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<h4 align="center"> There are many advantages to targeting mRNA. If any mistakes happen, they will not be permanent because the DNA is not being edited. The system will be regulated with doxycycline, and we can double the possible number of edits. Not only will we be able to use APOBEC1 and perform C to U edits, but we will also be able to use the editing enzyme ADAR, which performs A to I edits exclusively in RNA. Inosine (I) is a nucleotide that is common in brain pathways and is read by the ribosome as a G. By combining all of these concepts together, we developed a system to target single nucleotide changes in RNA. You can read more about the specifics of our system in our <a href="https://2016.igem.org/Team:WPI_Worcester/Design"> Design </a>.</h4> | <h4 align="center"> There are many advantages to targeting mRNA. If any mistakes happen, they will not be permanent because the DNA is not being edited. The system will be regulated with doxycycline, and we can double the possible number of edits. Not only will we be able to use APOBEC1 and perform C to U edits, but we will also be able to use the editing enzyme ADAR, which performs A to I edits exclusively in RNA. Inosine (I) is a nucleotide that is common in brain pathways and is read by the ribosome as a G. By combining all of these concepts together, we developed a system to target single nucleotide changes in RNA. You can read more about the specifics of our system in our <a href="https://2016.igem.org/Team:WPI_Worcester/Design"> Design </a>.</h4> | ||
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+ | <h3 align="center"><b>References</b></h3> | ||
+ | <h4>O'Connell, M.R., et al.(2014).Programmable RNA recognition and cleavage by CRISPR/Cas9.<i>Nature</i>.516:263-266.<br><br> | ||
+ | Komor, A.C., et al.(2016).Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.<i> Nature</i>533:420-424.<br><br> | ||
+ | Nelles DA et al. (2016).Programmable RNA Tracking in Live Cells with CRISPR/Cas9.<i>Cell</i>.165:488-496.<h4> | ||
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Latest revision as of 23:53, 19 October 2016