Difference between revisions of "Team:WPI Worcester"

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<h3><b>R.I.C.E CRISPRs</b></h3>
 
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<p>Nonsense and missense mutations cause numerous incurable diseases, including Cystic Fibrosis and Duchenne Muscular Dystrophy, that affect millions of people worldwide. Gene therapy is the most promising treatment for these genetics diseases. The majority of current gene therapy techniques focus on correction of DNA sequences; our project focuses on direct editing of RNA sequences. Targeting RNA is advantageous because it permits a tunable and reversible editing system that can repair multiple mutations while circumventing the process of homology-directed repair. We have constructed dCas9 fusions to the editing enzymes APOBEC1 and ADAR1/2 to enable CRISPR-targeted C-to-U and A-to-I edits to RNA, respectively. We have designed and thoroughly characterized a series of GFP-based reporters that enable the detection and quantification of RNA editing by these dCas9 fusions. Our project outlines the theoretical framework for a novel, tunable, and reversible gene editing strategy with myriad applications.</p>
 
  
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
 
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
 
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
 
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<h5> Styling your wiki </h5>
 
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<td align="center"> <a href="https://2016.igem.org/Team:WPI_Worcester/Description">
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<img src="https://static.igem.org/mediawiki/2016/9/9a/Project_description.jpg" style="width:200px;height:150px;"></a> </td>
  
  
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<td align="center"> <a href  ="https://2016.igem.org/Team:WPI_Worcester/Design">
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<img src="https://static.igem.org/mediawiki/2016/7/78/Pencil-typography-black-design.jpg" style="width:200px;height:150px;"></a> </td>
  
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<h5> Editing your wiki </h5>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> To edit this page, go to wiki tools and click edit. To edit other pages, go to that page, and go to the wiki tools like before and press edit again</p>
 
  
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<td align="center"> <a href="https://2016.igem.org/Team:WPI_Worcester/Results">
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<img src="https://static.igem.org/mediawiki/2016/d/d1/T--WPI_Worcester--Results.jpeg" style="width:200px;height:150px;"></a> </td>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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UPLOAD FILES
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<td align="center"> <a href  ="https://2016.igem.org/Team:WPI_Worcester/Human_Practices">
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<h2  style="text-align:center;"><b>Abstract</b></h2>
  
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<h4 align="center">Nonsense and missense mutations cause numerous incurable diseases, including Cystic Fibrosis and Duchenne Muscular Dystrophy, that affect millions of people worldwide. Gene therapy is the most promising treatment for these genetic diseases. The majority of current gene therapy techniques focus on correction of DNA sequences; our project focuses on direct editing of RNA sequences. Targeting RNA is advantageous because it permits a tunable and reversible editing system that can repair multiple mutations while circumventing the process of homology-directed repair. We have constructed dCas9 fusions to the editing enzymes APOBEC1 and ADAR1/2 to enable CRISPR-targeted C-to-U and A-to-I edits to RNA, respectively. We have designed and thoroughly characterized a series of GFP-based reporters that enable the detection and quantification of RNA editing by these dCas9 fusions. Our project outlines the theoretical framework for a novel, tunable, and reversible gene editing strategy with myriad applications.</h4>
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Latest revision as of 19:54, 19 October 2016



Abstract

Nonsense and missense mutations cause numerous incurable diseases, including Cystic Fibrosis and Duchenne Muscular Dystrophy, that affect millions of people worldwide. Gene therapy is the most promising treatment for these genetic diseases. The majority of current gene therapy techniques focus on correction of DNA sequences; our project focuses on direct editing of RNA sequences. Targeting RNA is advantageous because it permits a tunable and reversible editing system that can repair multiple mutations while circumventing the process of homology-directed repair. We have constructed dCas9 fusions to the editing enzymes APOBEC1 and ADAR1/2 to enable CRISPR-targeted C-to-U and A-to-I edits to RNA, respectively. We have designed and thoroughly characterized a series of GFP-based reporters that enable the detection and quantification of RNA editing by these dCas9 fusions. Our project outlines the theoretical framework for a novel, tunable, and reversible gene editing strategy with myriad applications.