Difference between revisions of "Team:Ionis Paris/Projectsafety"

 
(8 intermediate revisions by 4 users not shown)
Line 7: Line 7:
 
         <link href='https://fonts.googleapis.com/css?family=Open+Sans:400,600,700,600italic,400italic,700italic,300,300italic' rel='stylesheet' type='text/css'>
 
         <link href='https://fonts.googleapis.com/css?family=Open+Sans:400,600,700,600italic,400italic,700italic,300,300italic' rel='stylesheet' type='text/css'>
  
        <!-- Nos feuilles de style -->
+
          <!-- Nos feuilles de style -->
        <link rel="stylesheet" type="text/css" href="https://2016.igem.org/Template:IONIS_Paris-test?action=raw&ctype=text/css" />
+
    <link rel="stylesheet" type="text/css" href="https://2016.igem.org/Template:IONIS_Paris-style-css-template?action=raw&ctype=text/css" />
        <link rel="stylesheet" type="text/css" href="https://2016.igem.org/Template:IONIS_Paris-style-css?action=raw&ctype=text/css" />
+
    <!-- Version minifiée dans le bon ordre de toutes les feuilles de style du template -->
 +
    <link rel="stylesheet" type="text/css" href="https://2016.igem.org/Template:IONIS_Paris-style-css?action=raw&ctype=text/css" />
 
    
 
    
 
         <!-- ====BREADCUM START==== -->  
 
         <!-- ====BREADCUM START==== -->  
Line 17: Line 18:
 
                     <div class="col-sm-12">
 
                     <div class="col-sm-12">
 
                         <div class="banner_title">
 
                         <div class="banner_title">
                             <h1>Project Safety</h1>
+
                             <h1 style="color :#960c38;" id="back_to_the_top">Project Safety</h1>
 
                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
Line 26: Line 27:
 
          
 
          
 
         <!-- ====START BLOG TABLE==== -->
 
         <!-- ====START BLOG TABLE==== -->
         <section class="blog_area section-padding">
+
         <section id="about" class="about_area section-padding">
 
             <div class="container">
 
             <div class="container">
 
                 <div class="row">
 
                 <div class="row">
                    <div class="blog_grid_area">
+
     
                        <div class="col-xs-12 col-sm-9">
+
                    <div class="col-xs-12">
                            <div class="bloggrid_right">
+
       
                                <div class="blog_top">
+
 
                                    <h4 class="blog_topHd" style="margin-top:20px">
+
                                    <h2 class="sec">Safe Project Design</h2>
                                      Safe Project Design
+
                          </div>
                                    </h4>
+
 
 +
<div class="col-sm-9">
 
                                  
 
                                  
  
<h3>Choosing a non-pathogenic chassis</h3>
+
<h2 class="secHd">Choosing a non-pathogenic chassis</h2>
  
<p>For our project, we chose to use chassis lab strains of  E. coli bacteria (DH5a and BL21 strains). As non-pathogenic to humans, these E. coli strains belong to Risk Group 1 and thus require only basic precautions. We chose these organisms for convenience reasons: their genomes are entirely sequenced (we knew that the sigma factor required for our transcription factor biosensor was in the bacteria), they are easy to grow and safe to handle.</p>
+
<p>For our project, we chose to use chassis lab strains of  <i>E. coli</i> bacteria (DH5a and BL21 strains). As non-pathogenic to humans, these E. coli strains belong to Risk Group 1 and thus require only basic precautions. We chose these organisms for convenience reasons: their genomes are entirely sequenced (we knew that the sigma factor required for our transcription factor biosensor was in the bacteria), they are easy to grow and safe to handle.</p>
  
 
                              
 
                              
<h3>Choosing parts that will not harm humans/animals/plants</h3>
+
<h2 class="secHd">Choosing parts that will not harm humans/animals/plants</h2>
  
  
 
<p>We designed our project in a way that it should not include parts that could be harmful to humans, animals or plants. Our project involves a XylR transcription factor and a luciferase reporter and neither of these parts are known to have safety issues.</p>
 
<p>We designed our project in a way that it should not include parts that could be harmful to humans, animals or plants. Our project involves a XylR transcription factor and a luciferase reporter and neither of these parts are known to have safety issues.</p>
  
<h3>Biological Containment</h3>
+
<h2 class="secHd">Biological Containment</h2>
  
 
<p>Our project is designed for a use in everyday life which is why we were particularly aware of safety issues.
 
<p>Our project is designed for a use in everyday life which is why we were particularly aware of safety issues.
The main risks we have identified can be linked to the use of biological elements. We are indeed planning to use genetically engineered bacteria, which implies an environmental dimension, given that those bacteria would be used in the field. This was our biggest challenge when we started the competition and in order to solve it we thought of a system to address this. We developed a containment method that will allow us to separate the bacteria from the environment. The container will be an enclosed device that allows air to be sampled and presented to the bacteria while preventing their accidental release (see <a href="2016.Team:Ionis_Paris/Hardware">Hardware</a>).
+
The main risks we have identified can be linked to the use of biological elements. We planned to use genetically engineered bacteria and therefore an ethical dimension had to be taken in account. <br/>
The first BioBrick tests were done in the laboratory under simulated environmental conditions. In the meantime, our Engineering team is developing a containment method similar to an airlock that would allow an efficient sampling of the air with no bacteria escaping the collection tube. However no field tests were made during the iGEM competition.
+
As those modified bacteria will be used in the field, our biggest challenge was to safely contain them. To adress this problem, we developed a containment method that will allow us to separate the bacteria from the environment. The container will be an enclosed device that allows air to be sampled and presented to the bacteria while preventing their accidental release (see <a href="2016.Team:Ionis_Paris/Hardware">Hardware</a>).
 +
The first BioBrick tests were done in the laboratory under simulated environmental conditions. In the meantime, our Engineering team was developing a containment method similar to an airlock that would allow an efficient sampling of the air with no bacteria escaping the collection tube. However no field tests were made during the iGEM competition. <br/>
 
Although our biggest challenge comes from biology, we also identified a mechanical risk in the drone. There is the possibility that the drone will have an accident. We thought of a security system for this event that will allow us to handle such accident without harm and pollution (see <a href="2016.Team:Ionis_Paris/Hardware">Hardware)</a>.</p>
 
Although our biggest challenge comes from biology, we also identified a mechanical risk in the drone. There is the possibility that the drone will have an accident. We thought of a security system for this event that will allow us to handle such accident without harm and pollution (see <a href="2016.Team:Ionis_Paris/Hardware">Hardware)</a>.</p>
  
<h3>Thinking about the future</h3>
+
<h2 class="secHd">Thinking about the future</h2>
 +
 
 
<p>We are also aware of the issues caused by the use of a drone in urban areas and the problems of selling our system to the general public. These issues motivated us to think of an economic model based on the sale of a service, not a product. In this way, our system will only be use by trained person belonging to our company and we will sell only data.</p>
 
<p>We are also aware of the issues caused by the use of a drone in urban areas and the problems of selling our system to the general public. These issues motivated us to think of an economic model based on the sale of a service, not a product. In this way, our system will only be use by trained person belonging to our company and we will sell only data.</p>
  
 +
</div>
 +
</div>
 +
</div>
 +
</div>
  
  
  
  
 
 
 
                                </div>
 
                               
 
                                </div>
 
                           
 
                             
 
                        </div>
 
                      </div>
 
                    </div>
 
                </div>
 
            </div>
 
 
         </section>
 
         </section>
 
         <!-- ====END BLOG TABLE==== -->
 
         <!-- ====END BLOG TABLE==== -->
 
          
 
          
 
   
 
   
        <!-- ====START SOCIAL Link==== -->
+
  <!-- ====START SOCIAL Link==== -->
        <div class="footer_social">
+
    <div class="footer_social">
            <div class="container-fluid">
+
        <div class="container-fluid">
                <div class="row">
+
            <div class="row">
                    <ul class="ft_top clearfix">
+
                <ul class="ft_top clearfix">
                        <li class="waves-effect waves-light">
+
                    <li class="waves-effect waves-light">
                            <a href="https://www.facebook.com/ionisigem/?fref=ts" target="_blank">
+
                        <a href="https://www.facebook.com/ionisigem/?fref=ts" target="_blank">
                                <i class="zmdi zmdi-facebook "></i>Facebook
+
                            <i class="zmdi zmdi-facebook "></i>Facebook
                            </a>
+
                        </a>
                        </li>  
+
                    </li>
                        <li class="waves-effect waves-light">
+
                    <li class="waves-effect waves-light">
                            <a href="https://twitter.com/igem_ionis" target="_blank">
+
                        <a href="https://twitter.com/igem_ionis" target="_blank">
                                <i class="zmdi zmdi-twitter"></i>Twitter
+
                            <i class="zmdi zmdi-twitter"></i>Twitter
                            </a>
+
                        </a>
                        </li>  
+
                    </li>
                        <li class="waves-effect waves-light">
+
                    <li class="waves-effect waves-light">
                            <a href="https://www.youtube.com/channel/UC0RyQsB5YpweSRBYQlAdZDA" target="_blank">
+
                        <a href="https://www.youtube.com/channel/UC0RyQsB5YpweSRBYQlAdZDA" target="_blank">
                                <i class="zmdi zmdi-youtube"></i>youtube
+
                            <i class="zmdi zmdi-youtube"></i>YouTube
                            </a>
+
                        </a>
                        </li>  
+
                    </li>
                        <li class="waves-effect waves-light">
+
                    <li class="waves-effect waves-light">
                            <a href="https://www.linkedin.com/company/igem-ionis" target="_blank">
+
                        <a href="https://www.linkedin.com/company/igem-ionis" target="_blank">
                                <i class="zmdi zmdi-linkedin"></i>LinkedIn
+
                            <i class="zmdi zmdi-linkedin"></i>LinkedIn
                            </a>
+
                        </a>
                        </li>  
+
                    </li>
+
 
                    </ul>
+
                </ul>
                </div>
+
 
             </div>
 
             </div>
 
         </div>
 
         </div>
        <!-- ====END FOOTER TOP==== -->
+
    </div>
       
+
    <!-- ====END FOOTER TOP==== -->
        <!-- ====START FOOTER AREA==== -->
+
 
        <footer class="footer_area">
+
    <!-- ====START FOOTER AREA==== -->
            <div class="footer_middle">
+
    <footer class="footer_area">
                <div class="container">
+
        <div class="footer_middle">
                    <div class="row">
+
            <div class="container">
                        <div class="col-xs-12">
+
                <div class="row">
                            <div class="scroll_area">
+
                    <div class="col-xs-12">
                                <div class="sroll_top">
+
                        <div class="scroll_area">
                                  <a href="#ancre"> <i class="zmdi zmdi-chevron-up btn waves-effect"> </i> </a
+
                            <div class="sroll_top">
                                </div>
+
                                <a href="#ancre"> <i class="zmdi zmdi-chevron-up btn waves-effect"> </i> </a>
 
                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
 +
                </div>
 +
                <div class="row">
 
                     <div class="row">
 
                     <div class="row">
                      <div class="row">
 
 
                         <div class="col-lg-5 col-md-5 col-sm-12">
 
                         <div class="col-lg-5 col-md-5 col-sm-12">
 
                             <div class="middle_content">
 
                             <div class="middle_content">
                                 <h4>IONIS IGEM</h4>
+
                                 <h4>iGEM IONIS</h4>
                                 <p> We're a group of six different schools from the IONIS Education Group. For this competition we wanted to take advantages of the multiple schools and activity field given by the IONIS education group to create a solid project.</p>
+
                                 <p> We're a group of six different schools from the IONIS Education Group. For this
                              <a href="https://2016.igem.org/Team:Ionis_Paris/Team">Read More</a>
+
                                    competition we wanted to take advantage of the multiple schools and fields of activity
 +
                                    given by the IONIS education group to create a solid project.</p>
 +
                                <a href="https://2016.igem.org/Team:Ionis_Paris/Team">Read More</a>
 
                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
             
+
 
 
                         <div class="col-lg-3 col-lg-offset-1 col-md-4 col-sm-7">
 
                         <div class="col-lg-3 col-lg-offset-1 col-md-4 col-sm-7">
 
                             <div class="footer_Widgets">
 
                             <div class="footer_Widgets">
Line 146: Line 143:
 
                                             <a href="mailto:igem.ionis@gmail.com">email: igem.ionis@gmail.com</a>
 
                                             <a href="mailto:igem.ionis@gmail.com">email: igem.ionis@gmail.com</a>
 
                                         </li>
 
                                         </li>
                                     
+
 
 
                                     </ul>
 
                                     </ul>
 
                                 </div>
 
                                 </div>
 
                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
 +
                    <div class="col-lg-3 col-md-3 col-sm-5">
 +
                            <div class="footer_Widgets">
 +
                                <h4>Download the app</h4>
 +
                                <a href="https://itunes.apple.com/us/app/quantifly/id1166875690?ls=1&mt=8"
 +
                                  target="_blank" style="target-new: tab;">
 +
                                    <figure class="widget_gallery">
 +
                                        <div class="RXcircleEffect"></div>
 +
                                        <img src="https://static.igem.org/mediawiki/2016/8/8d/IONIS_IGEM_paris_logo_apple.png"
 +
                                            alt="" />
 +
                                        <figcaption>
 +
                                            <i class="zmdi zmdi-link"></i>
 +
                                        </figcaption>
 +
                                    </figure>
 +
                                </a>
 +
                                <a href="https://play.google.com/store/apps/details?id=fr.plnech.quantifly&hl=en"
 +
                                  target="_blank" style="target-new: tab;">
 +
                                    <figure class="widget_gallery">
 +
                                        <div class="RXcircleEffect"></div>
 +
                                        <img src="https://static.igem.org/mediawiki/2016/6/65/IONIS_IGEM_paris_google_play.png"
 +
                                            alt="" />
 +
                                        <figcaption>
 +
                                            <i class="zmdi zmdi-link"></i>
 +
                                        </figcaption>
 +
                                    </figure>
 +
                                </a>
  
                          
+
                       
 +
                            </div>
 +
                         </div>
  
 
                     </div>
 
                     </div>
Line 164: Line 188:
 
                             <div class="col-md-7 fix_p_l">
 
                             <div class="col-md-7 fix_p_l">
 
                                 <nav>
 
                                 <nav>
                                     <ul class="ft_bottom">    
+
                                     <ul class="ft_bottom">
 
                                         <li><a href="https://2016.igem.org/Team:Ionis_Paris">Home</a></li>
 
                                         <li><a href="https://2016.igem.org/Team:Ionis_Paris">Home</a></li>
                                         <li><a href="https://2016.igem.org/At_the_lab.html">At the Lab</a></li>
+
                                         <li><a href="https://2016.igem.org/Team:Ionis_Paris/Working_at_La_Paillasse">in the Lab</a></li>
                                         <li><a href="https://2016.igem.org/CelloCad"> Side Projects</a></li>
+
                                         <li><a href="https://2016.igem.org/Team:Ionis_Paris/Measurement"> Side Projects</a></li>
                                         <li><a href="Results">Results</a></li>
+
                                         <li><a href="https://2016.igem.org/Team:Ionis_Paris/Parts">Results</a></li>
                                         <li><a href="https://2016.igem.org/Team:Ionis_Paris/Human_Practice">Human Practice</a></li>
+
                                         <li><a href="https://2016.igem.org/Team:Ionis_Paris/HPintro">Human
                                         <li><a href="https://2016.igem.org/Team.html">Team</a></li>
+
                                            Practice</a></li>
 +
                                         <li><a href="https://2016.igem.org/Team:Ionis_Paris/Team">Team</a></li>
 
                                     </ul>
 
                                     </ul>
 
                                 </nav>
 
                                 </nav>
Line 176: Line 201:
 
                             <div class="col-md-5 fix_p">
 
                             <div class="col-md-5 fix_p">
 
                                 <div class="ft_paragraph">
 
                                 <div class="ft_paragraph">
                                     <p>©IONIS_IGEM_2016</a>.</p>  
+
                                     <p>©IONIS_IGEM_2016</a>.</p>
 
                                 </div>
 
                                 </div>
                             </div>
+
                             </div>
 
                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>
             </div>            
+
             </div>
        </footer>
+
    </footer>
        <!-- ====END FOOTER AREA==== -->
+
    <!-- ====END FOOTER AREA==== -->
     
+
        <!-- Nos scripts -->
+
<!-- TODO: Debug these scripts
+
        <script type="text/javascript" src="https://2016.igem.org/wiki/index.php?title=Template:IONIS_PARIS_JS_Venobox&action=raw&ctype=text/javascript"></script>
+
        <script type="text/javascript" src="https://2016.igem.org/wiki/index.php?title=Template:IONIS_PARIS_JS_MDB&action=raw&ctype=text/javascript"></script>
+
        <script type="text/javascript" src="https://2016.igem.org/wiki/index.php?title=Template:IONIS_PARIS_JS_Carousel&action=raw&ctype=text/javascript"></script>
+
-->
+
        <script type="text/javascript" src="https://2016.igem.org/wiki/index.php?title=Template:IONIS_PARIS_JS&action=raw&ctype=text/javascript"></script>
+
  
 +
    <!-- ====Google Maps API==== -->
 +
    <script src="https://maps.googleapis.com/maps/api/js"></script>
 +
 +
    <!-- Nos scripts -->
 +
    <script type="text/javascript" src="https://2016.igem.org/wiki/index.php?title=Template:IONIS_PARIS_JS&action=raw&ctype=text/javascript"></script>
  
     
 
 
</html>
 
</html>

Latest revision as of 21:03, 19 October 2016

Safe Project Design

Choosing a non-pathogenic chassis

For our project, we chose to use chassis lab strains of  E. coli bacteria (DH5a and BL21 strains). As non-pathogenic to humans, these E. coli strains belong to Risk Group 1 and thus require only basic precautions. We chose these organisms for convenience reasons: their genomes are entirely sequenced (we knew that the sigma factor required for our transcription factor biosensor was in the bacteria), they are easy to grow and safe to handle.

Choosing parts that will not harm humans/animals/plants

We designed our project in a way that it should not include parts that could be harmful to humans, animals or plants. Our project involves a XylR transcription factor and a luciferase reporter and neither of these parts are known to have safety issues.

Biological Containment

Our project is designed for a use in everyday life which is why we were particularly aware of safety issues. The main risks we have identified can be linked to the use of biological elements. We planned to use genetically engineered bacteria and therefore an ethical dimension had to be taken in account.
As those modified bacteria will be used in the field, our biggest challenge was to safely contain them. To adress this problem, we developed a containment method that will allow us to separate the bacteria from the environment. The container will be an enclosed device that allows air to be sampled and presented to the bacteria while preventing their accidental release (see Hardware). The first BioBrick tests were done in the laboratory under simulated environmental conditions. In the meantime, our Engineering team was developing a containment method similar to an airlock that would allow an efficient sampling of the air with no bacteria escaping the collection tube. However no field tests were made during the iGEM competition.
Although our biggest challenge comes from biology, we also identified a mechanical risk in the drone. There is the possibility that the drone will have an accident. We thought of a security system for this event that will allow us to handle such accident without harm and pollution (see Hardware).

Thinking about the future

We are also aware of the issues caused by the use of a drone in urban areas and the problems of selling our system to the general public. These issues motivated us to think of an economic model based on the sale of a service, not a product. In this way, our system will only be use by trained person belonging to our company and we will sell only data.