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| {{Alverno_CA}} | | {{Alverno_CA}} |
| <html> | | <html> |
| + | <script src="http://wayou.github.io/SlipHover/js/jquery.sliphover.min.js"> |
| + | </script> |
| + | <style type="text/css"> |
| + | #container{ |
| + | width: 750px; |
| + | margin: 0 auto; |
| + | text-align:center; |
| + | } |
| + | #container img{ |
| + | margin:10px 10px 0px 0px; |
| + | float: left; |
| + | } |
| + | #container div{ |
| + | width: 650px; |
| + | overflow: hidden; |
| + | } |
| + | #container ul li{ |
| + | list-style: none; |
| + | } |
| + | .sliphover-container{ |
| + | z-index:99; |
| + | } |
| + | #menuContainer{ |
| + | top:0px; |
| + | margin-top:0px; |
| + | position:absolute; |
| + | } |
| + | #top_nav_bulge{ |
| + | top:110px; |
| + | } |
| + | </style> |
| + | |
| + | |
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| <head> | | <head> |
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| <br> | | <br> |
| <center><img src="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png" alt="Alverno iGEM Logo" style="width:300px;"></center> | | <center><img src="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png" alt="Alverno iGEM Logo" style="width:300px;"></center> |
− | <h1><center>Proof</center></h1>
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− | <p>Working Model of Software - Python Program (Code) for Analyzing Plate Reader Data
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− | *The plate reader we use is the Perkin Elmer Victor X3 2030 Multilabel Reader.
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− | *The results will show up as graphs.
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| | | |
− | import matplotlib.pyplot as plt
| + | <center><h2>Proof of Concept</h2></center> |
− | import pandas as pd
| + | <div class="demo" id="container"> |
− | import seaborn as sns
| + | |
− | #The name of the file you plan to use goes in the ‘’ for filename the line below.
| + | |
− | filename = ''
| + | |
− | df = pd.read_csv(filename)
| + | |
− | df = df.dropna()
| + | |
− | print df.head()
| + | |
| | | |
− | # data = df[df['Well']=='A03']
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− | # data = df[df['Repeat'] > 115]
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− | #The two lines above may be used if you want to test some wells that only satisfy specific conditions, for example Well A01, and all the repeat above 115.
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− | data = df[df['Well'].isin(['H06','H07','H08','H09'])]
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− | #The line above is used to test a collection of wells by their well name. You can add the names of the wells into [].
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| | | |
− | #The following are the different wells on the plate we used, you may use something different
| + | <h3>To find out more about our Proof of Concept see our |
− | #'A01','A02','A03','A04','A05','A06','A07','A08','A09','A10','A11','A12'
| + | <a href="https://2016.igem.org/Team:Alverno_CA/Results"> Results page.</a><h3> |
− | #'B01','B02','B03','B04','B05','B06','B07','B08','B09','B10','B11','B12'
| + | </body> |
− | #'C01','C02','C03','C04','C05','C06','C07','C08','C09','C10','C11','C12'
| + | |
− | #'D01','D02','D03','D04','D05','D06','D07','D08','D09','D10','D11','D12'
| + | |
− | #'E01','E02','E03','E04','E05','E06','E07','E08','E09','E10','E11','E12'
| + | |
− | #'F01','F02','F03','F04','F05','F06','F07','F08','F09','F10','F11','F12'
| + | |
− | #'G01','G02','G03','G04','G05','G06','G07','G08','G09','G10','G11','G12'
| + | |
− | #'H01','H02','H03','H04','H05','H06','H07','H08','H09','H10','H11','H12'
| + | |
| | | |
| | | |
− | sns.tsplot(data=data, time="Time RFP (seconds)",
| + | <script> |
− | value="Normalized RFP", condition="Well",
| + | $("#container").sliphover(); |
− | err_style="unit_traces", unit="Plate")
| + | $(".sliphover-container").css('z-index','99'); |
− | plt.title("RFP Fluorescence")
| + | $("#menuContainer").css('z-index','99'); |
− | plt.ylabel("RFP(AFU)")
| + | </script> |
| | | |
− | plt.show()
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− |
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− | sns.tsplot(data=data, time="Time GFP (seconds)",
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− | value="Normalized GFP", condition="Well",
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− | err_style="unit_traces", unit="Well")
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− | plt.title("GFP Fluorescence")
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− | plt.ylabel("GFP(AFU)")
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− |
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− | plt.show()
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− |
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− | sns.tsplot(data=data, time="Time Abs (seconds)",
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− | value="Absorbance @ 600 (A)", condition="Well",
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− | err_style="unit_traces", unit="Well")
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− | plt.title("Absorbance")
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− | plt.ylabel("Absorbance(AAU)")
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− |
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− | plt.show()
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− |
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− | BioBrick Assembly: Plasmid with pSB1C3 Backbone
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− | ??
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− |
| |
− | </p>
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− | </body>
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| </html> | | </html> |