Difference between revisions of "Team:SDU-Denmark/Parts"

 
(124 intermediate revisions by 6 users not shown)
Line 14: Line 14:
 
     <div class="col-sm-11 col-sm-push-1" >
 
     <div class="col-sm-11 col-sm-push-1" >
 
<!----------CONTENT--------------------------------->
 
<!----------CONTENT--------------------------------->
−
<div class="column full_size">
 
  
 +
<img src="https://static.igem.org/mediawiki/2016/2/27/T--SDU-Denmark--Bricks_Line%404x.png" width="100%">
 +
<button class="accordion">Basic Parts</button>
 +
<div class="panel">
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018022" target="blank"><strong>BBa_K2018022</strong></a></p>
 +
<p>This BioBrick contains the coding region of <b>Phasin</b>, a protein derived from <em>R. eutropha</em>. Phasin binds to and reduces the size of PHA granules, including PHB. </p>
  
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018023" target="blank"><strong>BBa_K2018023</strong></a></p>
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
<p>BioBrick encodes a short <b>hemolysin tag</b>. This tag can be attached to other proteins for secretion through the hemolysin pathway.</p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018026" target="blank"><strong>BBa_K2018026</strong></a></p>
 +
<p>This BioBrick contains the native <b>RBS</b> for hemolysin B in  <em>R. eutropha</em>. The brick  also contains some native DNA prior to the RBS.<p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018027" target="blank"><strong>BBa_K2018027</strong></a></p>
 +
<p>This BioBrick contains the coding region of <b>hemolysin B</b>. Hemolysin B is an ABC transporter, that secretes the toxin hemolysin.<p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018028" target="blank"><strong>BBa_K2018028</strong></a></p>
 +
<p>This BioBrick contains the native <b>RBS</b> for hemolysin D in  <em>R. eutropha</em>. The brick  also contains some native DNA prior to the RBS.<p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018029" target="blank"><strong>BBa_K2018029</strong></a></p>
 +
<p>This BioBrick contains the coding region for <b>hemolysin D</b>, part of the inner membrane complex of the hemolysin type II secretion pathway.<p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018043" target="blank"><strong>BBa_K2018043</strong></a></p>
 +
<p>This BioBrick contains the sequence for the <b>MaSp1 CD gene</b>. This part is a monomer coding for the  dragline spider silk MaSp1 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part <a href="http://parts.igem.org/Part:BBa_K2018045" target="_blank">K2018045</a>, <a href="http://parts.igem.org/Part:K2018046" target="_blank">K2018046</a> and <a href="http://parts.igem.org/Part:BBa_K2018047" target="_blank">K2018047</a>.<p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018044" target="blank"><strong>BBa_K2018044</strong></a></p>
 +
<p>This BioBrick contains the sequence for the <b>MaSp2 CD gene</b>. This part is a monomer coding for the dragline spider silk MaSp2 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part <a href="http://parts.igem.org/Part:BBa_K2018045" target="_blank">K2018045</a>, <a href="http://parts.igem.org/Part:K2018046" target="_blank">K2018046</a> and <a href="http://parts.igem.org/Part:BBa_K2018047" target="_blank">K2018047</a>.<p>
 
</div>
 
</div>
  
 +
<button class="accordion">Composite Parts</button>
 +
<div class="panel">
 +
<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018010" target="blank"><strong>BBa_K2018010</strong></a></p>
 +
<p>This part is a functional BioBrick containing the coding region of <b>Laterosporulin</b>, with promoter, RBS and terminator. Laterosporulin is a bacteriocin produced by <em>Brevibacillus </em>sp. strain, and exhibit a broad spectrum of antibacterial activity against bacterias like: <em>B. subtilis , S. aureus, E. coli, P. aeruginosa, &nbsp;and L. monocytogenes.</em></p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018011" target="blank"><strong>BBa_K2018011</strong></a></p>
 +
<p>This BioBrick contains the coding region of, <b>ThuricinS</b>, a bacteriocin produced by <em>Bacillus thuringiensis</em>. ThuricinS targets a broad spectrum of pathogens, including <em>Pseudonoas aeruginosa and Enterobactoer Cloacae</em> which is often found in correlation to burn and wound infection.</p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018012" target="blank"><b>BBa_K2018012</b></a></p>
 +
<p>This BioBrick contains the coding region of <b>LacticinQ</b>, a bacteriocin produced by <em>Lactococcus lactis QU5</em> and has shown bactericidal activity against <em>Staphylococcus aureus</em> <span class="tooltip"><span class="tooltiptext"><a href=" http://www.ncbi.nlm.nih.gov/pubmed/22538663"target="blank"> Ma, Q., Yu, Z., Han, B., Wang, Q., & Zhang, R. (2012). Expression and Purification of Lacticin Q by Small Ubiquitin-Related Modifier Fusion in Escherichia coli. The Journal of Microbiology, 50(2), 326–331. http://doi.org/10.1007/s12275-012-1425-x.</a></span></span>. It functions by forming large toroidal pores by distributing membrane lipid organization.</p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018014" target="blank"><strong>BBa_K2018014</strong></a></p>
 +
<p>This BioBrick contains the coding region of <b>Laterosporulin-ThuricinS</b>, which is a hybrid bacteriocin with Laterosporulin and ThuricinS that we have designed.</p>
  
−
<div class="column half_size">
+
<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018015" target="blank"><strong>BBa_K2018015</strong></a></p>
−
<div class="highlight">
+
<p>This BioBrick contains the coding region of <b>LacticinQ-LacticinZ</b>, which is a hybrid bacteriocin with LacticinQ and LacticinZ that we have designed.</p>
−
<h5>Note</h5>
+
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
−
</div>
+
−
</div>
+
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018019" target="blank"><strong>BBa_K2018019</strong></a></p>
 +
<p>This BioBrick contains the coding region of <b>PyocinS5</b>, a bacteriocin produced by a specific strain of <em>P. aeruginosa</em> and elicit its effect against other strains of <em>P. aeruginosa</em>. Pyocin causes the cell membrane of target cells to be permeable and thereby causing leakage of intracellular materials, which cause cell death  <span class="tooltip"><span class="tooltiptext"><a href=" http://www.sciencedirect.com/science/article/pii/S0014579310005120"target="blank"> Ling, H., Saeidi, N., Haji Rasouliha, B., & Wook Chang, M. (2010). A predicted S-type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage. FEBS Letters, 584, 3354–3358. http://doi.org/10.1016/j.febslet.2010.06.021.</a></span></span>.</p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018024" target="blank"><strong>BBa_K2018024</strong></a></p>
 +
<p>This BioBrick contains the coding region of our biofused </b>phasin</b> with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (<a href="http://parts.igem.org/Part:BBa_K2018027" target="_blank">K2018027</a>) and hemolysin D (<a href="http://parts.igem.org/Part:BBa_K2018029" target="_blank">K2018029</a>). This BioBrick will simply bid to PHA granules in the cytoplasm and reduce the size of these.</p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018030" target="blank"><strong>BBa_K2018030</strong></a></p>
 +
<p>This part secretes PHB from PHB producing cells.<p>
  
−
<div class="column half_size">
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018048" target="blank"><strong>BBa_K2018048</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter combined with panK. It consists of <a href="http://parts.igem.org/Part:BBa_K2018036" target="blank">K2018036</a> and <a href="http://parts.igem.org/Part:BBa_K2018021" target="blank">K2018021</a>.</p>
  
−
<h5>Adding parts to the registry</h5>
 
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
−
</div>
 
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018049" target="blank"><strong>BBa_K2018049</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of <a href="http://parts.igem.org/Part:BBa_K2018036" target="blank">K2018036</a> and <a href="http://parts.igem.org/Part:BBa_K2018030" target="blank">K2018030</a>.</p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018050" target="blank"><strong>BBa_K2018050</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of <a href="http://parts.igem.org/Part:BBa_K2018036" target="blank">K2018036</a>, <a href="http://parts.igem.org/Part:BBa_K2018030" target="blank">K2018030</a> and  <a href="http://parts.igem.org/Part:BBa_K2018021" target="blank">K2018021</a>.</p>
  
  
  
−
<div class="column half_size">
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018033" target="blank"><strong>BBa_K2018033</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_K880005" target="_blank">K880005</a> and <a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">K934001</a> .<p>
  
−
<h5>What information do I need to start putting my parts on the Registry?</h5>
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018034" target="blank"><strong>BBa_K2018034</strong></a></p>
−
<p>The information needed to initially create a part on the Registry is:</p>
+
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_K081005" target="_blank">K081005</a> and <a href="http://parts.igem.org/Part:BBa_k934001" target="_blank">k934001</a>.<p>
−
<ul>
+
−
<li>Part Name</li>
+
−
<li>Part type</li>
+
−
<li>Creator</li>
+
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
  
−
<p>
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018035" target="blank"><strong>BBa_K2018035</strong></a></p>
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_K608004" target="_blank">K608004</a> and <a href="http://parts.igem.org/Part:BBa_k934001" target="_blank">k934001</a>.<p>
 +
 
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018036" target="blank"><strong>BBa_K2018036</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_K608003" target="_blank">K608003</a> and <a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">K934001</a>.<p>
 +
 
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018038" target="blank"><strong>BBa_K2018038</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_J23104" target="_blank">J23104</a> and <a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">K934001</a>.<p>
 +
 
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018039" target="blank"><strong>BBa_K2018039</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_J23106" target="_blank">J23106</a> and <a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">K934001</a>.<p>
  
 
</div>
 
</div>
  
  
−
<div class="column half_size">
+
<button class="accordion">Part Collection</button>
 +
<div id="foo" class="panel">
 +
<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;">We have created two part collections. The first one are our collection of bacteriocins, which all had been tested on different MRSA strains and <em> P. aeruginosa</em>
 +
The following BioBricks are a part of the collection. <a href="http://parts.igem.org/Part:BBa_K2018010" target="_blank">K2018010</a>, <a href="http://parts.igem.org/Part:BBa_K2018011" target="_blank">K2018011</a>, <a href="http://parts.igem.org/Part:BBa_K2018012" target="_blank">K2018012</a>, <a href="http://parts.igem.org/Part:BBa_K2018014" target="_blank">K2018014</a>, <a href="http://parts.igem.org/Part:BBa_K2018015" target="_blank">K2018015</a>, <a href="http://parts.igem.org/Part:BBa_K2018019" target="_blank">K2018019</a></p>
  
−
<h5>Inspiration</h5>
 
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"> We had also created a secretions system, which can secrete PHA, including PHB. The following BioBricks are a part of the collection. <a href="http://parts.igem.org/Part:BBa_K2018022" target="_blank">K2018022</a>, <a href="http://parts.igem.org/Part:BBa_K2018023" target="_blank">K2018023</a>, <a href="http://parts.igem.org/Part:BBa_K2018026" target="_blank">K2018026</a>, <a href="http://parts.igem.org/Part:BBa_K2018027" target="_blank">K2018027</a>, <a href="http://parts.igem.org/Part:BBa_K2018028" target="_blank">K2018028</a>, <a href="http://parts.igem.org/Part:BBa_K2018029" target="_blank">K2018029</a>, <a href="http://parts.igem.org/Part:BBa_K2018049" target="blank">K2018049</a> and <a href="http://parts.igem.org/Part:BBa_K2018050" target="blank">K2018050</a></p>
−
<ul>
+
 
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
−
</ul>
+
 
</div>
 
</div>
  
−
<div class="column full_size">
+
<button class="accordion">Existing BioBrick parts</button>
−
<h5>Part Table </h5>
+
<div id="foo" class="panel">
−
<div class="highlight">
+
  
 +
<p><a href=" http://parts.igem.org/wiki/index.php?title=Part%3ABBa_G00100" target="blank"><strong>VF2 Primer</strong></a></p>
 +
<p>This BioBrick contain standard iGEM primer <a href="http://parts.igem.org/wiki/index.php?title=Part%3ABBa_G00100" target="_blank">VF2</a></p>
 +
<p><a target="blank" href="http://parts.igem.org/wiki/index.php?title=Part%3ABBa_G00101target="blank"><strong>VR Primer</strong></a></p>
 +
<p>This BioBrick contain standard iGEM primer <a href="http://parts.igem.org/wiki/index.php?title=Part%3ABBa_G00101" target="_blank">VR</a></p>
  
−
</html>
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_J23104" target="_blank">BBa_ J23104</a></strong></p>
−
<groupparts>iGEM2016 Example</groupparts>
+
<p> The part is <a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"> J23104</a>, a strong promoter. </p>
−
<html>
+
−
</div>
+
−
</div>
+
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_J23106" target="_blank">BBa_ J23106</a></strong></p>
 +
<p> The part is <a href="http://parts.igem.org/Part:BBa_J23106" target="_blank">J23106</a>, a strong promoter. </p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K081005" target="_blank">BBa_ K081005</a></strong></p>
 +
<p> The part is composed of sub-parts <a href="http://parts.igem.org/Part:BBa_J23100" target="_blank"> J23100</a>, a strong promoter and <a href="http://parts.igem.org/Part:BBa_ B0030" target="_blank"> B0030</a>, a strong RBS. </p>
  
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><b><a href="http://parts.igem.org/Part:BBa_K1149051" target="_blank">BBa_K1149051</a></b></p>
 +
<p> This part code the phaCAB with hybrid promoter. The BioBrick is created by <a href="https://2013.igem.org/Team:Imperial_College/Biobricks" target="_blank"> Imperial College 2013 </a>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1692020" target="_blank">BBa_K1692020</a></strong></p>
 +
<p> This part code the <em>Staphylococcal</em> pantothenate kinase II, that increases the synthesis of coenzyme A and thereby increases PHB yield.The BioBrick is created by <a href="https://2015.igem.org/Team:Stanford-Brown/Parts" target="_blank"> Imperial College 2013 </a>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1763002" target="_blank">BBa_K1763002</a></strong></p>
 +
<p>This BioBrick we got from the <a title="UCLA" href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein, MaSp2. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 AB</a> and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp2. The brick is compatible with <a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/ICA_Oligo_Sequences" target="_blank">initiator</a> and part <a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">K1763003</a>.</p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><b><a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">BBa_K1763003</a></b></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 BC</a> and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763002" target="_blank">K1763002</a>, <a href="http://parts.igem.org/Part:BBa_K1763009" target="_blank">K1763009</a>&nbsp;and <a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">K1763004</a>.</p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><b><a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">BBa_K1763004</a></b></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 CA</a> and used in the ICA technique and is the third component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">K1763003</a>, <a href="http://parts.igem.org/Part:BBa_K1763002" target="_blank">K1763002</a> and <a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">K1763010</a>.</p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><b><a href="http://parts.igem.org/Part:BBa_K1763009" target="_blank">BBa_K1763009</a></b></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp2. A unique trade for this gene segment is that it codes for the same gene but is different because it contains a primer site. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 AB sequence core</a> and used in the ICA technique and is an alternative first component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">K1763003</a>, <a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">K1763004</a>, <a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a> and <a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/ICA_Oligo_Sequences" target="_blank">initiator</a>.</p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">BBa_K1763010</a></strong></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp1 AB</a> and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part&nbsp;</span><a href="http://parts.igem.org/Part:BBa_K1763011" target="_blank">K1763011</a>, <a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a> and <a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">K1763004</a></p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1763011" target="_blank">BBa_K1763011</a></strong></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp1 BC</a> and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">K1763010</a> and <a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a></p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">BBa_K1763012</a></strong></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp1 CA</a> and used in the ICA technique and is the thrid component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763011" target="_blank">K1763011</a>, <a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">K1763010</a>, <a href="http://parts.igem.org/Part:BBa_K1763002" target="_blank">K1763002</a>, and <a href="http://parts.igem.org/Part:BBa_K1763009" target="_blank">K1763009</a></p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K608003" target="_blank"> BBa_K608003</a></strong></p>
 +
<p> The part is composed of sub-parts <a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"> J23104</a>, a strong promoter and <a href="http://parts.igem.org/Part:BBa_ B0032" target="_blank"> B0032</a>, a medium RBS. </p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K608004" target="_blank"> BBa_K608004</a></strong></p>
 +
<p> The part is composed of sub-parts <a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"> J23104</a>, a strong promoter and <a href="http://parts.igem.org/Part:BBa_ B0031" target="_blank"> B0031</a>, a weak RBS. </p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K880005 " target="_blank">BBa_ K880005</a></strong></p>
 +
<p> The part is composed of sub-parts <a href="http://parts.igem.org/Part:BBa_J23100" target="_blank"> J23100</a>, a strong promoter and <a href="http://parts.igem.org/Part:BBa_ B0034" target="_blank"> B0034</a>, a strong RBS. </p>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">BBa_K9340011</a></strong></p>
 +
<p> This part code the phaCAB metabolic pathway that synthesises PHB from acetyl-CoA. The BioBrick is created by <a href="https://2012.igem.org/Team:Tokyo_Tech/Parts" target="_blank">Tokyo Tech 2012 </a>
 +
 +
</div>
 +
 +
<script>
 +
var acc = document.getElementsByClassName("accordion");
 +
var i;
 +
 +
for (i = 0; i < acc.length; i++) {
 +
    acc[i].onclick = function(){
 +
        this.classList.toggle("active");
 +
        this.nextElementSibling.classList.toggle("show");
 +
  }
 +
}
 +
</script>
 
<!--- END CONTENT------------------------------------>
 
<!--- END CONTENT------------------------------------>
 
</div> <!-- END COL-11 -->
 
</div> <!-- END COL-11 -->
Line 105: Line 193:
 
                 src="https://static.igem.org/mediawiki/2016/1/18/T--SDU-Denmark--twitter.png">
 
                 src="https://static.igem.org/mediawiki/2016/1/18/T--SDU-Denmark--twitter.png">
 
             </a>
 
             </a>
−
             <a class="pull-right" href="https://2016.igem.org/Team:SDU-Denmark/Basic_Part" src="#"><img id=nextpage src="https://static.igem.org/mediawiki/2016/f/fd/T--SDU-Denmark--nextarrow.png">
+
             <a class="pull-right" href="https://2016.igem.org/Team:SDU-Denmark/Notebook" src="#"><img id=nextpage src="https://static.igem.org/mediawiki/2016/f/fd/T--SDU-Denmark--nextarrow.png">
 
             </a>
 
             </a>
 
         </div>
 
         </div>
Line 114: Line 202:
 
<html>
 
<html>
 
     <style>
 
     <style>
−
       @media (min-width: 960px){
+
       @media (min-width: 992px){
−
             #parts{margin-left:-10%; padding-left:15%;width:110%;}
+
             #parts{margin-left:-10%; padding-left:15%;width:105%;}
 
         }
 
         }
 
     </style>
 
     </style>
Line 129: Line 217:
 
//Hide the other panels
 
//Hide the other panels
 
$(".sidesubmenu").not(sidesubmenu).hide();
 
$(".sidesubmenu").not(sidesubmenu).hide();
 +
 +
        flag = $('#accordion').find('#4');
 +
 +
//Expand or collapse this panel
 +
topsubmenu = $(flag).find('.topsubmenu');
 +
topsubmenu.show();
 +
 +
 +
 +
//Hide the other panels
 +
$(".topsubmenu").not(topsubmenu).hide();
 
 
 
 

Latest revision as of 21:41, 19 October 2016

Parts

BBa_K2018022

This BioBrick contains the coding region of Phasin, a protein derived from R. eutropha. Phasin binds to and reduces the size of PHA granules, including PHB.

BBa_K2018023

BioBrick encodes a short hemolysin tag. This tag can be attached to other proteins for secretion through the hemolysin pathway.

BBa_K2018026

This BioBrick contains the native RBS for hemolysin B in R. eutropha. The brick also contains some native DNA prior to the RBS.

BBa_K2018027

This BioBrick contains the coding region of hemolysin B. Hemolysin B is an ABC transporter, that secretes the toxin hemolysin.

BBa_K2018028

This BioBrick contains the native RBS for hemolysin D in R. eutropha. The brick also contains some native DNA prior to the RBS.

BBa_K2018029

This BioBrick contains the coding region for hemolysin D, part of the inner membrane complex of the hemolysin type II secretion pathway.

BBa_K2018043

This BioBrick contains the sequence for the MaSp1 CD gene. This part is a monomer coding for the dragline spider silk MaSp1 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part K2018045, K2018046 and K2018047.

BBa_K2018044

This BioBrick contains the sequence for the MaSp2 CD gene. This part is a monomer coding for the dragline spider silk MaSp2 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part K2018045, K2018046 and K2018047.

BBa_K2018010

This part is a functional BioBrick containing the coding region of Laterosporulin, with promoter, RBS and terminator. Laterosporulin is a bacteriocin produced by Brevibacillus sp. strain, and exhibit a broad spectrum of antibacterial activity against bacterias like: B. subtilis , S. aureus, E. coli, P. aeruginosa,  and L. monocytogenes.

BBa_K2018011

This BioBrick contains the coding region of, ThuricinS, a bacteriocin produced by Bacillus thuringiensis. ThuricinS targets a broad spectrum of pathogens, including Pseudonoas aeruginosa and Enterobactoer Cloacae which is often found in correlation to burn and wound infection.

BBa_K2018012

This BioBrick contains the coding region of LacticinQ, a bacteriocin produced by Lactococcus lactis QU5 and has shown bactericidal activity against Staphylococcus aureus Ma, Q., Yu, Z., Han, B., Wang, Q., & Zhang, R. (2012). Expression and Purification of Lacticin Q by Small Ubiquitin-Related Modifier Fusion in Escherichia coli. The Journal of Microbiology, 50(2), 326–331. http://doi.org/10.1007/s12275-012-1425-x.. It functions by forming large toroidal pores by distributing membrane lipid organization.

BBa_K2018014

This BioBrick contains the coding region of Laterosporulin-ThuricinS, which is a hybrid bacteriocin with Laterosporulin and ThuricinS that we have designed.

BBa_K2018015

This BioBrick contains the coding region of LacticinQ-LacticinZ, which is a hybrid bacteriocin with LacticinQ and LacticinZ that we have designed.

BBa_K2018019

This BioBrick contains the coding region of PyocinS5, a bacteriocin produced by a specific strain of P. aeruginosa and elicit its effect against other strains of P. aeruginosa. Pyocin causes the cell membrane of target cells to be permeable and thereby causing leakage of intracellular materials, which cause cell death Ling, H., Saeidi, N., Haji Rasouliha, B., & Wook Chang, M. (2010). A predicted S-type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage. FEBS Letters, 584, 3354–3358. http://doi.org/10.1016/j.febslet.2010.06.021..

BBa_K2018024

This BioBrick contains the coding region of our biofused phasin with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (K2018027) and hemolysin D (K2018029). This BioBrick will simply bid to PHA granules in the cytoplasm and reduce the size of these.

BBa_K2018030

This part secretes PHB from PHB producing cells.

BBa_K2018048

This part is phaCAB with a hybrid promoter combined with panK. It consists of K2018036 and K2018021.

BBa_K2018049

This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of K2018036 and K2018030.

BBa_K2018050

This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of K2018036, K2018030 and K2018021.

BBa_K2018033

This part is phaCAB with a hybrid promoter. It consists of K880005 and K934001 .

BBa_K2018034

This part is phaCAB with a hybrid promoter. It consists of K081005 and k934001.

BBa_K2018035

This part is phaCAB with a hybrid promoter. It consists of K608004 and k934001.

BBa_K2018036

This part is phaCAB with a hybrid promoter. It consists of K608003 and K934001.

BBa_K2018038

This part is phaCAB with a hybrid promoter. It consists of J23104 and K934001.

BBa_K2018039

This part is phaCAB with a hybrid promoter. It consists of J23106 and K934001.

We have created two part collections. The first one are our collection of bacteriocins, which all had been tested on different MRSA strains and P. aeruginosa The following BioBricks are a part of the collection. K2018010, K2018011, K2018012, K2018014, K2018015, K2018019

We had also created a secretions system, which can secrete PHA, including PHB. The following BioBricks are a part of the collection. K2018022, K2018023, K2018026, K2018027, K2018028, K2018029, K2018049 and K2018050

VF2 Primer

This BioBrick contain standard iGEM primer VF2

VR Primer

This BioBrick contain standard iGEM primer VR

BBa_ J23104

The part is J23104, a strong promoter.

BBa_ J23106

The part is J23106, a strong promoter.

BBa_ K081005

The part is composed of sub-parts J23100, a strong promoter and B0030, a strong RBS.

BBa_K1149051

This part code the phaCAB with hybrid promoter. The BioBrick is created by Imperial College 2013

BBa_K1692020

This part code the Staphylococcal pantothenate kinase II, that increases the synthesis of coenzyme A and thereby increases PHB yield.The BioBrick is created by Imperial College 2013

BBa_K1763002

This BioBrick we got from the University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein, MaSp2. This part is called MaSp2 AB and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp2. The brick is compatible with initiator and part K1763003.

BBa_K1763003

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called MaSp2 BC and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763002, K1763009 and K1763004.

BBa_K1763004

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called MaSp2 CA and used in the ICA technique and is the third component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763003, K1763002 and K1763010.

BBa_K1763009

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. A unique trade for this gene segment is that it codes for the same gene but is different because it contains a primer site. This part is called MaSp2 AB sequence core and used in the ICA technique and is an alternative first component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763003, K1763004, K1763012 and initiator.

BBa_K1763010

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 AB and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763011, K1763012 and K1763004

BBa_K1763011

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 BC and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763010 and K1763012

BBa_K1763012

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 CA and used in the ICA technique and is the thrid component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763011, K1763010, K1763002, and K1763009

BBa_K608003

The part is composed of sub-parts J23104, a strong promoter and B0032, a medium RBS.

BBa_K608004

The part is composed of sub-parts J23104, a strong promoter and B0031, a weak RBS.

BBa_ K880005

The part is composed of sub-parts J23100, a strong promoter and B0034, a strong RBS.

BBa_K9340011

This part code the phaCAB metabolic pathway that synthesises PHB from acetyl-CoA. The BioBrick is created by Tokyo Tech 2012