Difference between revisions of "Team:SDU-Denmark/Parts"

 
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.accordion div.pane {
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<img src="https://static.igem.org/mediawiki/2016/2/27/T--SDU-Denmark--Bricks_Line%404x.png" width="100%">
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<button class="accordion">Basic Parts</button>
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<div class="panel">
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018022" target="blank"><strong>BBa_K2018022</strong></a></p>
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<p>This BioBrick contains the coding region of <b>Phasin</b>, a protein derived from <em>R. eutropha</em>. Phasin binds to and reduces the size of PHA granules, including PHB. </p>
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018023" target="blank"><strong>BBa_K2018023</strong></a></p>
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<p>BioBrick encodes a short <b>hemolysin tag</b>. This tag can be attached to other proteins for secretion through the hemolysin pathway.</p>
  
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018026" target="blank"><strong>BBa_K2018026</strong></a></p>
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<p>This BioBrick contains the native <b>RBS</b> for hemolysin B in  <em>R. eutropha</em>. The brick  also contains some native DNA prior to the RBS.<p>
  
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</style>
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018027" target="blank"><strong>BBa_K2018027</strong></a></p>
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<p>This BioBrick contains the coding region of <b>hemolysin B</b>. Hemolysin B is an ABC transporter, that secretes the toxin hemolysin.<p>
  
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<div class="accordion" style="width:650px;">
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018028" target="blank"><strong>BBa_K2018028</strong></a></p>
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<p>This BioBrick contains the native <b>RBS</b> for hemolysin D in  <em>R. eutropha</em>. The brick  also contains some native DNA prior to the RBS.<p>
  
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  <div class="accordionTitel">Coding</div>
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018029" target="blank"><strong>BBa_K2018029</strong></a></p>
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  <div class="pane" >
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<p>This BioBrick contains the coding region for <b>hemolysin D</b>, part of the inner membrane complex of the hemolysin type II secretion pathway.<p>
  
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<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018043" target="blank"><strong>BBa_K2018043</strong></a></p>
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<p>This BioBrick contains the sequence for the <b>MaSp1 CD gene</b>. This part is a monomer coding for the  dragline spider silk MaSp1 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part <a href="http://parts.igem.org/Part:BBa_K2018045" target="_blank">K2018045</a>, <a href="http://parts.igem.org/Part:K2018046" target="_blank">K2018046</a> and <a href="http://parts.igem.org/Part:BBa_K2018047" target="_blank">K2018047</a>.<p>
  
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088000">BBa_K1088000</a></span><br>
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<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018044" target="blank"><strong>BBa_K2018044</strong></a></p>
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The BioBrick contains the coding region of <span class="intro">the <span class="specialWord">dxs</span> gene</span> derived from the Gram-positive bacteria <span class="specialWord">Bacillus subtilis</span>. Dxs is the first enzyme in the MEP pathway converting pyruvate and <span class="tooltipLink">GAP</span> <span class="tooltip"><span class="tooltipHeader">GAP</span>Glyceraldehyde-3-phosphate</span> into <span class="tooltipLink">DXP.</span> <span class="tooltip"><span class="tooltipHeader">DXP</span>1-Deoxy-D-xylulose 5-phosphate</span> Has been sequenced.
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<p>This BioBrick contains the sequence for the <b>MaSp2 CD gene</b>. This part is a monomer coding for the dragline spider silk MaSp2 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part <a href="http://parts.igem.org/Part:BBa_K2018045" target="_blank">K2018045</a>, <a href="http://parts.igem.org/Part:K2018046" target="_blank">K2018046</a> and <a href="http://parts.igem.org/Part:BBa_K2018047" target="_blank">K2018047</a>.<p>
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<br><br>
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</div>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088003">BBa_K1088003</a></span><br>
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The BioBrick contains the coding region of <span class="intro">the <span class="specialWord">HRT2</span> gene</span> derived from <span class="specialWord">Hevea brasiliensis</span> and codon-optimized for <span class="specialWord">Escherichia coli</span>. HRT2 is the prenyl transferase that polymerizes <span class="tooltipLink">IPP</span> <span class="tooltip"><span class="tooltipHeader">IPP</span>Isopentenyl pyrophosphate</span> and <span class="tooltipLink">DMAPP</span> <span class="tooltip"><span class="tooltipHeader">DMAPP</span>Dimethylallyl pyrophosphate</span> into rubber. Has been sequenced and HRT2 function characterized by H<sup>1</sup>-NMR.
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<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088004">BBa_K1088004</a></span><br>
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The BioBrick contains the coding region of <span class="intro">the <span class="specialWord">ispG</span> gene</span> derived from the Gram-negative bacteria <span class="specialWord">Escherichia coli</span>. ispG is the sixth enzyme in the MEP pathway converting <span class="tooltipLink">MEcPP</span> <span class="tooltip"><span class="tooltipHeader">MEcPP</span>2-C-methyl-D-erythritol 2,4-cyclopyrophosphate</span> into <span class="tooltipLink">HMB-PP.</span> <span class="tooltip"><span class="tooltipHeader">HMB-PP</span>(E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate</span> Has been sequenced.
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<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088005">BBa_K1088005</a></span><br>
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The BioBrick contains the coding region of <span class="intro">the <span class="specialWord">araC</span> gene</span> derived from the Gram-negative bacteria <span class="specialWord">Escherichia coli</span>. AraC is a DNA-binding protein that regulates the transcription of operons involved in arabinose metabolism. With glucose present AraC functions as a repressor, and without glucose and with arabinose present it functions as an activator.
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<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088018">BBa_K1088018</a></span><br>
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The BioBrick contains the coding region of <span class="intro">the <span class="specialWord">lacI</span> gene</span> derived from the Gram-negative bacteria <span class="specialWord">Escherichia coli</span>. LacI is a DNA-binding protein that inhibits the transcription from the lac promoter when allolactose or <span class="tooltipLink">IPTG</span> <span class="tooltip"><span class="tooltipHeader">IPTG</span>Isopropyl β-D-1-thiogalactopyranoside</span> is absent. Has been sequenced and LacI function characterized by <span class="tooltipLink">FACS.</span> <span class="tooltip"><span class="tooltipHeader">FACS</span>Fluorescence Activated Cell Sorting</span> and growth experiment.
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<br>
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  </div>
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<button class="accordion">Composite Parts</button>
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<div class="panel">
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<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018010" target="blank"><strong>BBa_K2018010</strong></a></p>
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<p>This part is a functional BioBrick containing the coding region of <b>Laterosporulin</b>, with promoter, RBS and terminator. Laterosporulin is a bacteriocin produced by <em>Brevibacillus </em>sp. strain, and exhibit a broad spectrum of antibacterial activity against bacterias like: <em>B. subtilis , S. aureus, E. coli, P. aeruginosa, &nbsp;and L. monocytogenes.</em></p>
  
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<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018011" target="blank"><strong>BBa_K2018011</strong></a></p>
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<p>This BioBrick contains the coding region of, <b>ThuricinS</b>, a bacteriocin produced by <em>Bacillus thuringiensis</em>. ThuricinS targets a broad spectrum of pathogens, including <em>Pseudonoas aeruginosa and Enterobactoer Cloacae</em> which is often found in correlation to burn and wound infection.</p>
  
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<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018012" target="blank"><b>BBa_K2018012</b></a></p>
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<p>This BioBrick contains the coding region of <b>LacticinQ</b>, a bacteriocin produced by <em>Lactococcus lactis QU5</em> and has shown bactericidal activity against <em>Staphylococcus aureus</em> <span class="tooltip"><span class="tooltiptext"><a href=" http://www.ncbi.nlm.nih.gov/pubmed/22538663"target="blank"> Ma, Q., Yu, Z., Han, B., Wang, Q., & Zhang, R. (2012). Expression and Purification of Lacticin Q by Small Ubiquitin-Related Modifier Fusion in Escherichia coli. The Journal of Microbiology, 50(2), 326–331. http://doi.org/10.1007/s12275-012-1425-x.</a></span></span>. It functions by forming large toroidal pores by distributing membrane lipid organization.</p>
  
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  <div class="accordionTitel">Regulatory devices</div>
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<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018014" target="blank"><strong>BBa_K2018014</strong></a></p>
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  <div class="pane" >
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<p>This BioBrick contains the coding region of <b>Laterosporulin-ThuricinS</b>, which is a hybrid bacteriocin with Laterosporulin and ThuricinS that we have designed.</p>
  
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088017">BBa_K1088017</a></span><br>
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<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018015" target="blank"><strong>BBa_K2018015</strong></a></p>
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<span class="intro">Pcon-araC-term:</span> <span class="specialWord">araC</span> is being expressed from a constitutively active promoter. A terminator is put behind the coding region to prevent transcription of genes downstream of the activator. The device was used to check if we could enhance the control of the arabinose promoter. Has been sequenced and AraC function characterized by northern blot.
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<p>This BioBrick contains the coding region of <b>LacticinQ-LacticinZ</b>, which is a hybrid bacteriocin with LacticinQ and LacticinZ that we have designed.</p>
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<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088019">BBa_K1088019</a></span><br>
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<span class="intro">Pcon-lacI(N)-term:</span> <span class="specialWord">lacI</span> is being expressed from a constitutively active promoter. A terminator is put behind the coding region to prevent transcription of genes downstream of the repressor. The device was used to enable us to control the lactose promoter. This device proved be most effective together for expression control. LacI(N) function characterized by GFP fusion using <span class="tooltipLink">FACS.</span> <span class="tooltip"><span class="tooltipHeader">FACS</span>Fluorescence Activated Cell Sorting</span> and growth experiment.
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<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088020">BBa_K1088020</a></span><br>
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<span class="intro">Pcon-lacI:LVA-term:</span> <span class="specialWord">lacI:LVA</span> (<a class="dialogLink" href="http://parts.igem.org/Part:Ba_C0012">BBa_C0012</a>) is being expressed from a constitutively active promoter. A terminator is put behind the coding region to prevent transcription of genes downstream of the repressor. The LVA-tag is a tag for degradation, and thus there is increased turnover of the protein. The device is meant to enable us to control the lactose promoter. However natural LacI proved to be more effective than the LVA-tagged. Has been sequenced and LacI:LVA function characterized by GFP fusion using FACS and growth experiment.
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<br>
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<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018019" target="blank"><strong>BBa_K2018019</strong></a></p>
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<p>This BioBrick contains the coding region of <b>PyocinS5</b>, a bacteriocin produced by a specific strain of <em>P. aeruginosa</em> and elicit its effect against other strains of <em>P. aeruginosa</em>. Pyocin causes the cell membrane of target cells to be permeable and thereby causing leakage of intracellular materials, which cause cell death  <span class="tooltip"><span class="tooltiptext"><a href=" http://www.sciencedirect.com/science/article/pii/S0014579310005120"target="blank"> Ling, H., Saeidi, N., Haji Rasouliha, B., & Wook Chang, M. (2010). A predicted S-type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage. FEBS Letters, 584, 3354–3358. http://doi.org/10.1016/j.febslet.2010.06.021.</a></span></span>.</p>
  
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  </div>
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018024" target="blank"><strong>BBa_K2018024</strong></a></p>
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<p>This BioBrick contains the coding region of our biofused </b>phasin</b> with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (<a href="http://parts.igem.org/Part:BBa_K2018027" target="_blank">K2018027</a>) and hemolysin D (<a href="http://parts.igem.org/Part:BBa_K2018029" target="_blank">K2018029</a>). This BioBrick will simply bid to PHA granules in the cytoplasm and reduce the size of these.</p>
  
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018030" target="blank"><strong>BBa_K2018030</strong></a></p>
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<p>This part secretes PHB from PHB producing cells.<p>
  
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018048" target="blank"><strong>BBa_K2018048</strong></a></p>
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<p>This part is phaCAB with a hybrid promoter combined with panK. It consists of <a href="http://parts.igem.org/Part:BBa_K2018036" target="blank">K2018036</a> and <a href="http://parts.igem.org/Part:BBa_K2018021" target="blank">K2018021</a>.</p>
  
  
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  <div class="accordionTitel">Reporter fusions</div>
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018049" target="blank"><strong>BBa_K2018049</strong></a></p>
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  <div class="pane">
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<p>This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of <a href="http://parts.igem.org/Part:BBa_K2018036" target="blank">K2018036</a> and <a href="http://parts.igem.org/Part:BBa_K2018030" target="blank">K2018030</a>.</p>
  
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088006">BBa_K1088006</a></span><br>
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018050" target="blank"><strong>BBa_K2018050</strong></a></p>
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<span class="intro">Pcon-<span class="specialWord">dxs (B. subtilis)</span>-amilCP</span> expresses the <span class="specialWord">dxs</span> gene derived from  <span class="specialWord">Bacillus subtilis</span> linked to GFP, and is under the control of the lactose promoter. AmilCP proved to be a poor fusion protein for the Dxs protein. Has been sequenced.
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<p>This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of <a href="http://parts.igem.org/Part:BBa_K2018036" target="blank">K2018036</a>, <a href="http://parts.igem.org/Part:BBa_K2018030" target="blank">K2018030</a> and <a href="http://parts.igem.org/Part:BBa_K2018021" target="blank">K2018021</a>.</p>
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<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088007">BBa_K1088007</a></span><br>
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<span class="intro">Plac-<span class="specialWord">dxs (E. coli)</span>-GFP</span> expresses the <span class="specialWord">dxs</span> gene derived from  <span class="specialWord">Escherichia coli</span> linked to GFP, and is under the control of the lactose promoter. The device was used to check the expression level of the <span class="specialWord">E. coli dxs</span> gene under various conditions. Has been sequenced.
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<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088008">BBa_K1088008</a></span><br>
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−
<span class="intro">Plac-<span class="specialWord">dxs (B. subtilis)</span>:GFP</span> expresses the <span class="specialWord">dxs</span> gene derived from <span class="specialWord">Bacillus subtilis</span> linked to GFP, and is under the control of the lactose promoter. The device is was used to check the expression level of the <span class="specialWord">B.subtilis dxs</span> gene under various conditions. Has been sequenced and Plac function characterized by GFP fusion using <span class="tooltipLink">FACS.</span> <span class="tooltip"><span class="tooltipHeader">FACS</span>Fluorescence Activated Cell Sorting</span> and growth experiment.
+
−
<br><br>
+
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088009">BBa_K1088009</a></span><br>
+
−
<span class="intro">Pcon-<span class="specialWord">lacI:LVA</span>-Plac-<span class="specialWord">dxs (B. subtilis)</span>-GFP</span> expresses the <span class="specialWord">dxs</span> gene derived from  <span class="specialWord">B. subtilis</span> linked to GFP, and is under the control of the lactose promoter. The device to check the expression level of the  <span class="specialWord">Bacillus subtilis</span> dxs gene under various conditions. Our LacI:LVA (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088020">BBa_K1088020</a>) device (with a constitutive promoter was added to optimize the expression control through the lactose promoter. Natural LacI proved to be more efficient, though. Has been sequenced. LacI and Plac function characterized by GFP fusion using FACS and growth experiment.
+
−
<br><br>
+
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088010">BBa_K1088010</a></span><br>
+
−
<span class="intro">Pcon-<span class="specialWord">lacI:LVA</span>-term-Plac-<span class="specialWord">dxs (E. coli)</span>-GFP</span> expresses the <span class="specialWord">dxs</span> gene derived from <span class="specialWord">Escherichia coli</span> linked to GFP, and is under the control of the lactose promoter. The device is meant for us to check the expression level of the <span class="specialWord">dxs</span> gene under various conditions. Our LacI:LVA (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088020">BBa_K1088020</a>) device (with a constitutive promoter was added to optimize the expression control through the lactose promoter.
+
−
<br><br>
+
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088026">BBa_K1088026</a></span><br>
+
−
<span class="intro">Pcon-<span class="specialWord">lacI(N)</span>-Plac-<span class="specialWord">dxs (B. subtilis)</span>-GFP</span> expresses the <span class="specialWord">dxs</span> gene derived from  <span class="specialWord">Bacillus subtilis</span> linked to GFP, and is under the control of the lactose promoter. The device is meant for us to check the expression level of the dxs gene under various conditions. Furthermore the <span class="specialWord">lacI</span> gene with a constitutive promoter has been added to optimize the expression control through the lactose promoter. This device proved to have the most efficient expression control (see results for more detail). Has been sequenced. LacI and Plac function characterized by GFP fusion using FACS and growth experiment.
+
−
<br>
+
  
−
  </div>
 
  
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018033" target="blank"><strong>BBa_K2018033</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_K880005" target="_blank">K880005</a> and <a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">K934001</a> .<p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018034" target="blank"><strong>BBa_K2018034</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_K081005" target="_blank">K081005</a> and <a href="http://parts.igem.org/Part:BBa_k934001" target="_blank">k934001</a>.<p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018035" target="blank"><strong>BBa_K2018035</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_K608004" target="_blank">K608004</a> and <a href="http://parts.igem.org/Part:BBa_k934001" target="_blank">k934001</a>.<p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018036" target="blank"><strong>BBa_K2018036</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_K608003" target="_blank">K608003</a> and <a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">K934001</a>.<p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018038" target="blank"><strong>BBa_K2018038</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_J23104" target="_blank">J23104</a> and <a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">K934001</a>.<p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><a href="http://parts.igem.org/Part:BBa_K2018039" target="blank"><strong>BBa_K2018039</strong></a></p>
 +
<p>This part is phaCAB with a hybrid promoter. It consists of <a href="http://parts.igem.org/Part:BBa_J23106" target="_blank">J23106</a> and <a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">K934001</a>.<p>
  
−
  <div class="accordionTitel">Constitutively active production devices</div>
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</div>
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  <div class="pane">
+
  
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088011">BBa_K1088011</a></span><br>
 
−
<span class="intro">Plac-<span class="specialWord">dxs (B. subtilis)</span></span> expresses the <span class="specialWord">dxs</span> gene derived from  <span class="specialWord">B. subtilis</span>, and is under the control of the lactose promoter. The device is meant for us to increase the amount of IPP and DMAPP in the cell. Plac function characterized by GFP fusion using <span class="tooltipLink">FACS.</span> <span class="tooltip"><span class="tooltipHeader">FACS</span>Fluorescence Activated Cell Sorting</span> and growth experiment.
 
−
<br><br>
 
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088012">BBa_K1088012</a></span><br>
 
−
<span class="intro">Plac-<span class="specialWord">dxs (E. coli)</span></span> expresses the <span class="specialWord">dxs</span> gene (<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K118000" title="">BBa_K118000</a>) derived from  <span class="specialWord">E. coli</span>, and is under the control of the lactose promoter. The device is meant for us to increase the amount of IPP and DMAPP in the cell. Has been sequenced.
 
−
<br>
 
  
−
  </div>
+
<button class="accordion">Part Collection</button>
 +
<div id="foo" class="panel">
 +
<p><img src="https://static.igem.org/mediawiki/2016/e/e9/T--SDU-Denmark--minibacto.png" height="20%" style="float:right;margin-left:15px;">We have created two part collections. The first one are our collection of bacteriocins, which all had been tested on different MRSA strains and <em> P. aeruginosa</em>
 +
The following BioBricks are a part of the collection. <a href="http://parts.igem.org/Part:BBa_K2018010" target="_blank">K2018010</a>, <a href="http://parts.igem.org/Part:BBa_K2018011" target="_blank">K2018011</a>, <a href="http://parts.igem.org/Part:BBa_K2018012" target="_blank">K2018012</a>, <a href="http://parts.igem.org/Part:BBa_K2018014" target="_blank">K2018014</a>, <a href="http://parts.igem.org/Part:BBa_K2018015" target="_blank">K2018015</a>, <a href="http://parts.igem.org/Part:BBa_K2018019" target="_blank">K2018019</a></p>
  
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"> We had also created a secretions system, which can secrete PHA, including PHB. The following BioBricks are a part of the collection. <a href="http://parts.igem.org/Part:BBa_K2018022" target="_blank">K2018022</a>, <a href="http://parts.igem.org/Part:BBa_K2018023" target="_blank">K2018023</a>, <a href="http://parts.igem.org/Part:BBa_K2018026" target="_blank">K2018026</a>, <a href="http://parts.igem.org/Part:BBa_K2018027" target="_blank">K2018027</a>, <a href="http://parts.igem.org/Part:BBa_K2018028" target="_blank">K2018028</a>, <a href="http://parts.igem.org/Part:BBa_K2018029" target="_blank">K2018029</a>, <a href="http://parts.igem.org/Part:BBa_K2018049" target="blank">K2018049</a> and <a href="http://parts.igem.org/Part:BBa_K2018050" target="blank">K2018050</a></p>
  
 +
</div>
  
−
  <div class="accordionTitel">Regulable production devices</div>
+
<button class="accordion">Existing BioBrick parts</button>
−
  <div class="pane current">
+
<div id="foo" class="panel">
  
 +
<p><a href=" http://parts.igem.org/wiki/index.php?title=Part%3ABBa_G00100" target="blank"><strong>VF2 Primer</strong></a></p>
 +
<p>This BioBrick contain standard iGEM primer <a href="http://parts.igem.org/wiki/index.php?title=Part%3ABBa_G00100" target="_blank">VF2</a></p>
 +
<p><a target="blank" href="http://parts.igem.org/wiki/index.php?title=Part%3ABBa_G00101target="blank"><strong>VR Primer</strong></a></p>
 +
<p>This BioBrick contain standard iGEM primer <a href="http://parts.igem.org/wiki/index.php?title=Part%3ABBa_G00101" target="_blank">VR</a></p>
  
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088013">BBa_K1088013</a></span><br>
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_J23104" target="_blank">BBa_ J23104</a></strong></p>
−
<span class="intro">Pcon-<span class="specialWord">lacI:LVA</span>-term-Plac-<span class="specialWord">dxs (B. subtilis)</span></span>  expresses the dxs gene derived from  <span class="specialWord">B. subtilis</span>, and is under the control of the lactose promoter. The device is meant for us to increase the amount of <span class="tooltipLink">IPP</span> <span class="tooltip"><span class="tooltipHeader">IPP</span>Isopentenyl pyrophosphate</span> and <span class="tooltipLink">DMAPP</span> <span class="tooltip"><span class="tooltipHeader">DMAPP</span>Dimethylallyl pyrophosphate</span> in the cell. Furthermore the <span class="specialWord">lacI:LVA</span> gene with a constitutive promoter was added to optimize the expression control through the lactose promoter. Natural LacI proved to be more efficient, though. Has been sequenced.
+
<p> The part is <a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"> J23104</a>, a strong promoter. </p>
−
<br><br>
+
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088014">BBa_K1088014</a></span><br>
+
−
<span class="intro">Pcon-<span class="specialWord">lacI:LVA</span>-term-Plac-<span class="specialWord">dxs (E. coli)</span></span> expresses the <span class="specialWord">dxs</span> gene derived from  <span class="specialWord">E. coli</span>, and is under the control of the lactose promoter. The device is meant for us to increase the amount of IPP and DMAPP in the cell. Furthermore the <span class="specialWord">lacI-LVA</span> gene with a constitutive promoter has been added to optimize the expression control through the lactose promoter.
+
−
<br><br>
+
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088015">BBa_K1088015</a></span><br>
+
−
<span class="intro">Pcon-<span class="specialWord">lacI:LVA</span>-term-Plac-<span class="specialWord">dxs (B. subtilis)</span>-ispG</span> expresses the <span class="specialWord">dxs</span> gene derived from  <span class="specialWord">B. subtilis</span>, and is under the control of the lactose promoter. The device was build to increase the amount of IPP and DMAPP in the cell if the first rate limiting step was overcome.  Furthermore the <span class="specialWord">lacI:LVA</span> gene with a constitutive promoter has been added to optimize the expression control through the lactose promoter (see results for description LacI-LVA efficiency).
+
−
<br><br>
+
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088016">BBa_K1088016</a></span><br>
+
−
<span class="intro">Pcon-<span class="specialWord">araC</span>-term-Para-<span class="specialWord">HRT2-(3xFLAG)</span></span> expresses the <span class="specialWord">HRT2</span> gene derived from <span class="specialWord">Hevea brasiliensis</span>, and is under the control of the arabinose promoter. The device was made to enable the bacteria to polymerize IPP and DMAPP into rubber. Furthermore the arabinose promoter regulator AraC has been added to check if it would enhance the expression control of arabinose promoter. It did not seem to improve expression control. Has been sequenced. AraC and Para function characterized by Northern blot and HRT2 function characterized by H<sup>1</sup>-NMR.
+
−
<br><br>
+
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088024">BBa_K1088024</a></span><br>
+
−
<span class="intro">Para-<span class="specialWord">HRT2-(3xFLAG)</span></span> expresses the <span class="specialWord">HRT2</span> gene derived from <span class="specialWord">Hevea brasiliensis</span>, and is under the control of the arabinose promoter. The device is meant to enable the bacteria to polymerize IPP and DMAPP into rubber. Has been sequenced. AraC and Para function characterized by Northern blot and HRT2 function characterized by H<sup>1</sup>-NMR.
+
−
<br><br>
+
−
<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088027">BBa_K1088027</a></span><br>
+
−
<span class="intro">Pcon-<span class="specialWord">lacI(N)</span>-Plac-<span class="specialWord">dxs (B. subtilis)</span></span> expresses the <span class="specialWord">dxs</span> gene derived from  <span class="specialWord">B. subtilis</span>, and is under the control of the lactose promoter. The device is meant for us to increase the amount of IPP and DMAPP in the cell. Furthermore the <span class="specialWord">lacI</span> gene with a constitutive promoter was added to optimize the expression control through the lactose promoter. This addition proved to have the most efficient expression control. LacI and Plac function characterized by GFP fusion using <span class="tooltipLink">FACS.</span> <span class="tooltip"><span class="tooltipHeader">FACS</span>Fluorescence Activated Cell Sorting</span> and growth experiment. 
+
  
−
<br>
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_J23106" target="_blank">BBa_ J23106</a></strong></p>
 +
<p> The part is <a href="http://parts.igem.org/Part:BBa_J23106" target="_blank">J23106</a>, a strong promoter. </p>
  
−
  </div>
+
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K081005" target="_blank">BBa_ K081005</a></strong></p>
 +
<p> The part is composed of sub-parts <a href="http://parts.igem.org/Part:BBa_J23100" target="_blank"> J23100</a>, a strong promoter and <a href="http://parts.igem.org/Part:BBa_ B0030" target="_blank"> B0030</a>, a strong RBS. </p>
  
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><b><a href="http://parts.igem.org/Part:BBa_K1149051" target="_blank">BBa_K1149051</a></b></p>
 +
<p> This part code the phaCAB with hybrid promoter. The BioBrick is created by <a href="https://2013.igem.org/Team:Imperial_College/Biobricks" target="_blank"> Imperial College 2013 </a>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1692020" target="_blank">BBa_K1692020</a></strong></p>
 +
<p> This part code the <em>Staphylococcal</em> pantothenate kinase II, that increases the synthesis of coenzyme A and thereby increases PHB yield.The BioBrick is created by <a href="https://2015.igem.org/Team:Stanford-Brown/Parts" target="_blank"> Imperial College 2013 </a>
 +
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1763002" target="_blank">BBa_K1763002</a></strong></p>
 +
<p>This BioBrick we got from the <a title="UCLA" href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein, MaSp2. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 AB</a> and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp2. The brick is compatible with <a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/ICA_Oligo_Sequences" target="_blank">initiator</a> and part <a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">K1763003</a>.</p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><b><a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">BBa_K1763003</a></b></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 BC</a> and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763002" target="_blank">K1763002</a>, <a href="http://parts.igem.org/Part:BBa_K1763009" target="_blank">K1763009</a>&nbsp;and <a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">K1763004</a>.</p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><b><a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">BBa_K1763004</a></b></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 CA</a> and used in the ICA technique and is the third component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">K1763003</a>, <a href="http://parts.igem.org/Part:BBa_K1763002" target="_blank">K1763002</a> and <a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">K1763010</a>.</p>
  
−
</div>
+
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><b><a href="http://parts.igem.org/Part:BBa_K1763009" target="_blank">BBa_K1763009</a></b></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp2. A unique trade for this gene segment is that it codes for the same gene but is different because it contains a primer site. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 AB sequence core</a> and used in the ICA technique and is an alternative first component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">K1763003</a>, <a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">K1763004</a>, <a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a> and <a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/ICA_Oligo_Sequences" target="_blank">initiator</a>.</p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">BBa_K1763010</a></strong></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp1 AB</a> and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part&nbsp;</span><a href="http://parts.igem.org/Part:BBa_K1763011" target="_blank">K1763011</a>, <a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a> and <a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">K1763004</a></p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1763011" target="_blank">BBa_K1763011</a></strong></p>
 +
<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp1 BC</a> and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">K1763010</a> and <a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a></p>
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<p><img src="https://static.igem.org/mediawiki/2016/4/45/T--SDU-Denmark--minisilk.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">BBa_K1763012</a></strong></p>
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<p>This BioBrick we got from <a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a>. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called <a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp1 CA</a> and used in the ICA technique and is the thrid component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part <a href="http://parts.igem.org/Part:BBa_K1763011" target="_blank">K1763011</a>, <a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">K1763010</a>, <a href="http://parts.igem.org/Part:BBa_K1763002" target="_blank">K1763002</a>, and <a href="http://parts.igem.org/Part:BBa_K1763009" target="_blank">K1763009</a></p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K608003" target="_blank"> BBa_K608003</a></strong></p>
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<p> The part is composed of sub-parts <a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"> J23104</a>, a strong promoter and <a href="http://parts.igem.org/Part:BBa_ B0032" target="_blank"> B0032</a>, a medium RBS. </p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K608004" target="_blank"> BBa_K608004</a></strong></p>
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<p> The part is composed of sub-parts <a href="http://parts.igem.org/Part:BBa_J23104" target="_blank"> J23104</a>, a strong promoter and <a href="http://parts.igem.org/Part:BBa_ B0031" target="_blank"> B0031</a>, a weak RBS. </p>
  
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<h3><strong>Basic</strong></h3>
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<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K880005 " target="_blank">BBa_ K880005</a></strong></p>
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<p> The part is composed of sub-parts <a href="http://parts.igem.org/Part:BBa_J23100" target="_blank"> J23100</a>, a strong promoter and <a href="http://parts.igem.org/Part:BBa_ B0034" target="_blank"> B0034</a>, a strong RBS. </p>
  
 +
<p><img src="https://static.igem.org/mediawiki/2016/8/87/T--SDU-Denmark--miniPHB.png" height="20%" style="float:right;margin-left:15px;"><strong><a href="http://parts.igem.org/Part:BBa_K934001" target="_blank">BBa_K9340011</a></strong></p>
 +
<p> This part code the phaCAB metabolic pathway that synthesises PHB from acetyl-CoA. The BioBrick is created by <a href="https://2012.igem.org/Team:Tokyo_Tech/Parts" target="_blank">Tokyo Tech 2012 </a>
  
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<h3><strong>Composite</strong></h3>
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<p><a href="http://parts.igem.org/Part:BBa_K2018010" target="blank"><strong>BBa_K2018010</strong></a></p>
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<p><span style="font-weight: 400;">This part is a functional BioBrick contain the coding region of </span><strong>Laterosporulin, </strong><span style="font-weight: 400;">, with promoter, RBS and terminator. Laterosporulin is a bacteriocin produced by </span><em><span style="font-weight: 400;">Brevibacillus </span></em><span style="font-weight: 400;">sp. strain, and exhibit a broad spectrum of antibacterial activity against bacterias like:</span><em><span style="font-weight: 400;"> B. subtilis , S. aureus, E. coli, P. aeruginosa, &nbsp;and L. monocytogenes.</span></em><span style="font-weight: 400;"></span></p>
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<p><a href="http://parts.igem.org/Part:BBa_K2018011" target="blank"><strong>BBa_K2018011</strong></a></p>
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<p><span style="font-weight: 400;">This BioBrick contain the coding region of, </span><strong>Thuricin S</strong><span style="font-weight: 400;">, a bacteriocin produced by </span><em><span style="font-weight: 400;">Bacillus thuringiensis. </span></em><span style="font-weight: 400;">Thuricin S target a broad spectrum of pathogens, including </span><em><span style="font-weight: 400;">Pseudonoas aeruginosa and Enterobactoer Cloacae</span></em><span style="font-weight: 400;"> which is often found in correlation to burn and wound infection. </span></p>
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<p><a href="http://parts.igem.org/Part:BBa_K2018012" target="blank"><strong>BBa_K2018012</strong></a></p>
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<p><span style="font-weight: 400;">This BioBrick contain the coding region of </span><strong>Lacticin Q</strong><span style="font-weight: 400;">, a bacteriocin </span><span style="font-weight: 400;">&nbsp;produced by </span><em><span style="font-weight: 400;">Lactococcus lactis QU5 </span></em><span style="font-weight: 400;">&nbsp;and has shown bactericidal activity against </span><em><span style="font-weight: 400;">Staphylococcus aureus </span></em><span style="font-weight: 400;">[</span><a href="http://www.ncbi.nlm.nih.gov/pubmed/22538663" target="blank"><span style="font-weight: 400;">http://www.ncbi.nlm.nih.gov/pubmed/22538663</span></a><span style="font-weight: 400;">]. It function by forming large toroidal pores by distributing membrane lipid organization.</span></p>
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<p><a href="http://parts.igem.org/Part:BBa_K2018014" target="blank"><strong>BBa_K2018014</strong></a></p>
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<p><span style="font-weight: 400;">This BioBrick contain the coding region of </span><strong>Laterosporulin-Thuricin S, </strong><span style="font-weight: 400;">which is a hybrid bacteriocin with &nbsp;Laterosporulin and Thuricin S we had designed. &nbsp;</span></p>
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<p><a href="http://parts.igem.org/Part:BBa_K2018015" target="blank"><strong>BBa_K2018015</strong></a></p>
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<p><span style="font-weight: 400;">This BioBrick contain the coding region of </span><strong>Lacticin Q-Lacticin Z, </strong><span style="font-weight: 400;">which is a hybrid bacteriocin with Lacticin Q and Lacticin Z we had designed. &nbsp;</span></p>
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<p><a href="http://parts.igem.org/Part:BBa_K2018019" target="blank"><strong>BBa_K2018019</strong></a></p>
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<p><span style="font-weight: 400;">This BioBrick contain the coding region of </span><strong>Pyocin S5, </strong><span style="font-weight: 400;">a bacteriocin</span> <span style="font-weight: 400;">produced by &nbsp;a specific strain of </span><em><span style="font-weight: 400;">P. aeruginosa </span></em><span style="font-weight: 400;">and elicit its effect against other strains of </span><em><span style="font-weight: 400;">P. aeruginosa</span></em><span style="font-weight: 400;">. Pyocin causes the cell membrane of target cells to be permeable and thereby causing leakage of intracellular materials, which cause cell death [</span><a href="http://www.sciencedirect.com/science/article/pii/S0014579310005120" target="blank"><span style="font-weight: 400;">http://www.sciencedirect.com/science/article/pii/S0014579310005120</span></a><span style="font-weight: 400;">].</span></p>
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<p><a href="http://parts.igem.org/Part:BBa_K2018024" target="blank"><strong>BBa_K2018024</strong></a></p>
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<p><span style="font-weight: 400;">This BioBrick contains the coding region of our biofused </span><strong>phasin </strong><span style="font-weight: 400;">with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (K2018027) and hemolysin D (K2018029). This BioBrick will simply bid to PHA granules in the cytoplasm &nbsp;and reduce the size of these.</span></p>
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Latest revision as of 21:41, 19 October 2016

Parts

BBa_K2018022

This BioBrick contains the coding region of Phasin, a protein derived from R. eutropha. Phasin binds to and reduces the size of PHA granules, including PHB.

BBa_K2018023

BioBrick encodes a short hemolysin tag. This tag can be attached to other proteins for secretion through the hemolysin pathway.

BBa_K2018026

This BioBrick contains the native RBS for hemolysin B in R. eutropha. The brick also contains some native DNA prior to the RBS.

BBa_K2018027

This BioBrick contains the coding region of hemolysin B. Hemolysin B is an ABC transporter, that secretes the toxin hemolysin.

BBa_K2018028

This BioBrick contains the native RBS for hemolysin D in R. eutropha. The brick also contains some native DNA prior to the RBS.

BBa_K2018029

This BioBrick contains the coding region for hemolysin D, part of the inner membrane complex of the hemolysin type II secretion pathway.

BBa_K2018043

This BioBrick contains the sequence for the MaSp1 CD gene. This part is a monomer coding for the dragline spider silk MaSp1 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part K2018045, K2018046 and K2018047.

BBa_K2018044

This BioBrick contains the sequence for the MaSp2 CD gene. This part is a monomer coding for the dragline spider silk MaSp2 and is used for the technique called ICA. This specific part contains specific overhangs, which have sticky ends compatible with e.g. part K2018045, K2018046 and K2018047.

BBa_K2018010

This part is a functional BioBrick containing the coding region of Laterosporulin, with promoter, RBS and terminator. Laterosporulin is a bacteriocin produced by Brevibacillus sp. strain, and exhibit a broad spectrum of antibacterial activity against bacterias like: B. subtilis , S. aureus, E. coli, P. aeruginosa,  and L. monocytogenes.

BBa_K2018011

This BioBrick contains the coding region of, ThuricinS, a bacteriocin produced by Bacillus thuringiensis. ThuricinS targets a broad spectrum of pathogens, including Pseudonoas aeruginosa and Enterobactoer Cloacae which is often found in correlation to burn and wound infection.

BBa_K2018012

This BioBrick contains the coding region of LacticinQ, a bacteriocin produced by Lactococcus lactis QU5 and has shown bactericidal activity against Staphylococcus aureus Ma, Q., Yu, Z., Han, B., Wang, Q., & Zhang, R. (2012). Expression and Purification of Lacticin Q by Small Ubiquitin-Related Modifier Fusion in Escherichia coli. The Journal of Microbiology, 50(2), 326–331. http://doi.org/10.1007/s12275-012-1425-x.. It functions by forming large toroidal pores by distributing membrane lipid organization.

BBa_K2018014

This BioBrick contains the coding region of Laterosporulin-ThuricinS, which is a hybrid bacteriocin with Laterosporulin and ThuricinS that we have designed.

BBa_K2018015

This BioBrick contains the coding region of LacticinQ-LacticinZ, which is a hybrid bacteriocin with LacticinQ and LacticinZ that we have designed.

BBa_K2018019

This BioBrick contains the coding region of PyocinS5, a bacteriocin produced by a specific strain of P. aeruginosa and elicit its effect against other strains of P. aeruginosa. Pyocin causes the cell membrane of target cells to be permeable and thereby causing leakage of intracellular materials, which cause cell death Ling, H., Saeidi, N., Haji Rasouliha, B., & Wook Chang, M. (2010). A predicted S-type pyocin shows a bactericidal activity against clinical Pseudomonas aeruginosa isolates through membrane damage. FEBS Letters, 584, 3354–3358. http://doi.org/10.1016/j.febslet.2010.06.021..

BBa_K2018024

This BioBrick contains the coding region of our biofused phasin with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (K2018027) and hemolysin D (K2018029). This BioBrick will simply bid to PHA granules in the cytoplasm and reduce the size of these.

BBa_K2018030

This part secretes PHB from PHB producing cells.

BBa_K2018048

This part is phaCAB with a hybrid promoter combined with panK. It consists of K2018036 and K2018021.

BBa_K2018049

This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of K2018036 and K2018030.

BBa_K2018050

This part is phaCAB with a hybrid promoter combined with our secretion system. It consists of K2018036, K2018030 and K2018021.

BBa_K2018033

This part is phaCAB with a hybrid promoter. It consists of K880005 and K934001 .

BBa_K2018034

This part is phaCAB with a hybrid promoter. It consists of K081005 and k934001.

BBa_K2018035

This part is phaCAB with a hybrid promoter. It consists of K608004 and k934001.

BBa_K2018036

This part is phaCAB with a hybrid promoter. It consists of K608003 and K934001.

BBa_K2018038

This part is phaCAB with a hybrid promoter. It consists of J23104 and K934001.

BBa_K2018039

This part is phaCAB with a hybrid promoter. It consists of J23106 and K934001.

We have created two part collections. The first one are our collection of bacteriocins, which all had been tested on different MRSA strains and P. aeruginosa The following BioBricks are a part of the collection. K2018010, K2018011, K2018012, K2018014, K2018015, K2018019

We had also created a secretions system, which can secrete PHA, including PHB. The following BioBricks are a part of the collection. K2018022, K2018023, K2018026, K2018027, K2018028, K2018029, K2018049 and K2018050

VF2 Primer

This BioBrick contain standard iGEM primer VF2

VR Primer

This BioBrick contain standard iGEM primer VR

BBa_ J23104

The part is J23104, a strong promoter.

BBa_ J23106

The part is J23106, a strong promoter.

BBa_ K081005

The part is composed of sub-parts J23100, a strong promoter and B0030, a strong RBS.

BBa_K1149051

This part code the phaCAB with hybrid promoter. The BioBrick is created by Imperial College 2013

BBa_K1692020

This part code the Staphylococcal pantothenate kinase II, that increases the synthesis of coenzyme A and thereby increases PHB yield.The BioBrick is created by Imperial College 2013

BBa_K1763002

This BioBrick we got from the University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein, MaSp2. This part is called MaSp2 AB and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp2. The brick is compatible with initiator and part K1763003.

BBa_K1763003

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called MaSp2 BC and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763002, K1763009 and K1763004.

BBa_K1763004

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called MaSp2 CA and used in the ICA technique and is the third component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763003, K1763002 and K1763010.

BBa_K1763009

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. A unique trade for this gene segment is that it codes for the same gene but is different because it contains a primer site. This part is called MaSp2 AB sequence core and used in the ICA technique and is an alternative first component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763003, K1763004, K1763012 and initiator.

BBa_K1763010

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 AB and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763011, K1763012 and K1763004

BBa_K1763011

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 BC and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763010 and K1763012

BBa_K1763012

This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 CA and used in the ICA technique and is the thrid component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763011, K1763010, K1763002, and K1763009

BBa_K608003

The part is composed of sub-parts J23104, a strong promoter and B0032, a medium RBS.

BBa_K608004

The part is composed of sub-parts J23104, a strong promoter and B0031, a weak RBS.

BBa_ K880005

The part is composed of sub-parts J23100, a strong promoter and B0034, a strong RBS.

BBa_K9340011

This part code the phaCAB metabolic pathway that synthesises PHB from acetyl-CoA. The BioBrick is created by Tokyo Tech 2012