Difference between revisions of "Team:NAWI-Graz/Proof"

 
(7 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{NAWI-Graz}}
 
−
 
 
<html>
 
<html>
 +
<head>
 +
 +
<!-- Basic -->
 +
<meta charset="utf-8">
 +
<meta http-equiv="X-UA-Compatible" content="IE=edge">
 +
 +
<title>NAWI-Graz @ iGem - Description</title>
 +
 +
<meta name="keywords" content="NAWI-Graz, Description" />
 +
<meta name="description" content="Project-Description">
 +
 +
<!-- Favicon -->
 +
<link rel="shortcut icon" href="img/favicon.ico" type="image/x-icon" />
 +
<link rel="apple-touch-icon" href="img/apple-touch-icon.png">
 +
 +
<!-- Mobile Metas -->
 +
<meta name="viewport" content="width=device-width, minimum-scale=1.0, maximum-scale=1.0, user-scalable=no">
 +
 +
<!-- Web Fonts  -->
 +
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400,600,700,800%7CShadows+Into+Light" rel="stylesheet" type="text/css">
 +
 +
<!-- Vendor CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/bootstrap/css/bootstrap.min.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/font-awesome/css/font-awesome.min.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/simple-line-icons/css/simple-line-icons.min.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/owl.carousel/assets/owlcarouselmin.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/owl.carousel/assets/owlthemedefaultmin.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/magnific-popup/magnific-popup.min.css&action=raw&ctype=text/css">
 +
 +
<!-- Theme CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-elements.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-blog.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-shop.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-animate.css&action=raw&ctype=text/css">
 +
 +
<!-- Current Page CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/rs-plugin/css/settings.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/rs-plugin/css/layers.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/rs-plugin/css/navigation.css&action=raw&ctype=text/css">
 +
 +
<!-- Skin CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/skins/skin-medical.css&action=raw&ctype=text/css">
 +
 +
<!-- Demo CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/demos/demo-medical.css&action=raw&ctype=text/css">
 +
 +
<!-- Theme Custom CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/custom.css&action=raw&ctype=text/css">
 +
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/simple-line-icons/css/simple-line-icons.css&action=raw&ctype=text/css">
 +
 +
 +
<!-- Head Libs -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/modernizr/modernizrmin?action=raw&ctype=text/javascript"></script>
 +
 +
 +
 +
</head>
 +
<body data-spy="scroll" data-target="#navSecondary" data-offset="170">
 +
 +
<div class="body">
 +
<header id="header" class="header-narrow" data-plugin-options='{"stickyEnabled": true, "stickyEnableOnBoxed": true, "stickyEnableOnMobile": true, "stickyStartAt": 35, "stickySetTop": "-35px", "stickyChangeLogo": false}'>
 +
<div class="header-body">
 +
<div class="header-top header-top header-top-style-3 header-top-custom">
 +
<div class="container">
 +
<nav class="header-nav-top pull-right">
 +
<ul class="nav nav-pills">
 +
<li class="hidden-xs">
 +
<span class="ws-nowrap">Petersgasse 12, Graz</span>
 +
</li>
 +
<li class="hidden-xs">
 +
<span class="ws-nowrap"> <a class="text-decoration-none" href="#">NAWI Graz</a></span>
 +
</li>
 +
</ul>
 +
</nav>
 +
</div>
 +
</div>
 +
<div class="header-container container">
 +
<div class="header-row">
 +
<div class="header-column">
 +
<div class="header-logo">
 +
<a href="#">
 +
<img alt="iGem" width="90" height="70" src="/wiki/images/a/a4/T--NAWI-Graz--igem.png">
 +
</a>
 +
</div>
 +
</div>
 +
<div class="header-column">
 +
<div class="header-row">
 +
<div class="header-nav pt-xs">
 +
<button class="btn header-btn-collapse-nav" data-toggle="collapse" data-target=".header-nav-main">
 +
<i class="fa fa-bars"></i>
 +
</button>
 +
<div class="header-nav-main header-nav-main-effect-1 header-nav-main-sub-effect-1 collapse">
 +
<nav>
 +
<ul class="nav nav-pills" id="mainNav">
 +
<li class="activedropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz">
 +
Home
 +
</a>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="#">
 +
Team
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Team">Team</a></li>
 +
<li><a href="/Team:NAWI-Graz/Collaborations">Collaborations</a></li>
 +
</ul>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="#">
 +
Project
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Description">Description</a></li>
 +
<li><a href="/Team:NAWI-Graz/Design">Design</a></li>
 +
<li><a href="/Team:NAWI-Graz/Experiments">Experiments</a></li>
 +
<li><a href="/Team:NAWI-Graz/Proof">Proof of concept</a></li>
 +
<li><a href="/Team:NAWI-Graz/Demonstration">Demonstration</a></li>
 +
<li><a href="/Team:NAWI-Graz/Results">Results</a></li>
 +
<li><a href="/Team:NAWI-Graz/Notebook">Notebook</a></li>
 +
</ul>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="#">
 +
Parts
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Basic_Part">Basic_Parts</a></li>
 +
<li><a href="/Team:NAWI-Graz/Composite_Part">Composite Part</a></li>
 +
<li><a href="/Team:NAWI-Graz/Part_Collection">Part Collection</a></li>
 +
</ul>
 +
</li>
 +
<li class="dropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz/Safety">
 +
Safety
 +
</a>
 +
</li>
 +
<li class="dropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz/Attributions">
 +
Attributions
 +
</a>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="/Team:NAWI-Graz/Human_Practices">
 +
Human Practices
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Human_Practices">Human Practices</a></li>
 +
<li><a href="/Team:NAWI-Graz/Silver">Silver</a></li>
 +
 +
</ul>
 +
</li>
 +
<li class="dropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz/Sponsor"
 +
Sponsoring
 +
</a>
 +
</li>
 +
</ul>
 +
</nav>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</header>
 +
 +
<div role="main" class="main">
 +
 +
 +
<section class="section-custom-medical">
 +
<div class="container">
 +
<div class="row mt-xlg pt-xlg mb-xlg pb-xs">
 +
<div class="col-sm-8 col-md-8">
 +
<!--
 +
<h2 class="font-weight-semibold mb-xs">Our project</h2>
 +
<p class="lead font-weight-normal">The Last Colinator</p>-->
 +
 +
 +
 +
<h2 class="mt-xl mb-none"><span class="alternative-font font-size-md">Proteomics</span></h2>
 +
<p>
 +
The check if the seven proteins, chromoprotein red/blue, ccdB, mazF, ccdA, mazE and dam methylase were expressed we used SDS-PAGE. It was also important to see, if the proteins especially the toxins were secreted to the media. The chromoproteins can be found at about 25kda, at the size of 32kDa we can find the dam methylase. The ccdB has a weight of about 11kDa.
 +
</p>
 +
 +
<p>The two antitoxins and mazE are smaller than 10kDA, when seeing the picture in real we could find small bands lower than 10kDA. Unfortinately no time was left for puring a higher concentrated SDS gel.</p>
 +
 +
<p>Although we cannot be 100% sure that all proteins we needed were expressed by <i>E.coli</i> we can be really sure that our insilico worked. For all composite parts one protein can be found on the gel. In case of the toxin part we found the dam methylase wich was located downstream the toxin. In case of the antitoxin composite part we saw the red chromoprotein on the SDS and also with the coloured colonies. </p><br>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/c/c4/T--NAWI-Graz--F10.png" alt="proof10" class="img-responsive">
 +
<p>Figure 10 SDS-PAGE useing the growthmedia from our strains. We used the pure solution and the sperarated media in lower 10kDA and bigger than 10kDA for analysis. We can a little lower than the 35kDA standard the dam methylase. At the size of 25kDA we find the chromoproteins from the differnen strains. At 11kDA the ccdB toxin of the blue strain can be detected. </p>
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
<html>
 +
<head>
 +
 +
<!-- Basic -->
 +
<meta charset="utf-8">
 +
<meta http-equiv="X-UA-Compatible" content="IE=edge">
 +
 +
<title>NAWI-Graz @ iGem - Description</title>
 +
 +
<meta name="keywords" content="NAWI-Graz, Description" />
 +
<meta name="description" content="Project-Description">
 +
 +
<!-- Favicon -->
 +
<link rel="shortcut icon" href="img/favicon.ico" type="image/x-icon" />
 +
<link rel="apple-touch-icon" href="img/apple-touch-icon.png">
 +
 +
<!-- Mobile Metas -->
 +
<meta name="viewport" content="width=device-width, minimum-scale=1.0, maximum-scale=1.0, user-scalable=no">
 +
 +
<!-- Web Fonts  -->
 +
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400,600,700,800%7CShadows+Into+Light" rel="stylesheet" type="text/css">
 +
 +
<!-- Vendor CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/bootstrap/css/bootstrap.min.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/font-awesome/css/font-awesome.min.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/simple-line-icons/css/simple-line-icons.min.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/owl.carousel/assets/owlcarouselmin.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/owl.carousel/assets/owlthemedefaultmin.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/magnific-popup/magnific-popup.min.css&action=raw&ctype=text/css">
  
 +
<!-- Theme CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-elements.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-blog.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-shop.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-animate.css&action=raw&ctype=text/css">
  
 +
<!-- Current Page CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/rs-plugin/css/settings.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/rs-plugin/css/layers.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/rs-plugin/css/navigation.css&action=raw&ctype=text/css">
  
 +
<!-- Skin CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/skins/skin-medical.css&action=raw&ctype=text/css">
  
 +
<!-- Demo CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/demos/demo-medical.css&action=raw&ctype=text/css">
  
 +
<!-- Theme Custom CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/custom.css&action=raw&ctype=text/css">
  
−
<div class="column full_size">
+
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/simple-line-icons/css/simple-line-icons.css&action=raw&ctype=text/css">
 +
 +
 +
<!-- Head Libs -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/modernizr/modernizrmin?action=raw&ctype=text/javascript"></script>
 +
 +
  
 +
</head>
 +
<body data-spy="scroll" data-target="#navSecondary" data-offset="170">
  
−
<p>
+
<div class="body">
−
iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.  
+
<header id="header" class="header-narrow" data-plugin-options='{"stickyEnabled": true, "stickyEnableOnBoxed": true, "stickyEnableOnMobile": true, "stickyStartAt": 35, "stickySetTop": "-35px", "stickyChangeLogo": false}'>
−
</p>
+
<div class="header-body">
 +
<div class="header-top header-top header-top-style-3 header-top-custom">
 +
<div class="container">
 +
<nav class="header-nav-top pull-right">
 +
<ul class="nav nav-pills">
 +
<li class="hidden-xs">
 +
<span class="ws-nowrap">Petersgasse 12, Graz</span>
 +
</li>
 +
<li class="hidden-xs">
 +
<span class="ws-nowrap"> <a class="text-decoration-none" href="#">NAWI Graz</a></span>
 +
</li>
 +
</ul>
 +
</nav>
 +
</div>
 +
</div>
 +
<div class="header-container container">
 +
<div class="header-row">
 +
<div class="header-column">
 +
<div class="header-logo">
 +
<a href="#">
 +
<img alt="iGem" width="90" height="70" src="/wiki/images/a/a4/T--NAWI-Graz--igem.png">
 +
</a>
 +
</div>
 +
</div>
 +
<div class="header-column">
 +
<div class="header-row">
 +
<div class="header-nav pt-xs">
 +
<button class="btn header-btn-collapse-nav" data-toggle="collapse" data-target=".header-nav-main">
 +
<i class="fa fa-bars"></i>
 +
</button>
 +
<div class="header-nav-main header-nav-main-effect-1 header-nav-main-sub-effect-1 collapse">
 +
<nav>
 +
<ul class="nav nav-pills" id="mainNav">
 +
<li class="activedropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz">
 +
Home
 +
</a>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="#">
 +
Team
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Team">Team</a></li>
 +
<li><a href="/Team:NAWI-Graz/Collaborations">Collaborations</a></li>
 +
</ul>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="#">
 +
Project
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Description">Description</a></li>
 +
<li><a href="/Team:NAWI-Graz/Design">Design</a></li>
 +
<li><a href="/Team:NAWI-Graz/Experiments">Experiments</a></li>
 +
<li><a href="/Team:NAWI-Graz/Proof">Proof of concept</a></li>
 +
<li><a href="/Team:NAWI-Graz/Demonstration">Demonstration</a></li>
 +
<li><a href="/Team:NAWI-Graz/Results">Results</a></li>
 +
<li><a href="/Team:NAWI-Graz/Notebook">Notebook</a></li>
 +
</ul>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="#">
 +
Parts
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Basic_Part">Basic_Parts</a></li>
 +
<li><a href="/Team:NAWI-Graz/Composite_Part">Composite Part</a></li>
 +
<li><a href="/Team:NAWI-Graz/Part_Collection">Part Collection</a></li>
 +
</ul>
 +
</li>
 +
<li class="dropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz/Safety">
 +
Safety
 +
</a>
 +
</li>
 +
<li class="dropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz/Attributions">
 +
Attributions
 +
</a>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="/Team:NAWI-Graz/Human_Practices">
 +
Human Practices
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Human_Practices">Human Practices</a></li>
 +
<li><a href="/Team:NAWI-Graz/Silver">Silver</a></li>
 +
 +
</ul>
 +
</li>
 +
<li class="dropdown-full-color dropdown-secondary">
 +
<a href="#Team:NAWI-Graz/Sponsor">
 +
Sponsoring
 +
</a>
 +
</li>
 +
</ul>
 +
</nav>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</header>
  
 +
<div role="main" class="main">
 +
 +
 +
 +
 +
 +
<footer id="footer" class="m-none">
 +
<div class="container">
 +
 +
 +
<div class="row mb-xlg">
 +
<div class="col-md-12">
  
−
<h4> What should we do for our proof of concept? </h4>
+
<div class="row show-grid">
−
<p>  
+
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/b/b2/T--NAWI-Graz--StefitzLogo.png" alt class="img-responsive" /></span></div>
−
You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
+
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/9/9f/Thermo-Fisher.jpeg" alt class="img-responsive" /></span></div>
−
</p>
+
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/1/1a/Zeta.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/0/00/Microsynth.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/3/3b/T--NAWI-Graz--Schursches-Insel-Cafe-Logo.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/3/39/T--NAWI-Graz--snapgene.png" alt class="img-responsive" /> </span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/e/e5/KFU.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/5/5f/TU_Graz.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/7/76/T--NAWI-Graz--servolab.png" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/1/1e/T--NAWI-Graz--bartelt.png" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/4/44/T--NAWI-Graz--greiner-logo.png" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/1/11/T--NAWI-Graz--IDT-Logo.png" alt class="img-responsive" /></span></div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="row">
 +
<div class="col-md-3">
 +
<h4 class="mb-xlg">Location</h4>
 +
<p>
 +
NAWI Graz<br>
 +
Petersgasse 12<br>
 +
8010 Graz, AUT<br>
 +
</p>
 +
</div>
 +
<div class="col-md-3 col-md-offset-1">
 +
<div class="contact-details">
 +
<h4 class="mb-xlg">Mail address</h4>
 +
<a class="text-decoration-none" href="mailto:igem.nawigraz@gmail.com">
 +
igem.nawigraz@gmail.com
 +
</a>
 +
</div>
 +
</div>
 +
<div class="col-md-2">
 +
<h4 class="mb-xlg">Social Media</h4>
 +
<ul class="social-icons">
 +
<li class="social-icons-facebook">
 +
<a href="https://www.facebook.com/igemteamgraz2016/?fref=ts" target="_blank" title="Facebook">
 +
<i class="fa fa-facebook"></i>
 +
</a>
 +
</li>
 +
</ul>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="footer-copyright pt-md pb-md">
 +
<div class="container">
 +
<div class="row">
 +
<div class="col-md-12 center m-none">
 +
<p>© Copyright 2016. All Rights Reserved.</p>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</footer>
 +
</div>
 +
<!-- Vendor -->
 +
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery/jquerymin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery.appear/jqueryappearmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery.easing/jqueryeasingmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery-cookie/jquerycookiemin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/bootstrap/js/bootstrapmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/common/commonmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery.validation/jqueryvalidationmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery.lazyload/jquerylazyloadmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/isotope/jqueryisotopemin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/owl.carousel/owlcarouselmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/magnific-popup/jquerymagnificpopupmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/vide/videmin?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Theme Base, Components and Settings -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/theme?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Current Page Vendor and Views -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/rs-plugin/js/jquerythemepunchtoolsmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/rs-plugin/js/jquerythemepunchrevolutionmin?action=raw&ctype=text/javascript"></script>
  
−
</div>
+
<!-- Current Page Vendor and Views -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/views/viewcontact?action=raw&ctype=text/javascript"></script>
  
 +
<!-- Demo -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/demos/demomedical?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Theme Custom -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/custom?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Theme Initialization Files -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/themeinit?action=raw&ctype=text/javascript"></script>
  
  
−
</div>
 
  
 +
</body>
 
</html>
 
</html>

Latest revision as of 17:32, 19 October 2016

NAWI-Graz @ iGem - Description

Proteomics

The check if the seven proteins, chromoprotein red/blue, ccdB, mazF, ccdA, mazE and dam methylase were expressed we used SDS-PAGE. It was also important to see, if the proteins especially the toxins were secreted to the media. The chromoproteins can be found at about 25kda, at the size of 32kDa we can find the dam methylase. The ccdB has a weight of about 11kDa.

The two antitoxins and mazE are smaller than 10kDA, when seeing the picture in real we could find small bands lower than 10kDA. Unfortinately no time was left for puring a higher concentrated SDS gel.

Although we cannot be 100% sure that all proteins we needed were expressed by E.coli we can be really sure that our insilico worked. For all composite parts one protein can be found on the gel. In case of the toxin part we found the dam methylase wich was located downstream the toxin. In case of the antitoxin composite part we saw the red chromoprotein on the SDS and also with the coloured colonies.


proof10

Figure 10 SDS-PAGE useing the growthmedia from our strains. We used the pure solution and the sperarated media in lower 10kDA and bigger than 10kDA for analysis. We can a little lower than the 35kDA standard the dam methylase. At the size of 25kDA we find the chromoproteins from the differnen strains. At 11kDA the ccdB toxin of the blue strain can be detected.

NAWI-Graz @ iGem - Description

Location

NAWI Graz
Petersgasse 12
8010 Graz, AUT

Mail address

igem.nawigraz@gmail.com

Social Media

© Copyright 2016. All Rights Reserved.