Difference between revisions of "Team:Austin UTexas/Parts"

 
(3 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{Austin_UTexas}}
 
{{Austin_UTexas}}
 
<html>
 
<html>
 +
<head>
 +
<style>
  
−
<div class="column full_size">
+
body {
−
<h1>Austin_UTexas</h1>
+
    background-image: url(https://static.igem.org/mediawiki/2016/a/ad/T--Austin_UTexas--partsbg.jpg);
 +
    background-repeat: no-repeat;
 +
    background-position: 0px 0px;
 +
    background-attachment:fixed;
 +
    background-size: 100%;
 +
}
 +
 
 +
</style>
 +
</head>
 +
<body>
 +
 
 +
<div class="column full_size_outer">
 +
<div class="column full_size_inner">
 
<h2>Registry Parts</h2>
 
<h2>Registry Parts</h2>
 
<br>
 
<br>
−
 
+
<p> We submitted three parts to the registry.  These parts are described below.  The link to each page is shown in bold text.</p>
  
 
<div class="column half_size">
 
<div class="column half_size">
Line 12: Line 26:
 
<br>
 
<br>
 
<h4>CpxA-CpxR</h4>
 
<h4>CpxA-CpxR</h4>
−
<p>CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. The construct can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097000">K2097000</a>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results">here</a>.</p>
+
<p>CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. <b>The BioBrick can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097000">K2097000</a></b>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results#section6">here</a>.</p>
 
</div>
 
</div>
 +
 +
 
<div class="column half_size" >
 
<div class="column half_size" >
 
<h3>Composite Parts</h3>
 
<h3>Composite Parts</h3>
 
<br>
 
<br>
 
<h4>Blue Chromoprotein</h4>
 
<h4>Blue Chromoprotein</h4>
−
<p>This is a composite part made up of <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> (blue chromoprotein) and <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002  </a> (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> is made up of a promoter, <a href="http://partsregistry.org/Part:BBa_J23104"> BBa_J23104</a>, and an RBS, <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_B0034</a>. The construct can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097001">K2097001</a>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results">here</a>.</p>
+
<p>This is a composite part made up of <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> (blue chromoprotein) and <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002  </a> (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_K608002 </a> is made up of a promoter, <a href="http://partsregistry.org/Part:BBa_J23104"> BBa_J23104</a>, and an RBS, <a href="http://partsregistry.org/Part:BBa_K608002"> BBa_B0034</a>. <b>The BioBrick can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097001">K2097001</a></b>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results">here</a>.</p>
 
<br>
 
<br>
 
<h4>Yellow-Green Chromoprotein</h4>
 
<h4>Yellow-Green Chromoprotein</h4>
−
<p>This is a composite part made up of <a href="http://parts.igem.org/Part:BBa_K1033916">BBa_K1033916</a> (amajLime, a yellow-green chromoprotein) and <a href="http://parts.igem.org/Part:BBa_K608006"> BBa_K608006</a> (a medium promoter and RBS). It is made up of a medium promoter, <a href="http://parts.igem.org/Part:BBa_J23110"> BBa_J23110</a>, and a medium RBS, <a href="http://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>. The construct can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097002">K2097002</a>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results">here</a></p>
+
<p>This is a composite part made up of <a href="http://parts.igem.org/Part:BBa_K1033916">BBa_K1033916</a> (amajLime, a yellow-green chromoprotein) and <a href="http://parts.igem.org/Part:BBa_K608006"> BBa_K608006</a> (a medium promoter and RBS). It is made up of a medium promoter, <a href="http://parts.igem.org/Part:BBa_J23110"> BBa_J23110</a>, and a medium RBS, <a href="http://parts.igem.org/Part:BBa_B0032"> BBa_B0032</a>. <b>The BioBrick can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097002">K2097002</a></b>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results">here</a></p>
 
</div>
 
</div>
 
</html>
 
</html>
Line 28: Line 44:
 
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 +
  
 
<div class="column half_size">
 
<div class="column half_size">
Line 35: Line 52:
 
</div>
 
</div>
 
</div>
 
</div>
−
 
−
 
  
  
Line 45: Line 60:
 
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
</div>
 
</div>
−
 
−
 
−
 
  
  

Latest revision as of 00:03, 20 October 2016

Registry Parts


We submitted three parts to the registry. These parts are described below. The link to each page is shown in bold text.

pH Sensors


CpxA-CpxR

CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in Shigella sonnei, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. The BioBrick can be found on the iGEM registry as: K2097000, as well as on our results page here.

Composite Parts


Blue Chromoprotein

This is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B0034. The BioBrick can be found on the iGEM registry as: K2097001, as well as on our results page here.


Yellow-Green Chromoprotein

This is a composite part made up of BBa_K1033916 (amajLime, a yellow-green chromoprotein) and BBa_K608006 (a medium promoter and RBS). It is made up of a medium promoter, BBa_J23110, and a medium RBS, BBa_B0032. The BioBrick can be found on the iGEM registry as: K2097002, as well as on our results page here