Difference between revisions of "Team:Paris Saclay/Notebook/October/18"
(→Monday 17th October) |
(→Electro transformation of DH5a cells with K2039002) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 8: | Line 8: | ||
''By Sylvie'' | ''By Sylvie'' | ||
| − | 4 samples of DH5a cells were transformed using | + | 4 samples of DH5a cells were transformed using electroporation protocol using: |
| − | + | * 1µL of K2039002 preparation, diluted 10 times | |
| − | + | * 1 µL of K2039002 preparation, no dilution | |
| − | + | * 5 µL of K2039002 preparation, no dilution | |
| + | * 1 control (without DNA) | ||
Transformed cells were plated on LB solid media with Chloramphenicol and Ampicilin. | Transformed cells were plated on LB solid media with Chloramphenicol and Ampicilin. | ||
Latest revision as of 22:58, 19 October 2016
