Difference between revisions of "Team:Virginia/Parts"

 
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We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ)
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cleavage enzyme part, and a composite part consisting of a promoter, the CBZ
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cleavage enzyme, and a terminator.</span></p>
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<div style="float:right"><img class="mySlides" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png" style="width:750px">
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We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ)
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<span class="ptitle">BBa_K1879000</span><br><br>
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cleavage enzyme part, and a composite part consisting of a promoter, the CBZ
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cleavage enzyme, and a terminator.</span></p>
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<span class="ptitle">Bba_K1879000</span><br><br>
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<p><span class="p">
 
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This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) <br>
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This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)  
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cleavage enzyme necessary to remove the CBZ protecting group from the <br>
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cleavage enzyme necessary to remove the CBZ protecting group from the  
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N-terminus of amino acids. The coding sequence was derived from Nanduri <br>
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N-terminus of amino acids. The coding sequence was derived from Nanduri  
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et al<span class="references"><sup>ref</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
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et al<span class="references"><sup>2</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
 
</span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p>
 
</span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p>
 
 
 
<br><br><br><br>         
 
<br><br><br><br>         
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<span class="ptitle">Bba_K1879001</span><br><br>  
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<span class="ptitle">BBa_K1879001</span><br><br>  
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<p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage<br>
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<p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage
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  enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our <br>
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  enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our  
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  biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce<br>
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  biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce  
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wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively <br>
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wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively  
 
cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
 
cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
 
</span></p>
 
</span></p>

Latest revision as of 00:38, 20 October 2016

←Experiments Summary →

We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.



❮ ❯
BBa_K1879000

This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al2Reference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. , and we included an RBS sequence upstream of the coding sequence.





BBa_K1879001

This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.