Difference between revisions of "Team:Peking/Proof"

 
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                             <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Achievements</a>
 
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                                     <li><a href="https://2016.igem.org/Team:Peking/Demonstrate" >Results</a></li>
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                                     <li><a href="https://2016.igem.org/Team:Peking/Basic_Part" >Parts</a></li>
 
                                     <li><a href="https://2016.igem.org/Team:Peking/Collaborations" >Collaborations</a></li>
 
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                                 <p style="text-align:center;"><a href="https://2016.igem.org/Team:Peking/Model/MassDistribution"><img style="width:  %;" src="https://static.igem.org/mediawiki/2016/d/da/T--Peking--images_modelgelpoint.png " alt="" /></p></a>
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                                 <p style="text-align:center;"><a href="https://2016.igem.org/Team:Peking/Crosslinking"><img style="width:  %;" src="https://static.igem.org/mediawiki/2016/b/b3/T--Peking--image_H1.png" alt="" /></p></a>
 
                                 <p style="font-family:raleway-bold, sans-serif !important;font-size:24px !important; text-align:center; margin-top:20px;">Crosslinking</p>
 
                                 <p style="font-family:raleway-bold, sans-serif !important;font-size:24px !important; text-align:center; margin-top:20px;">Crosslinking</p>
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                                 <p style="font-family:raleway, sans-serif !important; font-weight:100;font-size:18px !important; text-align:justify;"></p>
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                                 <p style="font-family:raleway, sans-serif !important; font-weight:100;font-size:18px !important; text-align:justify;">By taking advantage of the covalent crosslinking of SpyTag and SpyCatcher, a polymer network was designed to possess special properties, such as strong mechanical strength, large contact area and stable structure.</p>
 
                                  
 
                                  
 
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                                 <p style="text-align:center;"><a href="https://2016.igem.org/Team:Peking/Model/MassDistribution"><img style="width:  %;" src="https://static.igem.org/mediawiki/2016/0/0b/T--Peking--image_modelmass.png" /></p></a>
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                                 <p style="text-align:center;"><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption"><img style="width:  %;" src="https://static.igem.org/mediawiki/2016/b/b1/T--Peking--image_pr2.png" /></p></a>
 
                                 <p style="font-family:raleway-bold, sans-serif !important;font-size:24px !important; text-align:center; margin-top:20px;">Uranyl Adsorption</p>
 
                                 <p style="font-family:raleway-bold, sans-serif !important;font-size:24px !important; text-align:center; margin-top:20px;">Uranyl Adsorption</p>
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                                 <p style="font-family:raleway, sans-serif !important; font-weight:100;font-size:18px !important; text-align:justify;">The Peking IGEM Team has studied and expanded Flory J Paul's method to predict the molecular weight distribution of polymer. They compared their reaper’s experimental molecular weight distribution data with the theoretical one and find it is necessary to improve the polymer network's orthogonality with its functional protein. To optimize the reaper's design, they have brought up some compare standards </p>
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                                 <p style="font-family:raleway, sans-serif !important; font-weight:100;font-size:18px !important; text-align:justify;">Super Uranyl-binding Protein (SUP) is stable and highly selective for uranyl which may cause negative impact on the environment. Triple SpyTag-SUP (3A-SUP) was constructed to adsorb uranyl, and the cross-linked product of 3A-SUP and 3B showed an even better adsorption capacity.</p>
 
                                  
 
                                  
 
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                                 <p style="text-align:center;"><a href="https://2016.igem.org/Team:Peking/Software"><img style="width:  %;" src="https://static.igem.org/mediawiki/2016/c/cc/T--Peking--image_softwarelogo.png" alt=""/></p></a>
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                                 <p style="text-align:center;"><a href="https://2016.igem.org/Team:Peking/Clearance"><img style="width:  %;" src="https://static.igem.org/mediawiki/2016/9/95/T--Peking--image_H3.png" alt=""/></p></a>
 
                                 <p style="font-family:raleway-bold, sans-serif !important;font-size:24px !important; text-align:center; margin-top:20px;">Clearance</p>
 
                                 <p style="font-family:raleway-bold, sans-serif !important;font-size:24px !important; text-align:center; margin-top:20px;">Clearance</p>
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                                 <p style="font-family:raleway, sans-serif !important; font-weight:100;font-size:18px !important; text-align:justify;">SoP is originally built to help our team members work out molecular weight distribution results more efficiently, at least more efficient than excel. Now for the wish of sharing, the software was published on webpage so that everyone can try our method or even compare it with experiments. Note that this application exclusively uses the formula described in our "Model-Mass Distribution" part.</p>
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                                 <p style="font-family:raleway, sans-serif !important; font-weight:100;font-size:18px !important; text-align:justify;">The monomeric streptavidin constructed in the polymer network could realize the clearance of Spy Crosslinking Network in the environment by interacting with biotinylated magnetic beads.</p>
 
                                  
 
                                  
 
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                                 <p style="text-align:center;"><a href="https://2016.igem.org/Team:Peking/Secretion"><img style="width:  %;" src="https://static.igem.org/mediawiki/2016/1/1f/T--Peking--image_H2.png" alt=""/></p></a>
 
                                 <p style="font-family:raleway-bold, sans-serif !important;font-size:24px !important; text-align:center; margin-top:20px;">Secretion</p>
 
                                 <p style="font-family:raleway-bold, sans-serif !important;font-size:24px !important; text-align:center; margin-top:20px;">Secretion</p>
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                                 <p style="font-family:raleway, sans-serif !important; font-weight:100;font-size:18px !important; text-align:justify;">SoP is originally built to help our team members work out molecular weight distribution results more efficiently, at least more efficient than excel. Now for the wish of sharing, the software was published on webpage so that everyone can try our method or even compare it with experiments. Note that this application exclusively uses the formula described in our "Model-Mass Distribution" part.</p>
+
                                 <p style="font-family:raleway, sans-serif !important; font-weight:100;font-size:18px !important; text-align:justify;">To make the polymer network reproductive and economical, a signal peptide library was constructed. We carried out a series of experiments to find an optimal secretion strategy.</p>
 
                                  
 
                                  
 
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Latest revision as of 00:40, 20 October 2016

Proof

Proof of Concept

Crosslinking

By taking advantage of the covalent crosslinking of SpyTag and SpyCatcher, a polymer network was designed to possess special properties, such as strong mechanical strength, large contact area and stable structure.

Uranyl Adsorption

Super Uranyl-binding Protein (SUP) is stable and highly selective for uranyl which may cause negative impact on the environment. Triple SpyTag-SUP (3A-SUP) was constructed to adsorb uranyl, and the cross-linked product of 3A-SUP and 3B showed an even better adsorption capacity.

Clearance

The monomeric streptavidin constructed in the polymer network could realize the clearance of Spy Crosslinking Network in the environment by interacting with biotinylated magnetic beads.

Secretion

To make the polymer network reproductive and economical, a signal peptide library was constructed. We carried out a series of experiments to find an optimal secretion strategy.


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