Difference between revisions of "Team:Toulouse France"

 
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<font color="white"><p style="text-align:center; font-size:18px; vertical-align:middle;">iGEM Toulouse Team presents<br><i>Paleotilis, a shield for the Lascaux cave</i>
 
<font color="white"><p style="text-align:center; font-size:18px; vertical-align:middle;">iGEM Toulouse Team presents<br><i>Paleotilis, a shield for the Lascaux cave</i>
 
<br><br> Back to the origins<br><br><p></font>
 
<br><br> Back to the origins<br><br><p></font>
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<br>What if iGEM stepped 18,000 years back?  
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<br><b style="font-size:18px;">What if iGEM stepped 18,000 years back? </b>
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<br><br>At that time, cavemen painted extraordinary frescoes on the Lascaux cave (France). The cave remained stable until its discovery in 1940, which deeply disturbed its ecosystem. It was closed shortly after mainly because of contamination by fungi that cause colored spots on the frescoes. Nowadays, mechanical and chemical solutions are daily used and the cave is still in danger due to their limited efficiency. <br>
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<br><br>At that time, cavemen painted extraordinary frescoes on the Lascaux cave (France). The cave remained stable until its discovery in 1940, which deeply disturbed its ecosystem. It was closed shortly after mainly because of contamination by fungi that causes colored spots on the frescoes. Nowadays, mechanical and chemical solutions are monthly used and the cave is still in danger due to their limited efficiency. <br>
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<br><br>At iGEM Toulouse, we thought about an innovative biological solution based on the last advances in synthetic biology. It consists in an engineered <i>Bacillus subtilis</i> bacterium that develops from bacterial organisms present in the cave. It releases antifungals when in close viscinity of fungi. Since we care about our environment, an inducible riboswitch-based double toxin/antitoxin system was created to prevent DNA transmission and a physical device was designed to confine our strain.
+
<br><br>At iGEM Toulouse, we thought about an innovative biological solution based on the last advances in synthetic biology. It consists in an engineered <i>Bacillus subtilis</i> strain which grow on bacterial organisms present in the cave. It releases antifungals when in close viscinity of fungi. Since we care about environment, an inducible riboswitch-based double toxin/antitoxin system was created to prevent DNA transmission and a physical device was designed to confine our strain.
 
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<div class="line-gallerie-project">
 
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<a href="https://2016.igem.org/Team:Toulouse_France/Context" class="part-little-projet">
 
<a href="https://2016.igem.org/Team:Toulouse_France/Context" class="part-little-projet">
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<div class="title-part-projet">Project</div>
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<div class="title-part-projet" style="text-align:center;">Project</div>
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<div style="position:absolute; top:90px; left:34px; color:white; font-family:'Open Sans'; font-size:16px; width:200px; line-height:24px;">The project <b>Paleotilis</b> is composed of three modules: <b>Predation</b>, <b>Antifungals</b>, and <b>Confinement</b>. By following this link you will learn more about these constructions.</div>
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<div style="text-align:justify; word-wrap: break-word;position:absolute; top:90px; left:34px; color:white; font-family:'Open Sans'; font-size:16px; width:80%; line-height:24px;">Discover the context of the <b>Paleotilis</b> project, and the three modules of its design: <b>Predation</b>, <b>Antifungals</b>, and <b>Confinement</b>. Click on the link to learn more about these constructions.<br><br></div>
 
<div style="border-bottom: 1px solid #fff; color:white; font-family:'Open Sans'; position:absolute; bottom:25px; right:22px; font-size:16px;">Read more</div>
 
<div style="border-bottom: 1px solid #fff; color:white; font-family:'Open Sans'; position:absolute; bottom:25px; right:22px; font-size:16px;">Read more</div>
 
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<a href="https://2016.igem.org/Team:Toulouse_France/Experiments" class="part-little-projet">
 
<a href="https://2016.igem.org/Team:Toulouse_France/Experiments" class="part-little-projet">
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<div class="title-part-projet">Results</div>
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<div class="title-part-projet" style="text-align:center;">Results</div>
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<div  style="position:absolute; top:90px; left:34px; color:white; font-family:'Open Sans'; font-size:16px; width:200px; line-height:24px">The different biobricks we have constructed have been tested to measure their efficiency. There you can have an overview of the results.</div>
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<div  style="text-align:justify;  word-wrap: break-word; position:absolute; top:90px; left:34px; color:white; font-family:'Open Sans'; font-size:16px; width:80%; line-height:24px">The different biobricks we constructed have been tested to measure their efficiency. There you will have an overview of the results.<br></div>
 
<div style="border-bottom: 1px solid #fff; color:white; font-family:'Open Sans'; position:absolute; bottom:28px; left:34px; font-size:16px;">Read more</div>
 
<div style="border-bottom: 1px solid #fff; color:white; font-family:'Open Sans'; position:absolute; bottom:28px; left:34px; font-size:16px;">Read more</div>
 
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<a href="https://2016.igem.org/Team:Toulouse_France/Human_Practices" class="part-little-projet">
 
<a href="https://2016.igem.org/Team:Toulouse_France/Human_Practices" class="part-little-projet">
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<div class="title-part-projet">Human Practices</div>
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<div class="title-part-projet" style="text-align:center;">Human Practices</div>
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<div  style="position:absolute; top:90px; left:34px; color:white; font-family:'Open Sans'; font-size:16px; width:200px; line-height:24px">Lot of projects and events have been done by the iGEM Toulouse team. We aimed <b>to explain synthetic biology, to debate about the use of GMO and to transmit what we have learnt</b>.</div>
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<div  style="text-align:justify;  word-wrap: break-word; position:absolute; top:90px; left:34px; color:white; font-family:'Open Sans'; font-size:16px; width:80%; line-height:24px">Lot of projects and events have been done by the iGEM Toulouse team. We aimed <b>to explain synthetic biology, to debate about the use of GMOs and to transmit what we have learnt</b>.<br><br></div>
 
<div style="border-bottom: 1px solid #fff; color:white; font-family:'Open Sans'; position:absolute; bottom:25px; right:34px; font-size:16px;">Read more </div>
 
<div style="border-bottom: 1px solid #fff; color:white; font-family:'Open Sans'; position:absolute; bottom:25px; right:34px; font-size:16px;">Read more </div>
 
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<h3><strong>Achievements</strong></h3>
 
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<ul><h3><strong>Bronze medal</strong></h3></ul>
 
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<li>Register the team to iGEM, have fun during the summer and attend the Giant Jamboree with smile</li>
 
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<li>Submit successfully <a target="blank" href="https://igem.org/2016_Judging_Form?id=1937"> the Judging form</a></li>
 
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<li>Create a page in the wiki with attributions to every people that helped us in different ways</li>
 
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<li>Document a new BioBrick and add it to the registry parts: <a href="http://parts.igem.org/Part:BBa_K1937002">pSB1C3-OriKan (BBa_K1937002)</a> corresponds to a shuttle vector between <i>E.coli</i> and <i>B.subtilis</i>
 
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<br>Registry (from <a target="blank" href="https://2016.igem.org/Team:Toulouse_France/Composite_parts"> See Parts</a>)</li>
 
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<li>Create and share a complete description of the team’s project in the Wiki</li>
 
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<li><a target="blank" href="https://2016.igem.org/Team:Toulouse_France/Attributions"> Attributions:</a> our project could be
 
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possible thanks to several persons who helped us in different ways and we are very grateful</li>
 
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<li>Plan to present a beautiful poster and a pleasant Prezi presentation at the Giant Jamboree</li>
 
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<ul><h3><strong>Silver medal</strong></h3></ul>
 
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<li>Present strong commitment to Human Practices for <b>Science Popularization</b> by
 
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participating to different events in France, for <b>Education</b> by developing an education
 
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kit, for <b>Safety</b> by minimizing the risk of incidents in the lab and for <b>Ethics</b> by creating
 
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a theatre play</li>
 
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<li>Experimentally validate three new BioBricks: <a href="http://parts.igem.org/Part:BBa_K1937002">pSB1C3-OriKan (BBa_ K1937002)</a>, <a href="http://parts.igem.org/Part:BBa_K1937001">pSB(BS)0K-mini (BBa_ K1937001)</a> and <a href="http://parts.igem.org/Part:BBa_K1937003">PnagA (BBa_K1937003)</a> worked successfully as expected</li>
 
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<li>Take part to active collaborations for the scientific community: creation of <b>Multilanguage protocol videos</b> thanks to 7 others teams</li>
 
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<center><a class="button-home" href="https://2016.igem.org/Team:Toulouse_France/HP/Silver" style="border: 1px solid #282828;-webkit-border-radius: 5px;-moz-border-radius: 5px;border-radius: 5px; padding: 5px 15px; color: #282828; text-decoration: none; font-size: 17px; background: none; display: block; width: 200px;">HP/Silver</a></center>
 
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<ul><h3><strong>Gold medal</strong></h3></ul>
 
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<li>Innovative human practices: </li>
 
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<strong>-</strong> Active participating in <a target="blank" href="https://2015.igem.org/Team:Toulouse_France/Engagement">ExpoScience international</a>??? <a target="blank" href="">exciting perspectives</a><br>
 
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<strong>-</strong> Our project is based on a <b>deep</b><a target="blank" href="https://2015.igem.org/Team:Toulouse_France/Human_Practices#Ethics"> Ethical reflection</a><br>
 
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<strong>-</strong> <a target="blank" href="https://2015.igem.org/Team:Toulouse_France/Attributions#Media">Discussions with people</a> to fit the best with real issue<br>
 
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<strong>-</strong> <a target="blank" href="https://2015.igem.org/Team:Toulouse_France/Engagement">Communication with kids</a><br>
 
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</ul>
 
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<li>Integrated innovative human practices: integration of discussions with Lascaux Cave experts and deep ethical reflection helped by an ethicist into our project</li>
 
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<li>Improve the function of the existing pSB0K-mini plasmid <a href="http://parts.igem.org/Part:BBa_K823026">(Biobrick BBa_K823026)</a>, from the 2012 Munich team, in a smaller plasmid pSB0K-mini <a href="http://parts.igem.org/Part:BBa_K1937001">(Biobrick BBa_ K1937001)</a> and enter it in the Registry</li>
 
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<li><a target="blank" href="https://2016.igem.org/Team:Toulouse_France/Collaborations">Collaborations:</a> </li>
 
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<ul>
 
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<strong>-</strong> Myhuyhuiujio <a target="blank" href=""> ednfhjzel <a target="blank" href=""> <br>
 
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<strong>-</strong> <a target="blank" href="https://2016.igem.org/Team:Imperial_College/Software#mainContent">Imperial College team:</a> we studied the optimal growth conditions of <i>B. subtilis</i> and <i>E. coli</i> strains to document their software <b>A.L.I.C.E.</b><br>
 
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<center><a class="button-home" href="https://2016.igem.org/Team:Toulouse_France/HP/Gold" style="border: 1px solid #282828;-webkit-border-radius: 5px;-moz-border-radius: 5px;border-radius: 5px; padding: 5px 15px; color: #282828; text-decoration: none; font-size: 17px; background: none; display: block; width: 200px;">HP/Gold</a></center>
 
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<center><a class="button-home" href="https://2016.igem.org/Team:Toulouse_France/Integrated_Practices" style="border: 1px solid #282828;-webkit-border-radius: 5px;-moz-border-radius: 5px;border-radius: 5px; padding: 5px 15px; color: #282828; text-decoration: none; font-size: 17px; background: none; display: block; width: 200px;">Integrated Pracices</a></center>
 
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<center><a class="button-home" href="https://2016.igem.org/Team:Toulouse_France/Engagement" style="border: 1px solid #282828;-webkit-border-radius: 5px;-moz-border-radius: 5px;border-radius: 5px; padding: 5px 15px; color: #282828; text-decoration: none; font-size: 17px; background: none; display: block; width: 200px;">Public Engagement</a></center>
 
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</ul></ul></ul>
 
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Latest revision as of 20:11, 19 October 2016

iGEM Toulouse 2016


iGEM Toulouse Team presents
Paleotilis, a shield for the Lascaux cave

Back to the origins


What if iGEM stepped 18,000 years back?

At that time, cavemen painted extraordinary frescoes on the Lascaux cave (France). The cave remained stable until its discovery in 1940, which deeply disturbed its ecosystem. It was closed shortly after mainly because of contamination by fungi that causes colored spots on the frescoes. Nowadays, mechanical and chemical solutions are monthly used and the cave is still in danger due to their limited efficiency.


At iGEM Toulouse, we thought about an innovative biological solution based on the last advances in synthetic biology. It consists in an engineered Bacillus subtilis strain which grow on bacterial organisms present in the cave. It releases antifungals when in close viscinity of fungi. Since we care about environment, an inducible riboswitch-based double toxin/antitoxin system was created to prevent DNA transmission and a physical device was designed to confine our strain.
Project
Discover the context of the Paleotilis project, and the three modules of its design: Predation, Antifungals, and Confinement. Click on the link to learn more about these constructions.

Read more
Results
The different biobricks we constructed have been tested to measure their efficiency. There you will have an overview of the results.
Read more
Human Practices
Lot of projects and events have been done by the iGEM Toulouse team. We aimed to explain synthetic biology, to debate about the use of GMOs and to transmit what we have learnt.

Read more



Contacts
    🏠 INSA Toulouse
    135, Avenue de Rangueil
    31077 TOULOUSE Cedex 4
    France
  • ☎ +00 (33) 6 13 64 89 40
  • ✉ igemtoulouse2016@gmail.com