Difference between revisions of "Team:Peking/Composite Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Composite Part special prize</a>. </p>
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        <title>Parts</title>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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        <meta name="description" content="Wiki of Peking iGEM 2016" />
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</div>
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        <meta name="author" content="Li Jiamian & Wang Yuqing"/>
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<p>
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        <link rel="stylesheet" href="https://2016.igem.org/Template:Peking/css/default?action=raw&ctype=text/css"/>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Crosslinking" >Crosslinking</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption" >Uranyl adsorption</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Model/GelPoint" > Model of Gel Point </a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Model/MassDistribution" > Model of Mass Distribution</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Software" >Software</a></li>
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                <div class="twelve columns centered text-center">
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                    <h1>Parts</h1>
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                    <p class="title1" style="text-align:center"></p>
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                </div>
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                                    <h4><a href="javascript:void(0);" onclick="naver('favor')">Favorite&nbsp;Parts</a></h4>
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                                    <h4><a href="javascript:void(0);" onclick="naver('table')">Part&nbsp;Table</a></h4>
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                                    <h4><a href="javascript:void(0);" onclick="naver('collection')">Part&nbsp;Collection</a></h4>
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                            <a id="favor"></a>
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                            <div class="texttitle">Favorite Parts</div>
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                            <br/>
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                            <figure>
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                                <p style="text-align:center;"><img style="width: 80% ;" src="https://static.igem.org/mediawiki/2016/6/63/T--Peking--image_partfig1c.png" alt=""/></p>
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                                <figcaption style="text-align:center;">
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                                    Fig. 1. Basic structure of the following parts
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                                </figcaption>
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                            </figure>
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                            <br/>
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                            <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a></h3>
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                            <hr style="border:2px dashed; height:2px" color="#666666">
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                            <figure>
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                                <p style="text-align:center;"><img style="width: 60% ;" src=" https://static.igem.org/mediawiki/2016/9/94/T--Peking--image_partfig2c.png" alt=""/></p>
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                                <figcaption style="text-align:center;">
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                                    Fig. 2. 3A: Triple spytag SUP: Super uranyl-binding protein
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                                </figcaption>
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                            </figure>
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                            <p class="lead add-bottom" style="color:#5E5656">Super uranyl-binding protein (SUP) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the uranyl-binding capacity in our polymer network system. Paired with triple spycatcher (<a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a>) and tripled spytag with mSA (<a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a>) our polymer network system is constructed. </p>
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                            <br/>
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                            <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a></h3>
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                            <hr style="border:2px dashed; height:2px" color="#666666">
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                            <figure>
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                                <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/4/4d/T--Peking--image_partfig3c.png" alt=""/></p>
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                                <figcaption style="text-align:center;">
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                                    Fig. 3. 3B Triple spycatcher
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                                </figcaption>
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                            </figure>
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                            <p class="lead add-bottom" style="color:#5E5656">Triple spycatcher was spaced by 15*(VPGVG). Paired with triple spytag (<a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a>) and tripled spytag with mSA (<a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a>) our polymer network system is constructed.</p>
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                            <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K1989002">BBa_K1989002</a></h3>
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                            <hr style="border:2px dashed; height:2px" color="#666666">
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                            <figure>
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                                <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/4/43/T--Peking--image_partfig4c.png" alt=""/></p>
 +
                                <figcaption style="text-align:center;">
 +
                                    Fig. 4. 3A: Triple spytag mSA: Monomeric streptavidin
 +
                                </figcaption>
 +
                            </figure>
 +
                            <p class="lead add-bottom" style="color:#5E5656">Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the recyclable capacity in our polymer network system. Paired with triple spycatcher (<a href="http://parts.igem.org/Part:BBa_K1989001">BBa_K1989001</a>) and tripled spytag with SUP (<a href="http://parts.igem.org/Part:BBa_K1989000">BBa_K1989000</a>) our polymer network system is constructed.</p>
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                            <p class="lead add-bottom" style="color:#5E5656">2016 Peking iGEM team members devoted themselves to constructing a part library of the multifunctional material. We have not only submitted all of new basic Spy network parts, but also provided multiple composite parts combining Spy network with other metal binding proteins and fluorescent proteins. We also constructed a library of signal peptides and validated the secretion capacity of our material.</p>
 +
                            <p class="lead add-bottom" style="color:#5E5656">Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM team.</p>
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Latest revision as of 20:07, 19 October 2016

Parts

Parts

Favorite Parts

Fig. 1. Basic structure of the following parts

BBa_K1989000

Fig. 2. 3A: Triple spytag SUP: Super uranyl-binding protein

Super uranyl-binding protein (SUP) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the uranyl-binding capacity in our polymer network system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with mSA (BBa_K1989002) our polymer network system is constructed.


BBa_K1989001

Fig. 3. 3B Triple spycatcher

Triple spycatcher was spaced by 15*(VPGVG). Paired with triple spytag (BBa_K1989000) and tripled spytag with mSA (BBa_K1989002) our polymer network system is constructed.

BBa_K1989002

Fig. 4. 3A: Triple spytag mSA: Monomeric streptavidin

Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the recyclable capacity in our polymer network system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with SUP (BBa_K1989000) our polymer network system is constructed.


Part Table

Loading…..

Part Collection

2016 Peking iGEM team members devoted themselves to constructing a part library of the multifunctional material. We have not only submitted all of new basic Spy network parts, but also provided multiple composite parts combining Spy network with other metal binding proteins and fluorescent proteins. We also constructed a library of signal peptides and validated the secretion capacity of our material.

Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM team.