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<div class="header-subtitle">Safety</div> | <div class="header-subtitle">Safety</div> | ||
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− | <p class="text" id="lab_safety">Before starting the | + | <p class="text" id="lab_safety">Before starting the wet lab part of the project all team members |
had to take part in a safety lecture held by the lab manager at the Systems and | had to take part in a safety lecture held by the lab manager at the Systems and | ||
− | synthetic biology department. During this lecture the | + | synthetic biology department. During this lecture the team learned how to handle |
chemicals and waste in the lab, we also had a lab tour where the safety equipment | chemicals and waste in the lab, we also had a lab tour where the safety equipment | ||
were introduced. During the lab tour all the responsible research engineers were | were introduced. During the lab tour all the responsible research engineers were | ||
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<h3 class="sidetitle" id="risk_declaration">Risk declaration</h3> | <h3 class="sidetitle" id="risk_declaration">Risk declaration</h3> | ||
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<li class="list"><a href="https://static.igem.org/mediawiki/2016/d/d0/T--Chalmers_Gothenburg--Riskdeclaration.pdf">Risk Declaration 2016</a></li> | <li class="list"><a href="https://static.igem.org/mediawiki/2016/d/d0/T--Chalmers_Gothenburg--Riskdeclaration.pdf">Risk Declaration 2016</a></li> | ||
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<li class="list"><a href="https://static.igem.org/mediawiki/2016/e/ee/T--Chalmers_Gothenburg--G418.pdf">G418 disulfate salt colution</a></li> | <li class="list"><a href="https://static.igem.org/mediawiki/2016/e/ee/T--Chalmers_Gothenburg--G418.pdf">G418 disulfate salt colution</a></li> | ||
<li class="list"><a href="https://static.igem.org/mediawiki/2016/1/1e/T--Chalmers_Gothenburg--Gelred.pdf">GelRed Nucleic acid stain</a></li> | <li class="list"><a href="https://static.igem.org/mediawiki/2016/1/1e/T--Chalmers_Gothenburg--Gelred.pdf">GelRed Nucleic acid stain</a></li> | ||
− | <li class="list">Kanamycin sulfate</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/5/5c/T--Chalmers_Gothenburg--Kanamycin.pdf">Kanamycin sulfate</a></li> |
− | <li class="list">Spectinomycin hydrochloride</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/f/f8/T--Chalmers_Gothenburg--Spectinomycin.pdf">Spectinomycin hydrochloride</a></li> |
</ul> | </ul> | ||
<h3 class="sidetitle" id="Risk_assessments">KLARA Risk assessments</h3> | <h3 class="sidetitle" id="Risk_assessments">KLARA Risk assessments</h3> | ||
<ul> | <ul> | ||
− | <li class="list">Dishwasher</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/f/f9/T--Chalmers_Gothenburg--Dishwasher.pdf">Dishwasher</a></li> |
− | <li class="list">-80°C freezer</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/c/c8/T--Chalmers_Gothenburg--Freezer.pdf">-80°C freezer</a></li> |
− | <li class="list">Bench-top autoclave</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/a/ae/T--Chalmers_Gothenburg--Benchtopautoclave.pdf">Bench-top autoclave</a></li> |
− | <li class="list">Centrifugal vacuum vaporator</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/5/57/T--Chalmers_Gothenburg--Centrifugalvacuumvaporator.pdf">Centrifugal vacuum vaporator</a></li> |
− | <li class="list">Centrifuge (Avanti J-20 XP)</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/4/4d/T--Chalmers_Gothenburg--Centrifugeavanti.pdf">Centrifuge (Avanti J-20 XP)</a></li> |
− | <li class="list">Centrifuge (Sigma 4K15)</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/d/d8/T--Chalmers_Gothenburg--Centrifugesigma.pdf">Centrifuge (Sigma 4K15)</a></li> |
− | <li class="list">Gel electrophoresis with GelRed</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/f/fd/T--Chalmers_Gothenburg--Gelfored.pdf">Gel electrophoresis with GelRed</a></li> |
− | <li class="list">Gel electrophoresis</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/1/11/T--Chalmers_Gothenburg--Gelfo.pdf">Gel electrophoresis</a></li> |
− | <li class="list">Heat block</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/7/72/T--Chalmers_Gothenburg--Heatblock.pdf">Heat block</a></li> |
− | <li class="list">Rotary shakers, Incubators</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/7/77/T--Chalmers_Gothenburg--Incubators.pdf">Rotary shakers, Incubators</a></li> |
− | <li class="list">Small centrifuges</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/1/10/T--Chalmers_Gothenburg--Smallcentrifuges.pdf">Small centrifuges</a></li> |
− | <li class="list">LAF bench</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/1/14/T--Chalmers_Gothenburg--Lafbench.pdf">LAF bench</a></li> |
− | <li class="list">Thermal cycler (PCR)</li> | + | <li class="list"><a href="https://static.igem.org/mediawiki/2016/f/f0/T--Chalmers_Gothenburg--PCR.pdf">Thermal cycler (PCR)</a></li> |
</ul> | </ul> | ||
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Latest revision as of 13:45, 19 October 2016
Before starting the wet lab part of the project all team members had to take part in a safety lecture held by the lab manager at the Systems and synthetic biology department. During this lecture the team learned how to handle chemicals and waste in the lab, we also had a lab tour where the safety equipment were introduced. During the lab tour all the responsible research engineers were present and gave us introductions to all of the different lab equipments they are responsible for.
As a safety measurement, each team member had to read the materials safety data sheet for the materials used and the KLARA risk assessments of the machines handled during the summer. Each member then had to sign the risk declaration to testify that one had taken part of this information and gain access to the laboratory.
Risk declaration
MSDS - Material Safety Data Sheet
- Chloramphenicol
- Ampicillin
- G418 disulfate salt colution
- GelRed Nucleic acid stain
- Kanamycin sulfate
- Spectinomycin hydrochloride