4.1 clone 6 extracted DNA (which presented a good size strip on electrophoresis)
4.1 clone 6 extracted DNA (which presented a good size strip on electrophoresis)
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6 clones of each of the other transformations [[Team:Paris_Saclay/Notebook/June/28#pPS16_001|pPS16_001, pPS16_002, pPS16_003, pPS16_005 and pPS16_006]] were placed in 4mL of LB added to Ampicillin (50µg/mL) at 37°C, 180RPM. For 2.2 just 2 clones were cultivated because there was not more colony. To obtain more colonies, another transformation (Heat shock competent cells, section protocols) was made with 50µL of cells, 5µL of plasmid. 50µL of transformed bacteria were displayed on LB + Ampicillin (50µg/mL)dishes.
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6 clones of each of the other transformations [[Team:Paris_Saclay/Notebook/June/28#pPS16_001|pPS16_001, pPS16_002, pPS16_003, pPS16_005 and pPS16_006]] were grown in 4mL of LB with Ampicillin (50µg/mL) at 37°C, 180rpm. For pPS16_002 only 2 clones were cultivated because there was not more colony. To obtain more colonies, another [[Team:Paris_Saclay/Experiments#HeatShockCompetent|transformation]] was made with 50µL of cells and 5µL of plasmid. 50µL of transformed bacteria were plated on LB + Ampicillin (50µg/mL).
