Difference between revisions of "Team:Virginia/Parts"
| (8 intermediate revisions by the same user not shown) | |||
| Line 26: | Line 26: | ||
<div class="content"> | <div class="content"> | ||
| + | <p><span class="p"> | ||
| + | We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) | ||
| + | cleavage enzyme part, and a composite part consisting of a promoter, the CBZ | ||
| + | cleavage enzyme, and a terminator.</span></p> | ||
| + | <br> | ||
| + | <br> | ||
| + | <div style="float:right"><img class="mySlides" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png" style="width:750px"> | ||
| + | <img class="mySlides" src="https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png" style="width:750px"> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| + | <a class="w3-btn-floating" style="position:absolute;top:700px;left:450px;font-size:24px;color:#235c81;text-decoration:none;cursor:pointer" onclick="plusDivs(-1)">❮</a> | ||
| + | <a class="w3-btn-floating" style="position:absolute;top:700px;right:145px;font-size:24px;color:#235c81;text-decoration:none;cursor:pointer" onclick="plusDivs(1)">❯</a> | ||
<script> | <script> | ||
var slideIndex = 1; | var slideIndex = 1; | ||
| Line 61: | Line 64: | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<span class="ptitle">BBa_K1879000</span><br><br> | <span class="ptitle">BBa_K1879000</span><br><br> | ||
<p><span class="p"> | <p><span class="p"> | ||
| Line 71: | Line 70: | ||
cleavage enzyme necessary to remove the CBZ protecting group from the | cleavage enzyme necessary to remove the CBZ protecting group from the | ||
N-terminus of amino acids. The coding sequence was derived from Nanduri | N-terminus of amino acids. The coding sequence was derived from Nanduri | ||
| − | et al<span class="references"><sup> | + | et al<span class="references"><sup>2</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. |
</span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p> | </span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p> | ||
Latest revision as of 00:38, 20 October 2016
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
BBa_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
cleavage enzyme necessary to remove the CBZ protecting group from the
N-terminus of amino acids. The coding sequence was derived from Nanduri
et al2Reference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
, and we included an RBS sequence upstream of the coding sequence.
BBa_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
