Difference between revisions of "Team:Alverno CA/Proof"

 
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{{Alverno_CA}}
 
{{Alverno_CA}}
 
<html>
 
<html>
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  <script src="http://wayou.github.io/SlipHover/js/jquery.sliphover.min.js">
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    </script>
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    <style type="text/css">
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    #container{
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    width: 750px;
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    margin: 0 auto;
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                text-align:center;
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                z-index:99;
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<head>
 
<head>
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<br>
 
<br>
 
<center><img src="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png" alt="Alverno iGEM Logo" style="width:300px;"></center>
 
<center><img src="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png" alt="Alverno iGEM Logo" style="width:300px;"></center>
<h1><center>Proof</center></h1>
 
<p>Working Model of Software - Python Program (Code) for Analyzing Plate Reader Data
 
*The plate reader we use is the Perkin Elmer Victor X3 2030 Multilabel Reader.
 
*The results will show up as graphs.
 
  
import matplotlib.pyplot as plt
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<center><h2>Proof of Concept</h2></center>
import pandas as pd
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<div class="demo" id="container">
import seaborn as sns
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#The name of the file you plan to use goes in the ‘’ for filename the line below.
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filename = ''
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df = pd.read_csv(filename)
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df = df.dropna()
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print df.head()
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# data = df[df['Well']=='A03']
 
# data = df[df['Repeat'] > 115]
 
#The two lines above may be used if you want to test some wells that only satisfy specific conditions, for example Well A01, and all the repeat above 115.
 
data = df[df['Well'].isin(['H06','H07','H08','H09'])]
 
#The line above is used to test a collection of wells by their well name. You can add the names of the wells into [].
 
  
#The following are the different wells on the plate we used, you may use something different
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<h3>To find out more about our Proof of Concept see our
#'A01','A02','A03','A04','A05','A06','A07','A08','A09','A10','A11','A12'
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<a href="https://2016.igem.org/Team:Alverno_CA/Results"> Results page.</a><h3>
#'B01','B02','B03','B04','B05','B06','B07','B08','B09','B10','B11','B12'
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</body>
#'C01','C02','C03','C04','C05','C06','C07','C08','C09','C10','C11','C12'
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#'D01','D02','D03','D04','D05','D06','D07','D08','D09','D10','D11','D12'
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#'E01','E02','E03','E04','E05','E06','E07','E08','E09','E10','E11','E12'
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#'F01','F02','F03','F04','F05','F06','F07','F08','F09','F10','F11','F12'
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#'G01','G02','G03','G04','G05','G06','G07','G08','G09','G10','G11','G12'
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#'H01','H02','H03','H04','H05','H06','H07','H08','H09','H10','H11','H12'
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sns.tsplot(data=data, time="Time RFP (seconds)",
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<script>
          value="Normalized RFP", condition="Well",
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    $("#container").sliphover();
          err_style="unit_traces", unit="Plate")
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        $(".sliphover-container").css('z-index','99');
plt.title("RFP Fluorescence")
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        $("#menuContainer").css('z-index','99');
plt.ylabel("RFP(AFU)")
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        </script>
  
plt.show()
 
 
sns.tsplot(data=data, time="Time GFP (seconds)",
 
          value="Normalized GFP", condition="Well",
 
          err_style="unit_traces", unit="Well")
 
plt.title("GFP Fluorescence")
 
plt.ylabel("GFP(AFU)")
 
 
plt.show()
 
 
sns.tsplot(data=data, time="Time Abs (seconds)",
 
          value="Absorbance @ 600 (A)", condition="Well",
 
          err_style="unit_traces", unit="Well")
 
plt.title("Absorbance")
 
plt.ylabel("Absorbance(AAU)")
 
 
plt.show()
 
 
BioBrick Assembly: Plasmid with pSB1C3 Backbone
 
??
 
 
</p>
 
</body>
 
 
</html>
 
</html>

Latest revision as of 00:24, 20 October 2016

Alverno iGEM 2016

Alverno iGEM Logo

Proof of Concept

To find out more about our Proof of Concept see our Results page.