Difference between revisions of "Team:Virginia/Parts"

 
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--->
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<!-----------------------------------Space reserved for menu------------------------------------>
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<div class="footerspace">
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<a href="https://2016.igem.org/Team:Virginia/Experiments" class="footerleft">&larr;Experiments</a>
 +
<a href="https://2016.igem.org/Team:Virginia/Summary" class="footerright">Summary &rarr;</a>
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<p><span class="p">
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We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ)
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cleavage enzyme part, and a composite part consisting of a promoter, the CBZ
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cleavage enzyme, and a terminator.</span></p>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<div style="float:right"><img class="mySlides" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png" style="width:750px">
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<h5>Note</h5>
 
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
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<span class="ptitle">BBa_K1879000</span><br><br>
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<p><span class="p">
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This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
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cleavage enzyme necessary to remove the CBZ protecting group from the
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N-terminus of amino acids. The coding sequence was derived from Nanduri
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et al<span class="references"><sup>2</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
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</span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p>
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<br><br><br><br>       
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<span class="ptitle">BBa_K1879001</span><br><br>
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<p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage
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enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our
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biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce
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wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively
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cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
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</span></p>
  
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<h5>Adding parts to the registry</h5>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
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<h5>What information do I need to start putting my parts on the Registry?</h5>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<groupparts>iGEM2016 Example</groupparts>
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Latest revision as of 00:38, 20 October 2016

←Experiments Summary →

We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.



❮ ❯
BBa_K1879000

This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al2Reference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. , and we included an RBS sequence upstream of the coding sequence.





BBa_K1879001

This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.