Difference between revisions of "Team:Paris Saclay/Notebook/October/18"
(Created page with "{{Team:Paris_Saclay/notebook_header}} = Monday 17<sup>th</sup> October= ===Visualization=== ====Electro transformation of DH5a cells with K2039002==== ''By Sylvie'' 4 samp...") |
(→Electro transformation of DH5a cells with K2039002) |
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
| − | = | + | = Tuesday 18<sup>th</sup> October= |
===Visualization=== | ===Visualization=== | ||
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''By Sylvie'' | ''By Sylvie'' | ||
| − | 4 samples of DH5a cells were transformed using | + | 4 samples of DH5a cells were transformed using electroporation protocol using: |
| − | + | * 1µL of K2039002 preparation, diluted 10 times | |
| − | + | * 1 µL of K2039002 preparation, no dilution | |
| − | + | * 5 µL of K2039002 preparation, no dilution | |
| + | * 1 control (without DNA) | ||
Transformed cells were plated on LB solid media with Chloramphenicol and Ampicilin. | Transformed cells were plated on LB solid media with Chloramphenicol and Ampicilin. | ||
Latest revision as of 22:58, 19 October 2016
