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</style> | </style> | ||
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+ | <h1>Plate Reader Results</h1> | ||
+ | <br><br> | ||
<h3>Absorbance Values at 600 nm</h3> | <h3>Absorbance Values at 600 nm</h3> | ||
<figure> | <figure> | ||
− | <IMG SRC="https://static.igem.org/mediawiki/2016/f/fb/T--Newcastle--Abs600.png" ALT="some text" WIDTH= | + | <IMG SRC="https://static.igem.org/mediawiki/2016/f/fb/T--Newcastle--Abs600.png" ALT="some text" WIDTH=800 HEIGHT=600> |
<p><figcaption>Figure 1. Recorded OD600 values as noted in the provided Interlab Excel data sheet </figcaption></figure></p> | <p><figcaption>Figure 1. Recorded OD600 values as noted in the provided Interlab Excel data sheet </figcaption></figure></p> | ||
</br> | </br> | ||
<figure> | <figure> | ||
− | <IMG SRC="https://static.igem.org/mediawiki/2016/0/02/T--Newcastle--Abs600_Graph.png" ALT="some text" WIDTH= | + | <IMG SRC="https://static.igem.org/mediawiki/2016/0/02/T--Newcastle--Abs600_Graph.png" ALT="some text" WIDTH=800 HEIGHT=600> |
<p><figcaption>Figure 2. OD600 values plotted as a graph </figcaption></figure></p> | <p><figcaption>Figure 2. OD600 values plotted as a graph </figcaption></figure></p> | ||
</br> | </br> | ||
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<h3>Fluorescence values</h3> | <h3>Fluorescence values</h3> | ||
<figure> | <figure> | ||
− | <IMG SRC="https://static.igem.org/mediawiki/2016/5/53/T--Newcastle--Fluorescence.png" ALT="some text" WIDTH= | + | <IMG SRC="https://static.igem.org/mediawiki/2016/5/53/T--Newcastle--Fluorescence.png" ALT="some text" WIDTH=800 HEIGHT=600> |
<p><figcaption>Figure 3. Recorded OD600 values as noted in the provided Interlab Excel data sheet </figcaption></figure></p> | <p><figcaption>Figure 3. Recorded OD600 values as noted in the provided Interlab Excel data sheet </figcaption></figure></p> | ||
</br> | </br> | ||
<figure> | <figure> | ||
− | <IMG SRC="https://static.igem.org/mediawiki/2016/8/85/T--Newcastle--Fluorescence_Graph.png" ALT="some text" WIDTH= | + | <IMG SRC="https://static.igem.org/mediawiki/2016/8/85/T--Newcastle--Fluorescence_Graph.png" ALT="some text" WIDTH=800 HEIGHT=600> |
<p><figcaption>Figure 4. OD600 values plotted as a graph </figcaption></figure></p> | <p><figcaption>Figure 4. OD600 values plotted as a graph </figcaption></figure></p> | ||
</br> | </br> | ||
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<h3>Normalised Fluorescence/OD600 values</h3> | <h3>Normalised Fluorescence/OD600 values</h3> | ||
<figure> | <figure> | ||
− | <IMG SRC="https://static.igem.org/mediawiki/2016/c/cf/T--Newcastle--Fluorescence_Abs600.png" ALT="some text" WIDTH= | + | <IMG SRC="https://static.igem.org/mediawiki/2016/c/cf/T--Newcastle--Fluorescence_Abs600.png" ALT="some text" WIDTH=800 HEIGHT=600> |
<p><figcaption>Figure 5. Recorded OD600 values as noted in the provided Interlab Excel data sheet </figcaption></figure></p> | <p><figcaption>Figure 5. Recorded OD600 values as noted in the provided Interlab Excel data sheet </figcaption></figure></p> | ||
</br> | </br> | ||
<figure> | <figure> | ||
− | <IMG SRC="https://static.igem.org/mediawiki/2016/4/43/T--Newcastle--Fluorescence_Abs600_Graph.png" ALT="some text" WIDTH= | + | <IMG SRC="https://static.igem.org/mediawiki/2016/4/43/T--Newcastle--Fluorescence_Abs600_Graph.png" ALT="some text" WIDTH=800 HEIGHT=600> |
<p><figcaption>Figure 6. OD600 values plotted as a graph </figcaption></figure></p> | <p><figcaption>Figure 6. OD600 values plotted as a graph </figcaption></figure></p> | ||
</br></p> | </br></p> | ||
− | <h3> | + | <h1>Flow Cytometry Results</h1> |
+ | <br><br> | ||
+ | |||
+ | <h3>Calibration Particles Peak and Mean Conversion Values</h3> | ||
<figure> | <figure> | ||
− | <IMG SRC="https://static.igem.org/mediawiki/2016/7/7d/T--Newcastle--Flow_Cytometry_1.jpg" ALT="some text" WIDTH= | + | <IMG SRC="https://static.igem.org/mediawiki/2016/7/7d/T--Newcastle--Flow_Cytometry_1.jpg" ALT="some text" WIDTH=800 HEIGHT=200> |
− | <p><figcaption>Figure | + | <p><figcaption>Figure 7. SpheroTech RCP-30-5A Rainbow Calibration Particles were used to obtain mean conversion values.</figcaption></figure></p> |
</br> | </br> | ||
− | <h3> | + | <h3>Geometric Mean MEFL of Negative and Positive Controls and 3 Devices</h3> |
<figure> | <figure> | ||
− | <IMG SRC="https://static.igem.org/mediawiki/2016/3/33/T--Newcastle--Flow_Cytometry_2_.jpg" ALT="some text" WIDTH= | + | <IMG SRC="https://static.igem.org/mediawiki/2016/3/33/T--Newcastle--Flow_Cytometry_2_.jpg" ALT="some text" WIDTH=2100 HEIGHT=150> |
− | <p><figcaption>Figure | + | <p><figcaption>Figure 8. Geometric mean MEFL values were calculated by measuring the number of events and geometric mean values in 5 samples and using mean conversion value from the table above.</figcaption></figure></p> |
</br> | </br> | ||
</html> | </html> |
Latest revision as of 22:22, 19 October 2016