Difference between revisions of "Team:Virginia/Parts"
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cleavage enzyme part, and a composite part consisting of a promoter, the CBZ | cleavage enzyme part, and a composite part consisting of a promoter, the CBZ | ||
cleavage enzyme, and a terminator.</span></p> | cleavage enzyme, and a terminator.</span></p> | ||
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| + | <span class="ptitle">BBa_K1879000</span><br><br> | ||
| + | <p><span class="p"> | ||
| + | This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) | ||
| + | cleavage enzyme necessary to remove the CBZ protecting group from the | ||
| + | N-terminus of amino acids. The coding sequence was derived from Nanduri | ||
| + | et al<span class="references"><sup>2</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. | ||
| + | </span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p> | ||
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| + | <br><br><br><br> | ||
| + | <span class="ptitle">BBa_K1879001</span><br><br> | ||
| + | <p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage | ||
| + | enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our | ||
| + | biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce | ||
| + | wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively | ||
| + | cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions. | ||
| + | </span></p> | ||
Latest revision as of 00:38, 20 October 2016
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
BBa_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
cleavage enzyme necessary to remove the CBZ protecting group from the
N-terminus of amino acids. The coding sequence was derived from Nanduri
et al2Reference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
, and we included an RBS sequence upstream of the coding sequence.
BBa_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
