Difference between revisions of "Team:Technion Israel/Protocols"

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<style>/* inline page CSS */
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body {
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}
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/* ==========Background and effects ========== */
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/*Make sure the div is unuiqe to each page*/
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.desk_wrapper {
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position: relative;
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background: white; /* For browsers that do not support gradients */
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background: -webkit-linear-gradient(white, #ecf7fb, white); /* For Safari 5.1 to 6.0 */
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background: -o-linear-gradient(white, #ecf7fb, white); /* For Opera 11.1 to 12.0 */
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background: -moz-linear-gradient(white, #ecf7fb, white); /* For Firefox 3.6 to 15 */
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background: linear-gradient(white, #ecf7fb, white); /* Standard syntax */
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/*background-image:url('addres');
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background-size:cover;
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filter: blur(5px) grayscale(80%) opacity(10%);*/
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}
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/* ========== END: and effects ========== */
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/*
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Object in external CSS sheet:
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nav-tabs, cont_tabs:
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Open diffrent tabs, we uses imgs.
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back-to-top:
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go back to top. Apears only when going down the page.
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cont_box:
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The containers (box) which hold the texts and imgs in the page.
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img_cont:
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Every in-content-page img needs to have this class of img.
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no-title-col:
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Every in-content-page img needs to have this class of col.
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*/
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.referances {
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  font-size: 16px;
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outline: 1px solid black;
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padding: 20px;
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}
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/*canceling wiki bug (inlarge imgs stuck the page)*/
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.modal-backdrop {
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position: relative;
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}
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.ocenter .img-responsive {
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margin: 0 auto;
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}
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.vcenter {
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display: inline-block;
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vertical-align: middle;
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float: none;
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}
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.table{
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font-family: "Candara", "Calibri", "Segoe", "Segoe UI", "Optima", Arial, sans-serif;
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    font-size: 18px;
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font-style: normal;
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font-variant: normal;
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font-weight: 400;
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line-height: 27px;
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cursor: pointer;
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transition: all .5s ease;
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margin: 0 auto;
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/* border: 2px solid #bbb;
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box-shadow: 5px 5px 3px grey;
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padding: 15px; */
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.cir_tabs:hover{
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-webkit-filter: grayscale(0);
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transform: scale(1.2,1.2);
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margin: 0 auto;
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/*border: 2px solid #000;
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box-shadow: 5px 5px 3px grey;*/
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}
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</style>
  
 
<style>/* inline page CSS */
 
<style>/* inline page CSS */
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<a href="https://static.igem.org/mediawiki/2016/f/f9/T--Technion_Israel--0.1_M_potassium_phosphate_%28KPi%29.pdf" target="_blank">KPi Preparation</a>
 
<a href="https://static.igem.org/mediawiki/2016/f/f9/T--Technion_Israel--0.1_M_potassium_phosphate_%28KPi%29.pdf" target="_blank">KPi Preparation</a>
 
<br><br>
 
<br><br>
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<a href="https://static.igem.org/mediawiki/2016/7/77/T--Technion_Israel--PDMSprotocol.pdf" target="_blank">Fabrication of a PDMS chip</a>
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<br><br>
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<a href="https://static.igem.org/mediawiki/2016/4/46/Gel_electrophoresis_protocol.pdf" target="_blank">Gel electrophoresis </a>
 
</p>
 
</p>
 
</div>
 
</div>
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<div class="col-md-6 col-sm-12">
 
<div class="col-md-6 col-sm-12">
 
<p class="text-justify">
 
<p class="text-justify">
−
<a href="https://static.igem.org/mediawiki/2016/4/46/Gel_electrophoresis_protocol.pdf" target="_blank">Gel electrophoresis </a>
 
−
<br><br>
 
 
<a href="https://static.igem.org/mediawiki/2016/1/14/T--Technion_Israel--Gel_Extraction_%28to_miniprep%29.pdf" target="_blank">Gel Extraction</a>
 
<a href="https://static.igem.org/mediawiki/2016/1/14/T--Technion_Israel--Gel_Extraction_%28to_miniprep%29.pdf" target="_blank">Gel Extraction</a>
 
<br><br>
 
<br><br>
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<a href="https://static.igem.org/mediawiki/2016/d/dc/T--Technion_Israel--Glycerol_stock.pdf" target="_blank">Glycerol Stock</a>
 
<a href="https://static.igem.org/mediawiki/2016/d/dc/T--Technion_Israel--Glycerol_stock.pdf" target="_blank">Glycerol Stock</a>
 
<br><br>
 
<br><br>
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<a href="https://static.igem.org/mediawiki/2016/2/22/T--Technion_Israel--mediumprotocol.pdf" target="_blank">Growing medium assays</a><br><br>
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<br><br>
 
<a href="https://static.igem.org/mediawiki/2016/e/ef/T--Technion_Israel--PCR_protocol.pdf" target="_blank">PCR</a>
 
<a href="https://static.igem.org/mediawiki/2016/e/ef/T--Technion_Israel--PCR_protocol.pdf" target="_blank">PCR</a>
 
<br><br>
 
<br><br>
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<br><br>
 
<br><br>
 
<a href="https://static.igem.org/mediawiki/2016/f/f3/T--Technion_Israel--Chemotaxis_assays.pdf" target="_blank">Swarming assay</a>
 
<a href="https://static.igem.org/mediawiki/2016/f/f3/T--Technion_Israel--Chemotaxis_assays.pdf" target="_blank">Swarming assay</a>
−
<br><br>
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</p>
−
<a href="https://static.igem.org/mediawiki/2016/7/77/T--Technion_Israel--PDMSprotocol.pdf" target="_blank">Fabrication of a PDMS chip</a>
+
−
<br><br>
+
−
<a href="https://static.igem.org/mediawiki/2016/2/22/T--Technion_Israel--mediumprotocol.pdf" target="_blank">Growing medium assays</a><br><br>
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−
 
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</p>
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</div>
 
</div>

Revision as of 00:38, 20 October 2016

S.tar, by iGEM Technion 2016

S.tar, by iGEM Technion 2016



Agarose Gel Preparation

Chemical Transformation

Chemotaxis on chip test

Colony PCR

DpnI

In plug assay

Intein Incubation

KPi Preparation

Fabrication of a PDMS chip

Gel electrophoresis

Gel Extraction

Gibson Assembly

Glycerol Stock

Growing medium assays



PCR

Restriction and Ligation

SOB frozen competent cells

Swarming assay




S.tar, by iGEM Technion 2016