Difference between revisions of "Team:Paris Saclay/Notebook/July/13"

(Biobrick characterization)
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Glycerol stocks for DH5α tra,sformed with DS-NMcas, DS-SPcasN-, DS-ST1casN- and DS-TDcasN-
  
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By Laetitia
  
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8 stocks were made:
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2 clones (Cl 1 and Cl 2) by cas:
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* Cl1 and Cl2 of  DS-NMcas
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* Cl1 and Cl2 of  DS-SPcasN-
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* Cl1 and Cl2 of  DS-ST1casN-
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* Cl1 and Cl2 of  DS-TDcasN-
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For 1 glycerol stock:
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1mL of liquid culture
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500 μL of glycerol 60%
  
 
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Revision as of 14:48, 13 July 2016

Wednesday 13th July

Lab work

Getting DNA closer

Glycerol stocks for DH5α tra,sformed with DS-NMcas, DS-SPcasN-, DS-ST1casN- and DS-TDcasN-

By Laetitia

8 stocks were made: 2 clones (Cl 1 and Cl 2) by cas:

  • Cl1 and Cl2 of DS-NMcas
  • Cl1 and Cl2 of DS-SPcasN-
  • Cl1 and Cl2 of DS-ST1casN-
  • Cl1 and Cl2 of DS-TDcasN-


For 1 glycerol stock: 1mL of liquid culture 500 μL of glycerol 60%



Biobrick characterization

Visualization