Difference between revisions of "Team:Dalhousie Halifax NS/Safety"

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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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  <h1 id="title" style="text-align:center; font-family:lato-black;">BIOSAFETY</h1>
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  <p id="navigators" style="text-align: center; font-size: 35pt; font-family:lato-black;"><a id="navigators" href="#labsafety">Lab Safety</a>  |  <a id="navigators" href="#EnvironmentSamples">Environmental Samples</a></p>
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    <h2 style="font-family:lato-black; color:#9D3412;">Lab Safety</h2>
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  <h4>The lab we used for our project this year is an Level Two Biosafety lab associated with Dalhousie University and with the Nova Scotia Health Authority (NSHA). Because of this, we had many resources for maintaining our health and safety such as Dalhousie’s Biosafety Office, our supervisors Dr. Lois Murray, Dr. John Rohde, and Dr. Zhenyu Cheng, and the Biosafety Guidelines that pertain to NSHA labs.</h4>
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    <h4>In order to maintain our safety, we were consistently wearing personal protective equipment that includes lab coats, gloves closed-toed shoes and, when necessary, safety glasses. </h4>
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  <h4>Where it was applicable to do so, we used biological safety cabinets (BSC) with HEPA filters. The BSC - and the laminar flow hoods – were used to protect us and samples from contamination. All biohazardous consumable materials, such as pipette tips, serological pipette’s, and used agar plates, were place in biohazardous waste bags and disposed of according to NSHA policy. All contaminated liquids and reusable materials were autoclaved when appropriate and decontaminated with 70% ethanol or 10% bleach when autoclaving was not an option. Situations where autoclaving was not available includes decontaminating plastics and glass beads for examples. Lab surfaces were always decontaminated with 70% ethanol before and after use.</h4>
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<h5>Safe Project Design</h5>
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  <h2 style="text-align: center;">Environmental Samples</h2>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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  <h4>Our project provides unique biosafety challenges because we are working with fecal samples containing many unknown bacteria. Being unaware of what a sample contains makes things complicated when attempting to be bio-safe. To ensure our safety, we were vigilant with our decontamination procedures and use of personal protective equipment. Contact with the fecal samples was limited to use for DNA extraction and serial dilutions for plating.
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h4>All bacteria cultured from the fecal samples were unknown, so care was taken with these samples to ensure our safety. These samples were worked with inside a BSC and were not left to overgrow. They were disposed of through biohazardous waste bags according to NSHA policy.</h4>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 15:42, 19 October 2016

Dalhousie iGEM 2016

BIOSAFETY

Lab Safety

The lab we used for our project this year is an Level Two Biosafety lab associated with Dalhousie University and with the Nova Scotia Health Authority (NSHA). Because of this, we had many resources for maintaining our health and safety such as Dalhousie’s Biosafety Office, our supervisors Dr. Lois Murray, Dr. John Rohde, and Dr. Zhenyu Cheng, and the Biosafety Guidelines that pertain to NSHA labs.

In order to maintain our safety, we were consistently wearing personal protective equipment that includes lab coats, gloves closed-toed shoes and, when necessary, safety glasses.

Where it was applicable to do so, we used biological safety cabinets (BSC) with HEPA filters. The BSC - and the laminar flow hoods – were used to protect us and samples from contamination. All biohazardous consumable materials, such as pipette tips, serological pipette’s, and used agar plates, were place in biohazardous waste bags and disposed of according to NSHA policy. All contaminated liquids and reusable materials were autoclaved when appropriate and decontaminated with 70% ethanol or 10% bleach when autoclaving was not an option. Situations where autoclaving was not available includes decontaminating plastics and glass beads for examples. Lab surfaces were always decontaminated with 70% ethanol before and after use.

Environmental Samples

Our project provides unique biosafety challenges because we are working with fecal samples containing many unknown bacteria. Being unaware of what a sample contains makes things complicated when attempting to be bio-safe. To ensure our safety, we were vigilant with our decontamination procedures and use of personal protective equipment. Contact with the fecal samples was limited to use for DNA extraction and serial dilutions for plating.

All bacteria cultured from the fecal samples were unknown, so care was taken with these samples to ensure our safety. These samples were worked with inside a BSC and were not left to overgrow. They were disposed of through biohazardous waste bags according to NSHA policy.