Difference between revisions of "Team:Virginia/Parts"

Latest revision as of 00:38, 20 October 2016

We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.

❮ ❯

BBa_K1879000

This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al²Reference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. , and we included an RBS sequence upstream of the coding sequence.

BBa_K1879001

This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.

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-		<!---------------------------Page title area-------------------------------------------->
-		<!--<div class="pagetitle">
-			<h1 style="color:#66b3ff">Insert title of page here!</h1>
-		</div>-->
 		<!---------------------------Main content section of page------------------------------->
 		<div class="content">
-		<p><span class="p"> <b>Overview</b></p>
+<p><span class="p">
-<p>We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
+We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ)
-</p>
+cleavage enzyme part, and a composite part consisting of a promoter, the CBZ
+cleavage enzyme, and a terminator.</span></p>
+	<br>
+<br>
+<div style="float:right"><img class="mySlides" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png" style="width:750px">
+  <img class="mySlides" src="https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png" style="width:750px">
+<a class="w3-btn-floating" style="position:absolute;top:700px;left:450px;font-size:24px;color:#235c81;text-decoration:none;cursor:pointer" onclick="plusDivs(-1)">❮</a>
+  <a class="w3-btn-floating" style="position:absolute;top:700px;right:145px;font-size:24px;color:#235c81;text-decoration:none;cursor:pointer" onclick="plusDivs(1)">❯</a>
+<script>
+var slideIndex = 1;
+showDivs(slideIndex);
+function plusDivs(n) {
+  showDivs(slideIndex += n);
+}
+function showDivs(n) {
+  var i;
+  var x = document.getElementsByClassName("mySlides");
+  if (n > x.length) {slideIndex = 1}
+  if (n < 1) {slideIndex = x.length}
+  for (i = 0; i < x.length; i++) {
+     x[i].style.display = "none";
+  }
+  x[slideIndex-1].style.display = "block";
+}
+</script></div>
+<span class="ptitle">BBa_K1879000</span><br><br>
+<p><span class="p">
+This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
+cleavage enzyme necessary to remove the CBZ protecting group from the
+N-terminus of amino acids. The coding sequence was derived from Nanduri
+et al<span class="references"><sup>2</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
+</span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p>
+<br><br><br><br>
+<span class="ptitle">BBa_K1879001</span><br><br>
+<p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage
+ enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our
+ biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce
+wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively
+cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
+</span></p>
-		<span class="capright"><img style="height:310px; width: 310px" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png"/>
-		<br><i></i></span>
-<span class="ptitle">Bba_K1879000</span><br><br>
-This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence.
-		<br><br><span class="ptitle">Bba_K1879001</span><br><br>
-This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
-			</span></p>
 		</div>
 	</div>