Difference between revisions of "Team:Ionis Paris/Description"

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{{Ionis_Paris}}
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{{IONIS_HEADER}}  
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        <link href='https://fonts.googleapis.com/css?family=Merriweather:400,700,700italic,400italic,300italic,300' rel='stylesheet' type='text/css'>
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        <link href='https://fonts.googleapis.com/css?family=Open+Sans:400,600,700,600italic,400italic,700italic,300,300italic' rel='stylesheet' type='text/css'>
  
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    <!-- Nos feuilles de style -->
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    <link rel="stylesheet" type="text/css" href="https://2016.igem.org/Template:IONIS_Paris-style-css-template?action=raw&ctype=text/css" />
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    <link rel="stylesheet" type="text/css" href="https://2016.igem.org/Template:IONIS_Paris-style-css?action=raw&ctype=text/css" />
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        <!-- ====BREADCUM START==== -->
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        <section class="blog_banner_project_genesis">
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                    <div class="col-sm-12">
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                        <div class="banner_title">
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                            <h1 id="back to the top">Project Description</h1>
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<div class="section_title">
  
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<div class="column full_size judges-will-not-evaluate">
 
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<h3>Quantifly, the future is now</h3>
 
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
 
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</div>
 
  
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                            <h2 class="secHd">Context</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>For our projet, we tried to focus on air pollution monitoring. We investigate the existing measurement techniques and found out that, among all the existing devices, none of them enable precise and versatile air pollution monitoring. Most of those measurement devices are placed on fixed measurement station and consequently they are unable to give precise results at a smaller scale. Atmospheric pollution quantification at small scale are more and more important due to the ever-increasing health concerns. Learn all the details of why we believe our project can be a real breakthrough in terms of air pollution measurement.</p>
  
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<div class="column full_size">
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<a href="https://2016.igem.org/Team:Ionis_Paris/Design" ><font color="DeepPink">Here</font></a>
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                        </div>
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                    </div>
  
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                    <div class="col-xs-12 col-sm-6">
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<div class="section_title">
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                            <h2 class="secHd">Our Core Project</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>We worked on a biosensor, a modified cell able to integrate a given signal and respond to it. Our goal was to create <i>E.coli </i> cells able to detect a specific pollutant and respond to it by emitting bioluminescence. We used the XylR protein, known to bind to certain aromatic compounds such as the toluene and benzene (two important pollutants). Once bound to those molecules, the XylR protein will form a tetramer and bind the Pu promoter. Pu promoter activation would then trigger luciferase synthesis and therefore bioluminescence production. </p>
  
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<h5>Quantifly, quantifying pollution with bioluminescence</h5>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Biology"><font color="DeepPink">The Biology behind Quantifly</font></a>
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<ul>
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<li>Detecting air pollution with an innovative biological system</li>
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<li>A multidisciplinary team gathering students from the IONIS Group schools</li>
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<li>Great communication about iGEM and its popularization</li>
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<li>Most of all, our passion for this project !</li>
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</ul>
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                        </div>
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                    </div>
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      </div>
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        <!-- ====ABOUT US==== -->
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<div class="section_title">
  
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<h3> Our project and its aspects </h3>
 
  
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<p> Our project consists in the alliance of biotechnology and engineering in order to design a new product. Therefore, we have several different components in our project </p>
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                            <h2 class="secHd">Measurement</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. We tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.</p>
  
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<div class="column half_size" >
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<a href="https://2016.igem.org/Team:Ionis_Paris/Measurement" ><font color="DeepPink">Learn more about our work with CelloCad</font></a>
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<div class = "highlight">
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                        </div>
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<h5> Biology </h5>
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                    </div>
  
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                    <div class="col-xs-12 col-sm-6">
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<div class="section_title">
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                            <h2 class="secHd">BioBricks</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>For our project, we developed a large number of BioBricks and characterized them though several processes (sequencing, luciferase assay). We improved two existing BioBricks: XylR Coding Sequence BioBrick (BBa_K1834844) by adding a His-tag allowing better characterization and Gluc protein (BBa_K1732027) though sequence optimization for use in <i>E.coli </i> and IDT synthesis. Please follow the link to access all informations about our:</p>
  
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<p> The core of our project is the creation and caracterisation of an innovative bacterial biosensor, able to efficiently quantify toluene through bioluminescence measurement </p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Parts"><font color="DeepPink">Parts & Characterization</font></a>
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</div>
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</br><a href="https://2016.igem.org/Team:Ionis_Paris/Proof"><font color="DeepPink">Proof of Concept</font></a>
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</div>
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                        </div>
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                    </div>
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      </div>
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<div class="column half_size" >
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        <!-- ====ABOUT US==== -->
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<div class = "highlight">
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<h5> Engineering </h5>
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<p> We are working in collaboration with engineering students in order to create a drone that will act as a mobile detection platform </p>
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<!-- ====ABOUT US==== -->
  
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            <div class="container">
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<h3> </h3>
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<div class="section_title">
  
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</div>
 
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<div class="column half_size" >
 
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<div class = "highlight">
 
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<h5> Computer Science and Bioinformatics </h5>
 
  
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<p> Our project features a wide bioinformatics dimension: two members of our team are computer scientists, and will be working on various domains such as drone controlling, web design, data analysis and game development </p>
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</div>
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                            <h2 class="secHd">Hardware</h2>
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</div>
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                        </div>
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                        <div class="about_text">
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                            <p>Our project aimed to solve the small-scale measurement problem and valorize the qualities of a biosensor for on-field measurement. Therefore, we built a drone able to perform on-field measurements and mappings, along with an airlock tube able to sample the air while preventing biosensor bacteria dissemination.</p>
  
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<div class="column half_size" >
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<a href="https://2016.igem.org/Team:Ionis_Paris/Hardware" ><font color="DeepPink">Learn more about our Hardware</font></a>
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<div class = "highlight">
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                        </div>
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<h5> Design </h5>
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                    </div>
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<p> We are working with designers in order to have the best wiki possible, along with material used to help everyone understand what we do throughout the whole competition </p>
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</div>
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                    <div class="col-xs-12 col-sm-6">
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</div>
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<div class="section_title">
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                            <h2 class="secHd">Entrepreneurship</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>We investigated the air pollution measurement market in order to establish a Business Model and find out application and future possible development of Quantifly. We also looked for informations about intellectual property to know what to do if our results were good enough to make a startup out of Quantifly.</p>
  
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<a href="https://2016.igem.org/Team:Ionis_Paris/Entrepreneurship"><font color="DeepPink">Learn more about the Entrepreunarial aspects of our project</font></a>
  
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                        </div>
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                    </div>
  
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                    <div class="col-xs-12 col-sm-6">
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<div class="section_title">
  
  
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                            <h2 class="secHd">Events</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>We organized and attended many events during this summer. Whether it was iGEMers Meetups, professional events, our team was very active! We enjoyed all of these events and definitely kept some very good memories of them. </br>
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We also participated in sports events that enabled us to raise some funds.</p>
  
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<a href="https://2016.igem.org/Team:Ionis_Paris/Events" ><font color="DeepPink">Learn more about our events</font></a>
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                        </div>
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                    </div>
  
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                    <div class="col-xs-12 col-sm-6">
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<div class="section_title">
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                            <h2 class="secHd">Collaborations</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>As our team was created last year, we wanted to meet other iGEM Teams and collaborate with them as much as we could. We worked with several iGEM teams from Paris to organize meet-up and to help in the lab experience. Our biggest collaboration was the organization of the European Experience.</p>
  
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<a href="https://2016.igem.org/Team:Ionis_Paris/Collaborations"><font color="DeepPink">Our collaborations</font></a>
  
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                        </div>
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</section>
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        <!-- ====ABOUT US==== -->
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    <!-- ====START SOCIAL Link==== -->
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    <div class="footer_social">
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        <div class="container-fluid">
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                <ul class="ft_top clearfix">
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                    <li class="waves-effect waves-light">
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                        <a href="https://www.facebook.com/ionisigem/?fref=ts" target="_blank">
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                            <i class="zmdi zmdi-facebook "></i>Facebook
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                        </a>
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                    </li>
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                    <li class="waves-effect waves-light">
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                        <a href="https://twitter.com/igem_ionis" target="_blank">
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                            <i class="zmdi zmdi-twitter"></i>Twitter
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                        </a>
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                    </li>
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                    <li class="waves-effect waves-light">
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                        <a href="https://www.youtube.com/channel/UC0RyQsB5YpweSRBYQlAdZDA" target="_blank">
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                            <i class="zmdi zmdi-youtube"></i>youtube
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                        </a>
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                    </li>
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                    <li class="waves-effect waves-light">
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                        <a href="https://www.linkedin.com/company/igem-ionis" target="_blank">
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                            <i class="zmdi zmdi-linkedin"></i>LinkedIn
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                        </a>
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                    </li>
  
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<div class="column full_size" >
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                </ul>
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            </div>
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        </div>
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    </div>
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    <!-- ====END FOOTER TOP==== -->
  
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<h5>Advice on writing your Project Description</h5>
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    <!-- ====START FOOTER AREA==== -->
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    <footer class="footer_area">
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        <div class="footer_middle">
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            <div class="container">
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                <div class="row">
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                    <div class="col-xs-12">
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                        <div class="scroll_area">
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                            <div class="sroll_top">
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                                <a href="#ancre"> <i class="zmdi zmdi-chevron-up btn waves-effect"> </i> </a>
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                            </div>
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                        </div>
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                <div class="row">
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                        <div class="col-lg-5 col-md-5 col-sm-12">
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                            <div class="middle_content">
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                                <h4>iGEM IONIS</h4>
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                                <p> We're a group of six different schools from the IONIS Education Group. For this
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                                    competition we wanted to take advantage of the multiple schools and fields of activity
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                                    given by the IONIS education group to create a solid project.</p>
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                                <a href="https://2016.igem.org/Team:Ionis_Paris/Team">Read More</a>
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                            </div>
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                        </div>
  
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<p>
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                        <div class="col-lg-3 col-lg-offset-1 col-md-4 col-sm-7">
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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                            <div class="footer_Widgets">
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</p>
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                                <h4>Stay in touch</h4>
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                                <div class="ft_contact">
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                                    <ul>
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                                        <li><i class="zmdi zmdi-pin"></i>
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                                            <span>Location: 66 Rue Guy Môquet, 94800 Villejuif, France</span>
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                                        </li>
  
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<p>
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                                        <li><i class="zmdi zmdi-email"></i>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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                                            <a href="mailto:igem.ionis@gmail.com">email: igem.ionis@gmail.com</a>
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</p>
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                                        </li>
  
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</div>
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                                    </ul>
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                                </div>
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                            </div>
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                        </div>
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                    <div class="col-lg-3 col-md-3 col-sm-5">
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                            <div class="footer_Widgets">
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                                <h4>Download the app</h4>
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                                <a href="https://itunes.apple.com/us/app/quantifly/id1166875690?ls=1&mt=8"
 +
                                  target="_blank" style="target-new: tab;">
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                                    <figure class="widget_gallery">
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                                        <div class="RXcircleEffect"></div>
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                                        <img src="https://static.igem.org/mediawiki/2016/8/8d/IONIS_IGEM_paris_logo_apple.png"
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                                            alt="" />
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                                        <figcaption>
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                                            <i class="zmdi zmdi-link"></i>
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                                        </figcaption>
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                                    </figure>
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                                </a>
 +
                                <a href="https://play.google.com/store/apps/details?id=fr.plnech.quantifly&hl=en"
 +
                                  target="_blank" style="target-new: tab;">
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                                    <figure class="widget_gallery">
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                                        <div class="RXcircleEffect"></div>
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                                        <img src="https://static.igem.org/mediawiki/2016/6/65/IONIS_IGEM_paris_google_play.png"
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                                            alt="" />
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                                        <figcaption>
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                                            <i class="zmdi zmdi-link"></i>
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                                        </figcaption>
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                                    </figure>
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                                </a>
  
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<div class="column half_size" >
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<h5>References</h5>
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                </div>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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                                    <p>©IONIS_IGEM_2016</a>.</p>
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Latest revision as of 21:06, 19 October 2016

Context

For our projet, we tried to focus on air pollution monitoring. We investigate the existing measurement techniques and found out that, among all the existing devices, none of them enable precise and versatile air pollution monitoring. Most of those measurement devices are placed on fixed measurement station and consequently they are unable to give precise results at a smaller scale. Atmospheric pollution quantification at small scale are more and more important due to the ever-increasing health concerns. Learn all the details of why we believe our project can be a real breakthrough in terms of air pollution measurement.

Here

Our Core Project

We worked on a biosensor, a modified cell able to integrate a given signal and respond to it. Our goal was to create E.coli cells able to detect a specific pollutant and respond to it by emitting bioluminescence. We used the XylR protein, known to bind to certain aromatic compounds such as the toluene and benzene (two important pollutants). Once bound to those molecules, the XylR protein will form a tetramer and bind the Pu promoter. Pu promoter activation would then trigger luciferase synthesis and therefore bioluminescence production.

The Biology behind Quantifly

Measurement

As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. We tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.

Learn more about our work with CelloCad

BioBricks

For our project, we developed a large number of BioBricks and characterized them though several processes (sequencing, luciferase assay). We improved two existing BioBricks: XylR Coding Sequence BioBrick (BBa_K1834844) by adding a His-tag allowing better characterization and Gluc protein (BBa_K1732027) though sequence optimization for use in E.coli and IDT synthesis. Please follow the link to access all informations about our:

Parts & Characterization
Proof of Concept

Hardware

Our project aimed to solve the small-scale measurement problem and valorize the qualities of a biosensor for on-field measurement. Therefore, we built a drone able to perform on-field measurements and mappings, along with an airlock tube able to sample the air while preventing biosensor bacteria dissemination.

Learn more about our Hardware

Entrepreneurship

We investigated the air pollution measurement market in order to establish a Business Model and find out application and future possible development of Quantifly. We also looked for informations about intellectual property to know what to do if our results were good enough to make a startup out of Quantifly.

Learn more about the Entrepreunarial aspects of our project

Events

We organized and attended many events during this summer. Whether it was iGEMers Meetups, professional events, our team was very active! We enjoyed all of these events and definitely kept some very good memories of them.
We also participated in sports events that enabled us to raise some funds.

Learn more about our events

Collaborations

As our team was created last year, we wanted to meet other iGEM Teams and collaborate with them as much as we could. We worked with several iGEM teams from Paris to organize meet-up and to help in the lab experience. Our biggest collaboration was the organization of the European Experience.

Our collaborations