Difference between revisions of "Team:Ionis Paris/Description"

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                             <h1>Project Description</h1>
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                             <h1 id="back to the top">Project Description</h1>
 
                              
 
                              
 
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<div class="section_title">
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                            <h2 class="secHd">Context</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>For our projet, we tried to focus on air pollution monitoring. We investigate the existing measurement techniques and found out that, among all the existing devices, none of them enable precise and versatile air pollution monitoring. Most of those measurement devices are placed on fixed measurement station and consequently they are unable to give precise results at a smaller scale. Atmospheric pollution quantification at small scale are more and more important due to the ever-increasing health concerns. Learn all the details of why we believe our project can be a real breakthrough in terms of air pollution measurement.</p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Design" ><font color="DeepPink">Here</font></a>
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<div class="section_title">
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                            <h2 class="secHd">Our Core Project</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>We worked on a biosensor, a modified cell able to integrate a given signal and respond to it. Our goal was to create <i>E.coli </i> cells able to detect a specific pollutant and respond to it by emitting bioluminescence. We used the XylR protein, known to bind to certain aromatic compounds such as the toluene and benzene (two important pollutants). Once bound to those molecules, the XylR protein will form a tetramer and bind the Pu promoter. Pu promoter activation would then trigger luciferase synthesis and therefore bioluminescence production. </p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Biology"><font color="DeepPink">The Biology behind Quantifly</font></a>
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<p>For our projet, we focused on air pollution. We had an in-depth reflexion about the existing measurement techniques, and found out that, among all the existing devices, none of them was satisfying. During our benchmark, we also realized that most of these devices were placed on fixed measurement station, and that they were by consequent unable to give precise results on a smaller scale, which are more and more needed du to the ever-increasing health concerns. Learn all the details of why we believe our project can be a real breakthrough in terms of air pollution measurement <a href="https://2016.igem.org/Team:Ionis_Paris/Design">here</a>
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                        <div class="blog_top">
 
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                                    <h4 class="blog_topHd">Our Core Project</h4>
 
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                            <h2 class="secHd">Measurement</h2>
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                        <div class="about_text">
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                            <p>As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. We tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.</p>
  
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<p>We started to work on a biosensor, a modified cell able to respond to a given signal. The goal of our cell would be to respond to the detection of a specific pollutant by emitting bioluminescence. We used the XylR protein, known to bind to molecules such as the toluene and benzene (two important pollutants), and once bound to activate the Pu promoter. Activation of this promoter would then trigger luciferase synthesis, allowing bioluminescence. Once our biosensor plasmid assembled, we used E. coli bacteria as a chassis organism.
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<a href="https://2016.igem.org/Team:Ionis_Paris/Measurement" ><font color="DeepPink">Learn more about our work with CelloCad</font></a>
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Click here to learn more about<a href ="https://2016.igem.org/Team:Ionis_Paris/Biology">the Biology behind Quantifly</a>.</p>
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                    <div class="col-xs-12 col-sm-6">
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                                    <h4 class="blog_topHd">Measurement</h4>
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<div class="section_title">
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                                </div>
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<p>As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. Thus, we tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid. Learn more about <a href ="https://2016.igem.org/Team:Ionis_Paris/Measurement">our work with CelloCad</a>.</p>
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                            <h2 class="secHd">BioBricks</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>For our project, we developed a large number of BioBricks and characterized them though several processes (sequencing, luciferase assay). We improved two existing BioBricks: XylR Coding Sequence BioBrick (BBa_K1834844) by adding a His-tag allowing better characterization and Gluc protein (BBa_K1732027) though sequence optimization for use in <i>E.coli </i> and IDT synthesis. Please follow the link to access all informations about our:</p>
  
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                        <div class="blog_top">
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<a href="https://2016.igem.org/Team:Ionis_Paris/Parts"><font color="DeepPink">Parts & Characterization</font></a>
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                                    <h4 class="blog_topHd">BioBricks</h4>
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</br><a href="https://2016.igem.org/Team:Ionis_Paris/Proof"><font color="DeepPink">Proof of Concept</font></a>
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<p>We developed a large number of BioBricks during our project, and tried to characterize them as best as we could, through several processes (e.g sequencing). We also worked on improving the characterization of the XylR Coding Sequence BioBrick (BBa_K1834844) through addition of a His-tag to it, and the sequence of the Gluc protein (BBa_K1732027). Please follow the link to access all informations about our <a href="https://2016.igem.org/Team:Ionis_Paris/Parts">Parts & Characterization</a>. We also managed to make our biosensor work, therefore realizing a first <a href ="https://2016.igem.org/Team:Ionis_Paris/Proof">Proof of Concept</a>.</p>
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                                    <h4 class="blog_topHd">Hardware</h4>
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<div class="section_title">
  
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<p>Our project aimed to solve the small-scale measurement problem and to valorize the qualities of a biosensor. We therefore developed a drone that could perform all the measurements and mappings we needed, along with an airlock tube able to sample the air while containing our biosensor bacteria. Learn more about our <a href="https://2016.igem.org/Team:Ionis_Paris/Hardware">Hardware.</p>
 
  
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                                    <h4 class="blog_topHd">Entrepreneurship</h4>
 
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<p>We investigated deeply what was the air pollution measurement market in our area, in order to establish a first Business Model for what we could make out of Quantifly. We also looked for informations concerning Intellectual Property, in order to know what to do if our results were good enough to make a startup out of Quantifly. Learn more about <a href="https://2016.igem.org/Team:Ionis_Paris/Entrepreneurship"the Entrepreunarial aspects of our project.
 
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                            <h2 class="secHd">Hardware</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>Our project aimed to solve the small-scale measurement problem and valorize the qualities of a biosensor for on-field measurement. Therefore, we built a drone able to perform on-field measurements and mappings, along with an airlock tube able to sample the air while preventing biosensor bacteria dissemination.</p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Hardware" ><font color="DeepPink">Learn more about our Hardware</font></a>
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                    <div class="col-xs-12 col-sm-6">
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<div class="section_title">
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                            <h2 class="secHd">Entrepreneurship</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>We investigated the air pollution measurement market in order to establish a Business Model and find out application and future possible development of Quantifly. We also looked for informations about intellectual property to know what to do if our results were good enough to make a startup out of Quantifly.</p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Entrepreneurship"><font color="DeepPink">Learn more about the Entrepreunarial aspects of our project</font></a>
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<div class="section_title">
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                            <h2 class="secHd">Events</h2>
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                        <div class="about_text">
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                            <p>We organized and attended many events during this summer. Whether it was iGEMers Meetups, professional events, our team was very active! We enjoyed all of these events and definitely kept some very good memories of them. </br>
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We also participated in sports events that enabled us to raise some funds.</p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Events" ><font color="DeepPink">Learn more about our events</font></a>
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<div class="section_title">
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                            <h2 class="secHd">Collaborations</h2>
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                        </div>
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                        <div class="about_text">
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                            <p>As our team was created last year, we wanted to meet other iGEM Teams and collaborate with them as much as we could. We worked with several iGEM teams from Paris to organize meet-up and to help in the lab experience. Our biggest collaboration was the organization of the European Experience.</p>
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<a href="https://2016.igem.org/Team:Ionis_Paris/Collaborations"><font color="DeepPink">Our collaborations</font></a>
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        <!-- ====ABOUT US==== -->
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    <!-- ====START SOCIAL Link==== -->
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                            <i class="zmdi zmdi-twitter"></i>Twitter
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                                <h4>iGEM IONIS</h4>
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                                <p> We're a group of six different schools from the IONIS Education Group. For this
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                                    competition we wanted to take advantage of the multiple schools and fields of activity
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                                    given by the IONIS education group to create a solid project.</p>
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                                <a href="https://2016.igem.org/Team:Ionis_Paris/Team">Read More</a>
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                            </div>
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                                <h4>Stay in touch</h4>
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                                            <span>Location: 66 Rue Guy Môquet, 94800 Villejuif, France</span>
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                                        </li>
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                                            <a href="mailto:igem.ionis@gmail.com">email: igem.ionis@gmail.com</a>
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Latest revision as of 21:06, 19 October 2016

Context

For our projet, we tried to focus on air pollution monitoring. We investigate the existing measurement techniques and found out that, among all the existing devices, none of them enable precise and versatile air pollution monitoring. Most of those measurement devices are placed on fixed measurement station and consequently they are unable to give precise results at a smaller scale. Atmospheric pollution quantification at small scale are more and more important due to the ever-increasing health concerns. Learn all the details of why we believe our project can be a real breakthrough in terms of air pollution measurement.

Here

Our Core Project

We worked on a biosensor, a modified cell able to integrate a given signal and respond to it. Our goal was to create E.coli cells able to detect a specific pollutant and respond to it by emitting bioluminescence. We used the XylR protein, known to bind to certain aromatic compounds such as the toluene and benzene (two important pollutants). Once bound to those molecules, the XylR protein will form a tetramer and bind the Pu promoter. Pu promoter activation would then trigger luciferase synthesis and therefore bioluminescence production.

The Biology behind Quantifly

Measurement

As we wanted to precisely quantify air pollution, we started working with CelloCad, a software used for plasmid optimization. We tried to improve the characterization of the two promoters of our biosensor, in order to build an optimized version of our plasmid.

Learn more about our work with CelloCad

BioBricks

For our project, we developed a large number of BioBricks and characterized them though several processes (sequencing, luciferase assay). We improved two existing BioBricks: XylR Coding Sequence BioBrick (BBa_K1834844) by adding a His-tag allowing better characterization and Gluc protein (BBa_K1732027) though sequence optimization for use in E.coli and IDT synthesis. Please follow the link to access all informations about our:

Parts & Characterization
Proof of Concept

Hardware

Our project aimed to solve the small-scale measurement problem and valorize the qualities of a biosensor for on-field measurement. Therefore, we built a drone able to perform on-field measurements and mappings, along with an airlock tube able to sample the air while preventing biosensor bacteria dissemination.

Learn more about our Hardware

Entrepreneurship

We investigated the air pollution measurement market in order to establish a Business Model and find out application and future possible development of Quantifly. We also looked for informations about intellectual property to know what to do if our results were good enough to make a startup out of Quantifly.

Learn more about the Entrepreunarial aspects of our project

Events

We organized and attended many events during this summer. Whether it was iGEMers Meetups, professional events, our team was very active! We enjoyed all of these events and definitely kept some very good memories of them.
We also participated in sports events that enabled us to raise some funds.

Learn more about our events

Collaborations

As our team was created last year, we wanted to meet other iGEM Teams and collaborate with them as much as we could. We worked with several iGEM teams from Paris to organize meet-up and to help in the lab experience. Our biggest collaboration was the organization of the European Experience.

Our collaborations