin:1rem;
 +
        }
 +
    </style>
 +
    <script>
 +
        $(function () {
 +
            //particles();
 +
        })
 +
    </script>
 +
</head>
 +
<body id="dongzhuoer">
 +
    <div id="particles-js"></div>
 +
    <script>
 +
        particlesJS('particles-js',
 +
  {
 +
      "particles": {
 +
          "number": {
 +
              "value": 100,
 +
              "density": {
 +
                  "enable": true,
 +
                  "value_area": 800
 +
              }
 +
          },
 +
          "color": {
 +
              "value": "#0ff"
 +
          },
 +
          "shape": {
 +
              "type": "circle",
 +
              "stroke": {
 +
                  "width": 0,
 +
                  "color": "#000000"
 +
              },
 +
              "polygon": {
 +
                  "nb_sides": 5
 +
              },
 +
              "image": {
 +
                  "src": "img/github.svg",
 +
                  "width": 100,
 +
                  "height": 100
 +
              }
 +
          },
 +
          "opacity": {
 +
              "value": 0.4,
 +
              "random": false,
 +
              "anim": {
 +
                  "enable": false,
 +
                  "speed": 1,
 +
                  "opacity_min": 0,
 +
                  "sync": false
 +
              }
 +
          },
 +
          "size": {
 +
              "value": 5,
 +
              "random": true,
 +
              "anim": {
 +
                  "enable": false,
 +
                  "speed": 40,
 +
                  "size_min": 0,
 +
                  "sync": false
 +
              }
 +
          },
 +
          "line_linked": {
 +
              "enable": true,
 +
              "distance": 150,
 +
              "color": "#ff0",
 +
              "opacity": 0.4,
 +
              "width": 1
 +
          },
 +
          "move": {
 +
              "enable": true,
 +
              "speed": 6,
 +
              "direction": "none",
 +
              "random": false,
 +
              "straight": false,
 +
              "out_mode": "out",
 +
              "attract": {
 +
                  "enable": true,
 +
                  "rotateX": 600,
 +
                  "rotateY": 1200
 +
              }
 +
          }
 +
      },
 +
      "interactivity": {
 +
          "detect_on": "canvas",
 +
          "events": {
 +
              "onhover": {
 +
                  "enable": true,
 +
                  "mode": ["grab", "bubble"]
 +
              },
 +
              "onclick": {
 +
                  "enable": true,
 +
                  "mode": ["push", "repulse"]
 +
              },
 +
              "resize": true
 +
          },
 +
          "modes": {
 +
              "grab": {
 +
                  "distance": 100,
 +
                  "line_linked": {
 +
                      "opacity": 0.75
 +
                  }
 +
              },
 +
              "bubble": {
 +
                  "distance": 100,
 +
                  "size": 4,
 +
                  "duration": 2,
 +
                  "opacity": 8,
 +
                  "speed": 5
 +
              },
 +
              "repulse": {
 +
                  "distance": 200
 +
              },
 +
              "push": {
 +
                  "particles_nb": 4
 +
              },
 +
              "remove": {
 +
                  "particles_nb": 2
 +
              }
 +
          }
 +
      },
 +
      "retina_detect": true,
 +
      "config_demo": {
 +
          "hide_card": false,
 +
          "background_color": "#b61924",
 +
          "background_image": "",
 +
          "background_position": "50% 50%",
 +
          "background_repeat": "no-repeat",
 +
          "background_size": "cover"
 +
      }
 +
  }
  
−
 
+
);
−
 
+
    </script>
−
 
+
    <div class="main" id="attributions">
−
<div class="column full_size">
+
        <div id="nutshell">
−
 
+
            <div class="h1">
−
 
+
                Attributions in AI-2 Controller Construction
−
<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. </p>
+
            </div>
−
 
+
            <img src="https://static.igem.org/mediawiki/2016/0/0a/T--NKU_China--mascot.png">
−
</div>
+
            <div>
−
 
+
                <div class="article">
−
 
+
                    <div class="p">
−
<div class="clear"></div>
+
                        Fankang Meng, Xinyu Ma, Yujie Zhao, Jingzhe Dong:
−
 
+
                    </div>
−
 
+
                    <div class="p">
−
<div class="column half_size">
+
                        AI-2 Controller Gene Circuits Construction;
−
<h5> Why is this page needed? </h5>
+
                    </div>
−
<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
+
                    <div class="p">
−
<p>
+
                        AI-2 Response Device Construction;
−
For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
+
                    </div>
−
</div>
+
                    <div class="p">
−
 
+
                        AI-2 Controller Biosafety Construction;
−
 
+
                    </div>
−
<div class="column half_size">
+
                </div>
−
<h5> What should this page have?</h5>
+
                <div class="article">
−
 
+
                    <div class="p">
−
<ul>
+
                        Xinhao Song, Xiao Liu, Liangti Dai, Yuxiu Xiong:
−
<li>General Support</li>
+
                    </div>
−
<li>Project support and advice</li>
+
                    <div class="p">
−
<li>Fundraising help and advice</li>
+
                        AI-2 Controller Characterization;
−
<li>Lab support</li>
+
                    </div>
−
<li>Difficult technique support</li>
+
                    <div class="p">
−
<li>Project advisor support</li>
+
                        Parts Construction;
−
<li>Wiki support</li>
+
                    </div>
−
<li>Presentation coaching</li>
+
                    <div class="p">
−
<li>Human Practices support</li>
+
                        Interlab Study;
−
<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
+
                    </div>
−
</ul>
+
                </div>
−
</div>
+
                <div class="article">
−
 
+
                    <div class="p">
−
 
+
                        Zhuoer Dong:
−
<div class="clear"></div>
+
                    </div>
−
 
+
                    <div class="p">
−
<div class="column half_size">
+
                        Mathematical Modeling; Wiki Construction;
−
 
+
                    </div>
−
<div class="highlight">
+
                </div>
−
<h5> Can we base our project on a previous one? </h5>
+
                <div class="article">
−
<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
+
                    <div class="p">
−
</div>
+
                        Xianglin Zhao:
−
</div>
+
                    </div>
−
 
+
                    <div class="p">
−
 
+
                        Human Practice; Art Design;
−
<div class="column half_size">
+
                    </div>
−
 
+
                </div>
−
<h5>Inspiration</h5>
+
            </div>
−
<p>Take a look at what other teams have done:</p>
+
        </div>
−
<ul>
+
        <div id="details">
−
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
+
            <div class="h1">Attributions in detail</div>
−
<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
+
            <article>
−
<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
+
                <div class="h2">Project: the idea</div>
−
<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
+
                <div class="p">We came to the "AI-2 Controller" project after many brainstorming meetings including all the team members. It would be reasonable to attribute the idea of the project to all the team members because we all contributed to the design of the project..</div>
−
</ul>
+
            </article>
−
 
+
            <article>
−
</div>
+
                <div class="h2">AI-2 Controller Construction:</div>
−
 
+
                <div class="p">
−
<div class="clear"></div>
+
                    All the experiments including AI-2 controller gene circuits construction, AI-2 response device construction and AI-2 controller biosafety construction were designed and conducted by Fankang Meng, Xinyu Ma, Yujie Zhao and Jingzhe Dong.
−
 
+
                </div>
−
<div class="column half_size">
+
                <div class="p">
−
 
+
                    This part of construction includes the construction of pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK, AI-2 Response Device A and B using homologous recombination method. What<U+00A1><U+00AF>s more, the four people listed before also took advantage of <i>mf</i>-lon protease to create safer engineered bacteria.
−
<h5>Team training and Project start</h5>
+
                </div>
−
<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
+
            </article>
−
<ul>
+
            <article>
−
<li>Does your institution teach an iGEM or synthetic biology course?</li>
+
                <div class="h2">AI-2 Controller Characterization:</div>
−
<li>When did you start this course?</li>
+
                <div class="p">
−
<li>Are the syllabus and course materials freely available online?</li>
+
                    All the experiments in AI-2 controller characterization were designed and conducted by Fankang Meng, Xiao Liu and Xinhao Song.
−
<li>When did you start your brainstorming?</li>
+
                    This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK.
−
<li>When did you start in the lab?</li>
+
                </div>
−
<li>When did you start working on  your project?</li>
+
                <div class="p">
−
 
+
                    This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK.
−
</ul>
+
                </div>
−
 
+
            </article>
−
</div>
+
            <article>
−
 
+
                <div class="h2">Moedling:</div>
−
 
+
                <div class="p">
−
 
+
                    This part of work was designed and conducted by Zhuoer Dong, Fankang Meng.
−
 
+
                </div>
−
</div>
+
            </article>
 +
            <article>
 +
                <div class="h2">Parts Construction:</div>
 +
                <div class="p">
 +
                    Xinhao Song, Xiao Liu, Liangti Dai and Yuxiu Xiong performed all the work of parts standardization.
 +
                </div>
 +
            </article>
 +
            <article>
 +
                <div class="h2">Interlab Study:</div>
 +
                <div class="p">
 +
                    Xinhao Song, Xiao Liu, Liangti Dai and Yuxiu Xiong performed all the work of interlab study.
 +
                </div>
 +
            </article>
 +
            <article>
 +
                <div class="h2">Human Practices.</div>
 +
                <div class="p">
 +
                    Xinglin Zhao, Liangti Dai, Zhuoer Dong and Ruilin Tian organized the "Synfuture" Exposition. Also they organized the iGEM Meeting with iGEM team TJUSLS_China. What' s more, they also interviewed many famous scholars to gather suggestions which greatly improved our project.
 +
                </div>
 +
            </article>
 +
            <article>
 +
                <div class="h2">Wiki Construction</div>
 +
                <div class="p">
 +
                    Zhuoer Dong conducted most of the work for wiki construction, including page design and implementation. He edited web pages, uploaded resources (images, fonts etc.) onto iGEM server and, moreover, fixed numerous bugs. Special acknowledgement must be attributed to dedicated chief editor&mdash;Xinhao Song. He proofread all the material several times. Other team members including Fankang Meng, Xinyu Ma, Xiao Liu, Liangti Dai, and Xinglin Zhao kindly provided contents for our wiki.
 +
                </div>
 +
            </article>
 +
        </div>
 +
        <div id="supervision">
 +
            <div class="h1">Supervision</div>
 +
            <div class="p">
 +
                <b>THE INSTRUCTORS</b> were closely involved to keep the instructions in time and the team on track to reach the goals.
 +
            </div>
 +
            <div class="p">
 +
                <b>Yufen Quan</b> kept a relaxed atmosphere in the lab and her instructions were so clear that we could easily obtain the expected results without the need for further explanations.
 +
            </div>
 +
            <div class="p">
 +
                <b>Yulei Dang</b> provided tremendous organizational support for our team throughout the whole process.
 +
            </div>
 +
            <div class="p">
 +
                <b>Jiwei Mao & Xiangxiang Wang</b> gave us helpful advice on AI-2 Controller Protein level experiments.
 +
            </div>
 +
        </div>
 +
        <div id="acknowledgements">
 +
            <div class="h1">Acknowledgements</div>
 +
            <div class="p">
 +
                We would like to thank the 'The Ministry Key Laboratory of Molecular Microbiology and Technology' and Boling College for helping us establish the new iGEM laboratory. We would like to thank Prof. Weihui Wu and Prof. Honggang Wang for letting us use the microplate reader and fluorescence microscope when we characterized the AI-2 Responser. We would like to thank Prof. Lingyi Chen and Prof. Zhihui Cheng for sharing their experience on circuits construction and for giving us helpful advice on how to improve our experiments to obtain better data.
 +
            </div>
 +
            <div class="p">
 +
                We would like to thank all our colleagues at the College of Life Sciences for their support during our project. Particularly, we would like to thank Prof. Cunjiang Song for kindly allowing us to present our project to freshman students of College of Life Science in a course named "Introduction to Biotechnology" instructed by him.
 +
            </div>
 +
            <div class="p">
 +
                Finally, we want to thank teams listed below for helping us improve our project on data process and analysis, modelling, human practice, etc.
 +
            </div>
 +
            <div class="p">
 +
                <ul style="list-style:disc">
 +
                    <li>iGEM Team NAU_China;</li>
 +
                    <li>iGEM Team TJUSLS_China;</li>
 +
                    <li>iGEM Team Tianjin;</li>
 +
                </ul>
 +
            </div>
 +
        </div>
 +
    </div>
 +
</body>
 
</html>
 
</html>
 +
{{NKU_China/footer}}

Latest revision as of 13:36, 19 October 2016

Attributions in AI-2 Controller Construction
Fankang Meng, Xinyu Ma, Yujie Zhao, Jingzhe Dong:
AI-2 Controller Gene Circuits Construction;
AI-2 Response Device Construction;
AI-2 Controller Biosafety Construction;
Xinhao Song, Xiao Liu, Liangti Dai, Yuxiu Xiong:
AI-2 Controller Characterization;
Parts Construction;
Interlab Study;
Zhuoer Dong:
Mathematical Modeling; Wiki Construction;
Xianglin Zhao:
Human Practice; Art Design;
Attributions in detail
Project: the idea
We came to the "AI-2 Controller" project after many brainstorming meetings including all the team members. It would be reasonable to attribute the idea of the project to all the team members because we all contributed to the design of the project..
AI-2 Controller Construction:
All the experiments including AI-2 controller gene circuits construction, AI-2 response device construction and AI-2 controller biosafety construction were designed and conducted by Fankang Meng, Xinyu Ma, Yujie Zhao and Jingzhe Dong.
This part of construction includes the construction of pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK, AI-2 Response Device A and B using homologous recombination method. Whats more, the four people listed before also took advantage of mf-lon protease to create safer engineered bacteria.
AI-2 Controller Characterization:
All the experiments in AI-2 controller characterization were designed and conducted by Fankang Meng, Xiao Liu and Xinhao Song. This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK.
This part of construction includes using qPCR, Western blot, microplate reader and HPLC to characterize pTrcHisB, pLuxS, pLuxS-Mtn, pLsrACDB, pLsrACDBFG, pLsrACDBK, pLsrACDBFGK.
Moedling:
This part of work was designed and conducted by Zhuoer Dong, Fankang Meng.
Parts Construction:
Xinhao Song, Xiao Liu, Liangti Dai and Yuxiu Xiong performed all the work of parts standardization.
Interlab Study:
Xinhao Song, Xiao Liu, Liangti Dai and Yuxiu Xiong performed all the work of interlab study.
Human Practices.
Xinglin Zhao, Liangti Dai, Zhuoer Dong and Ruilin Tian organized the "Synfuture" Exposition. Also they organized the iGEM Meeting with iGEM team TJUSLS_China. What' s more, they also interviewed many famous scholars to gather suggestions which greatly improved our project.
Wiki Construction
Zhuoer Dong conducted most of the work for wiki construction, including page design and implementation. He edited web pages, uploaded resources (images, fonts etc.) onto iGEM server and, moreover, fixed numerous bugs. Special acknowledgement must be attributed to dedicated chief editor—Xinhao Song. He proofread all the material several times. Other team members including Fankang Meng, Xinyu Ma, Xiao Liu, Liangti Dai, and Xinglin Zhao kindly provided contents for our wiki.
Supervision
THE INSTRUCTORS were closely involved to keep the instructions in time and the team on track to reach the goals.
Yufen Quan kept a relaxed atmosphere in the lab and her instructions were so clear that we could easily obtain the expected results without the need for further explanations.
Yulei Dang provided tremendous organizational support for our team throughout the whole process.
Jiwei Mao & Xiangxiang Wang gave us helpful advice on AI-2 Controller Protein level experiments.
Acknowledgements
We would like to thank the 'The Ministry Key Laboratory of Molecular Microbiology and Technology' and Boling College for helping us establish the new iGEM laboratory. We would like to thank Prof. Weihui Wu and Prof. Honggang Wang for letting us use the microplate reader and fluorescence microscope when we characterized the AI-2 Responser. We would like to thank Prof. Lingyi Chen and Prof. Zhihui Cheng for sharing their experience on circuits construction and for giving us helpful advice on how to improve our experiments to obtain better data.
We would like to thank all our colleagues at the College of Life Sciences for their support during our project. Particularly, we would like to thank Prof. Cunjiang Song for kindly allowing us to present our project to freshman students of College of Life Science in a course named "Introduction to Biotechnology" instructed by him.
Finally, we want to thank teams listed below for helping us improve our project on data process and analysis, modelling, human practice, etc.
  • iGEM Team NAU_China;
  • iGEM Team TJUSLS_China;
  • iGEM Team Tianjin;