Difference between revisions of "Team:SDU-Denmark/Project"

 
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<p>Our project face the growing problem of plastic pollution and the evolution of antibiotic resistant bacteria. Bacto-Aid is a sustainable concept with bacteria aiding us. We focused on producing a bandage, consisting of recombinant spider silk integrated with antimicrobial peptides, making it preventive towards infections. The plastic, of which we wish to attach the spider silk to, is biodegradable and produced in the lab.<br></p><br><br><br>
  
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In this chapter you will be taken through the details of the Bacto-Aid project with an thorough description of the project and the motivation behind it. Then more detailed insight into the experiments of the project and last but not least the results of those experiments.
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<p class="icon1" style="text-align:center;">Bacteriocins</p><br>
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<p>In order to realize our project we set out to; 1) test the antimicrobial effects of different bacteriocins on pathogenic strains of bacteria, 2) assemble a silk expressing gene-construct, and 3) optimize the PHB production. Additionally, we tried to create a hybrid silk gene-construct in which bacteriocins are incorporated in between the silk monomers.</p><br>
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<li>The bacteriocins were found to be more efficient towards <i>S. aureus</i> than traditional antibiotics. Furthermore, we designed our own hybrid bacteriocins and proved a synergistic effect was achieved by doing so.</li><br>
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<li>We worked hard in order to produce a silk construct we could purify. However, we discovered too late that our inconsistent results were probably due to expired streptavidin beads (which are crucial for the silk assembly method).</li><br>
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<li>We managed to optimize the production of PHB. The incorporation of a secretion system into the plastic production, enabled us to synthesize vast amounts of PHB. Some of the produced plastic was used for 3D printing a piece of a jaw to prove the usefulness of our biodegradable plastic.</li></ul><br>
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<p>Scientific reproduction has been a cornerstone of our project from the start since we have been using other iGEM projects as basis for the development of our Bacto-Aid. We have likewise focussed on reproducibility of our own project in order to assure that the good ideas lives on. </p><br>
  
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<p>WHO estimates that antimicrobial resistance will cause 10 million premature deaths by the year 2050, exceeding cancer related deaths. The amount of antibiotics used worldwide creates a selective pressure on bacteria to evolve and share counter measures towards antibiotics, eventually rendering us defenseless even against simple infections. Our solution focuses on infections that occur in wounds which today are treated with band-aids and antibiotic therapy.</p>
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<p>We are working on a preventive band-aid consisting of recombinant spider silk with integrated antimicrobial peptides, thereby reducing the need for conventional antibiotics. We use spider silk due to its angiogenetic properties and proliferative effect on keratinocytes. The silk with the antimicrobial peptides will be attached to the plastic polymer, poly-β-hydroxy butyrate (PHB), with which our entire construct becomes biodegradable and immune-neutral. Our project, Bacto-Aid, faces the growing problems of plastic pollution and evolution of resistant bacteria.</p>
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Latest revision as of 21:59, 19 October 2016

Project

Our project face the growing problem of plastic pollution and the evolution of antibiotic resistant bacteria. Bacto-Aid is a sustainable concept with bacteria aiding us. We focused on producing a bandage, consisting of recombinant spider silk integrated with antimicrobial peptides, making it preventive towards infections. The plastic, of which we wish to attach the spider silk to, is biodegradable and produced in the lab.




Bacteriocins


Spider silk


poly-β-hydroxybutyrate PHB


In order to realize our project we set out to; 1) test the antimicrobial effects of different bacteriocins on pathogenic strains of bacteria, 2) assemble a silk expressing gene-construct, and 3) optimize the PHB production. Additionally, we tried to create a hybrid silk gene-construct in which bacteriocins are incorporated in between the silk monomers.


  • The bacteriocins were found to be more efficient towards S. aureus than traditional antibiotics. Furthermore, we designed our own hybrid bacteriocins and proved a synergistic effect was achieved by doing so.

  • We worked hard in order to produce a silk construct we could purify. However, we discovered too late that our inconsistent results were probably due to expired streptavidin beads (which are crucial for the silk assembly method).

  • We managed to optimize the production of PHB. The incorporation of a secretion system into the plastic production, enabled us to synthesize vast amounts of PHB. Some of the produced plastic was used for 3D printing a piece of a jaw to prove the usefulness of our biodegradable plastic.

Scientific reproduction has been a cornerstone of our project from the start since we have been using other iGEM projects as basis for the development of our Bacto-Aid. We have likewise focussed on reproducibility of our own project in order to assure that the good ideas lives on.


So let’s get down to the details.