Difference between revisions of "Team:Virginia/Parts"

 
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We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ)
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cleavage enzyme part, and a composite part consisting of a promoter, the CBZ
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cleavage enzyme, and a terminator.</span></p>
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<div style="float:right"><img class="mySlides" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png" style="width:750px">
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<p><span class="p"> <b>Overview</b></p>
 
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<p>We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
 
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This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence.
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<br><br><span class="ptitle">Bba_K1879001</span><br><br>
 
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This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
 
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<span class="ptitle">BBa_K1879000</span><br><br>
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<p><span class="p">
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This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
 +
cleavage enzyme necessary to remove the CBZ protecting group from the
 +
N-terminus of amino acids. The coding sequence was derived from Nanduri
 +
et al<span class="references"><sup>2</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
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</span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p>
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<br><br><br><br>       
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<span class="ptitle">BBa_K1879001</span><br><br>
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<p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage
 +
enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our
 +
biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce
 +
wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively
 +
cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
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</span></p>
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Latest revision as of 00:38, 20 October 2016

←Experiments Summary →

We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.



❮ ❯
BBa_K1879000

This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al2Reference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. , and we included an RBS sequence upstream of the coding sequence.





BBa_K1879001

This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.