Difference between revisions of "Team:NAWI-Graz/Composite Part"

(Prototype team page)
 
 
(9 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{NAWI-Graz}}
 
 
<html>
 
<html>
 +
<head>
  
 +
<!-- Basic -->
 +
<meta charset="utf-8">
 +
<meta http-equiv="X-UA-Compatible" content="IE=edge">
  
−
<div class="column full_size judges-will-not-evaluate">
+
<title>NAWI-Graz @ iGem - Description</title>
−
<h3>★  ALERT! </h3>
+
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Composite Part special prize</a>. </p>
+
  
 +
<meta name="keywords" content="NAWI-Graz, Description" />
 +
<meta name="description" content="Project-Description">
  
 +
<!-- Favicon -->
 +
<link rel="shortcut icon" href="img/favicon.ico" type="image/x-icon" />
 +
<link rel="apple-touch-icon" href="img/apple-touch-icon.png">
  
−
<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
+
<!-- Mobile Metas -->
−
</div>
+
<meta name="viewport" content="width=device-width, minimum-scale=1.0, maximum-scale=1.0, user-scalable=no">
  
 +
<!-- Web Fonts  -->
 +
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400,600,700,800%7CShadows+Into+Light" rel="stylesheet" type="text/css">
  
 +
<!-- Vendor CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/bootstrap/css/bootstrap.min.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/font-awesome/css/font-awesome.min.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/simple-line-icons/css/simple-line-icons.min.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/owl.carousel/assets/owlcarouselmin.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/owl.carousel/assets/owlthemedefaultmin.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/magnific-popup/magnific-popup.min.css&action=raw&ctype=text/css">
  
 +
<!-- Theme CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-elements.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-blog.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-shop.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/theme-animate.css&action=raw&ctype=text/css">
  
 +
<!-- Current Page CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/rs-plugin/css/settings.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/rs-plugin/css/layers.css&action=raw&ctype=text/css">
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/rs-plugin/css/navigation.css&action=raw&ctype=text/css">
  
−
<div class="column full_size">
+
<!-- Skin CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/skins/skin-medical.css&action=raw&ctype=text/css">  
  
 +
<!-- Demo CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/demos/demo-medical.css&action=raw&ctype=text/css">
  
 +
<!-- Theme Custom CSS -->
 +
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/css/custom.css&action=raw&ctype=text/css">
  
−
<p>
+
<link rel="stylesheet" type="text/css" href="https://2016.igem.org/wiki/index.php?title=Team:NAWI-Graz/vendor/simple-line-icons/css/simple-line-icons.css&action=raw&ctype=text/css">
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
−
</p>
+
 +
<!-- Head Libs -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/modernizr/modernizrmin?action=raw&ctype=text/javascript"></script>
 +
 +
  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
+
</head>
 +
<body data-spy="scroll" data-target="#navSecondary" data-offset="170">
  
 +
<div class="body">
 +
<header id="header" class="header-narrow" data-plugin-options='{"stickyEnabled": true, "stickyEnableOnBoxed": true, "stickyEnableOnMobile": true, "stickyStartAt": 35, "stickySetTop": "-35px", "stickyChangeLogo": false}'>
 +
<div class="header-body">
 +
<div class="header-top header-top header-top-style-3 header-top-custom">
 +
<div class="container">
 +
<nav class="header-nav-top pull-right">
 +
<ul class="nav nav-pills">
 +
<li class="hidden-xs">
 +
<span class="ws-nowrap">Petersgasse 12, Graz</span>
 +
</li>
 +
<li class="hidden-xs">
 +
<span class="ws-nowrap"> <a class="text-decoration-none" href="#">NAWI Graz</a></span>
 +
</li>
 +
</ul>
 +
</nav>
 +
</div>
 +
</div>
 +
<div class="header-container container">
 +
<div class="header-row">
 +
<div class="header-column">
 +
<div class="header-logo">
 +
<a href="#">
 +
<img alt="iGem" width="90" height="70" src="/wiki/images/a/a4/T--NAWI-Graz--igem.png">
 +
</a>
 +
</div>
 +
</div>
 +
<div class="header-column">
 +
<div class="header-row">
 +
<div class="header-nav pt-xs">
 +
<button class="btn header-btn-collapse-nav" data-toggle="collapse" data-target=".header-nav-main">
 +
<i class="fa fa-bars"></i>
 +
</button>
 +
<div class="header-nav-main header-nav-main-effect-1 header-nav-main-sub-effect-1 collapse">
 +
<nav>
 +
<ul class="nav nav-pills" id="mainNav">
 +
<li class="activedropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz">
 +
Home
 +
</a>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="#">
 +
Team
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Team">Team</a></li>
 +
<li><a href="/Team:NAWI-Graz/Collaborations">Collaborations</a></li>
 +
</ul>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="#">
 +
Project
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Description">Description</a></li>
 +
<li><a href="/Team:NAWI-Graz/Silver">Design</a></li>
 +
<li><a href="/Team:NAWI-Graz/Gold">Experiments</a></li>
 +
<li><a href="/Team:NAWI-Graz/Proof">Proof of concept</a></li>
 +
<li><a href="/Team:NAWI-Graz/Demonstration">Demonstration</a></li>
 +
<li><a href="/Team:NAWI-Graz/Results">Results</a></li>
 +
<li><a href="/Team:NAWI-Graz/Notebook">Notebook</a></li>
 +
</ul>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="#">
 +
Parts
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Basic_Part">Basic_Parts</a></li>
 +
<li><a href="/Team:NAWI-Graz/Composite_Part">Composite Part</a></li>
 +
<li><a href="/Team:NAWI-Graz/Part_Collection">Part Collection</a></li>
 +
</ul>
 +
</li>
 +
<li class="dropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz/Safety">
 +
Safety
 +
</a>
 +
</li>
 +
<li class="dropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz/Attributions">
 +
Attributions
 +
</a>
 +
</li>
 +
<li class="dropdown dropdown-full-color dropdown-secondary">
 +
<a class="dropdown-toggle" href="/Team:NAWI-Graz/Human_Practices">
 +
Human Practices
 +
</a>
 +
<ul class="dropdown-menu">
 +
<li><a href="/Team:NAWI-Graz/Human_Practices">Human Practices</a></li>
 +
<li><a href="/Team:NAWI-Graz/Silver">Silver</a></li>
 +
 +
</ul>
 +
</li>
 +
<li class="dropdown-full-color dropdown-secondary">
 +
<a href="/Team:NAWI-Graz/Sponsor">
 +
Sponsoring
 +
</a>
 +
</li>
 +
</ul>
 +
</nav>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</header>
  
 +
<div role="main" class="main">
 +
 +
 +
<section class="section-custom-medical">
 +
<div class="container">
 +
<div class="row mt-xlg pt-xlg mb-xlg pb-xs">
 +
<div class="col-sm-8 col-md-8">
 +
<!--
 +
<h2 class="font-weight-semibold mb-xs">Our project</h2>
 +
<p class="lead font-weight-normal">The Last Colinator</p>-->
  
−
<div class="highlight">
+
<h2 class="mt-xl mb-none"><span class="alternative-font font-size-md">Composite parts sent to the registry</span></h2>
−
<h4>Note</h4>
+
<p>
−
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
+
<b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2142000" target="_blank">BBa_K2142000</a> </b><br>
 +
This part was used to make our <i>E.coli</i> resistant against the mazF toxin they should produce. For this reason the expressed the mazE antitoxin (BBa_K2142001). Further we used Anderson promoters and RBS to be sure our proteins are expressed constitutive. This was very important for the reason the Antitoxin has a really short half-life and the chromoprotein was used to detect the winner. At the end of the part we used a bidirectional double terminator, which also terminated the transcription of BBa_K2142005.</p>
 +
<p><b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2142005" target="_blank">BBa_K2142005</a></b><br>
 +
In our project we used this composite part to let our <i>E.coli</i> attack with the mazF toxin (BBa_K2142003). Further this part contains a Dam methylase (BBa_K2142004) for the reason this protein leads due to over expression to an increased mutation rate. For the reason toxin production and mutation can cause problems during assembling and testing the expression of the proteins can be induced with IPTG. This part belongs to BBa_K2142000.</p>
 +
<p><b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2142007" target="_blank">BBa_K2142007</a></b><br>
 +
This part was used to make our <i>E.colis</i> resistant against the ccdB toxin they should produce. For this reason they expressed the (BBa_K2142006). Further we used Anderson promoters and RBS to be sure our proteins are expressed constitutive. This was very important for the reason the Antitoxin has a real short half-life and the chromoprotein was used to detect the winner. At the end of the part we used a bidirectional double terminator, which also terminated the transcription of BBa_K2142005.
  
−
</div>
+
</p>
  
−
</div>
+
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
 +
 +
<footer id="footer" class="m-none">
 +
<div class="container">
 +
 +
 +
<div class="row mb-xlg">
 +
<div class="col-md-12">
  
 +
<div class="row show-grid">
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/b/b2/T--NAWI-Graz--StefitzLogo.png" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/9/9f/Thermo-Fisher.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/1/1a/Zeta.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/0/00/Microsynth.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/3/3b/T--NAWI-Graz--Schursches-Insel-Cafe-Logo.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/3/39/T--NAWI-Graz--snapgene.png" alt class="img-responsive" /> </span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/e/e5/KFU.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/5/5f/TU_Graz.jpeg" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/7/76/T--NAWI-Graz--servolab.png" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/1/1e/T--NAWI-Graz--bartelt.png" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/4/44/T--NAWI-Graz--greiner-logo.png" alt class="img-responsive" /></span></div>
 +
<div class="col-md-1"><span class="show-grid-block"><img src="/wiki/images/1/11/T--NAWI-Graz--IDT-Logo.png" alt class="img-responsive" /></span></div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div class="row">
 +
<div class="col-md-3">
 +
<h4 class="mb-xlg">Location</h4>
 +
<p>
 +
NAWI Graz<br>
 +
Petersgasse 12<br>
 +
8010 Graz, AUT<br>
 +
</p>
 +
</div>
 +
<div class="col-md-3 col-md-offset-1">
 +
<div class="contact-details">
 +
<h4 class="mb-xlg">Mail address</h4>
 +
<a class="text-decoration-none" href="mailto:igem.nawigraz@gmail.com">
 +
igem.nawigraz@gmail.com
 +
</a>
 +
</div>
 +
</div>
 +
<div class="col-md-2">
 +
<h4 class="mb-xlg">Social Media</h4>
 +
<ul class="social-icons">
 +
<li class="social-icons-facebook">
 +
<a href="https://www.facebook.com/igemteamgraz2016/?fref=ts" target="_blank" title="Facebook">
 +
<i class="fa fa-facebook"></i>
 +
</a>
 +
</li>
 +
</ul>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="footer-copyright pt-md pb-md">
 +
<div class="container">
 +
<div class="row">
 +
<div class="col-md-12 center m-none">
 +
<p>© Copyright 2016. All Rights Reserved.</p>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</footer>
 +
</div>
 +
<!-- Vendor -->
 +
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery/jquerymin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery.appear/jqueryappearmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery.easing/jqueryeasingmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery-cookie/jquerycookiemin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/bootstrap/js/bootstrapmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/common/commonmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery.validation/jqueryvalidationmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/jquery.lazyload/jquerylazyloadmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/isotope/jqueryisotopemin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/owl.carousel/owlcarouselmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/magnific-popup/jquerymagnificpopupmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/vide/videmin?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Theme Base, Components and Settings -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/theme?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Current Page Vendor and Views -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/rs-plugin/js/jquerythemepunchtoolsmin?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/vendor/rs-plugin/js/jquerythemepunchrevolutionmin?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Current Page Vendor and Views -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/views/viewcontact?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Demo -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/demos/demomedical?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Theme Custom -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/custom?action=raw&ctype=text/javascript"></script>
 +
 +
<!-- Theme Initialization Files -->
 +
<script type="text/javascript" src="https://2016.igem.org/Template:NAWI-Graz/js/themeinit?action=raw&ctype=text/javascript"></script>
 +
 +
 +
 +
 +
<!-- Google Analytics: Change UA-XXXXX-X to be your site's ID. Go to http://www.google.com/analytics/ for more information.
 +
<script>
 +
(function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){
 +
(i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o),
 +
m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m)
 +
})(window,document,'script','//www.google-analytics.com/analytics.js','ga');
 +
 +
ga('create', 'UA-12345678-1', 'auto');
 +
ga('send', 'pageview');
 +
</script>
 +
-->
 +
 +
 +
</body>
 
</html>
 
</html>

Latest revision as of 17:46, 19 October 2016

NAWI-Graz @ iGem - Description

Composite parts sent to the registry

BBa_K2142000
This part was used to make our E.coli resistant against the mazF toxin they should produce. For this reason the expressed the mazE antitoxin (BBa_K2142001). Further we used Anderson promoters and RBS to be sure our proteins are expressed constitutive. This was very important for the reason the Antitoxin has a really short half-life and the chromoprotein was used to detect the winner. At the end of the part we used a bidirectional double terminator, which also terminated the transcription of BBa_K2142005.

BBa_K2142005
In our project we used this composite part to let our E.coli attack with the mazF toxin (BBa_K2142003). Further this part contains a Dam methylase (BBa_K2142004) for the reason this protein leads due to over expression to an increased mutation rate. For the reason toxin production and mutation can cause problems during assembling and testing the expression of the proteins can be induced with IPTG. This part belongs to BBa_K2142000.

BBa_K2142007
This part was used to make our E.colis resistant against the ccdB toxin they should produce. For this reason they expressed the (BBa_K2142006). Further we used Anderson promoters and RBS to be sure our proteins are expressed constitutive. This was very important for the reason the Antitoxin has a real short half-life and the chromoprotein was used to detect the winner. At the end of the part we used a bidirectional double terminator, which also terminated the transcription of BBa_K2142005.

Location

NAWI Graz
Petersgasse 12
8010 Graz, AUT

Mail address

igem.nawigraz@gmail.com

Social Media

© Copyright 2016. All Rights Reserved.