Difference between revisions of "Team:Technion Israel/Protocols"

 
(One intermediate revision by the same user not shown)
Line 8: Line 8:
 
     <meta name="navbar" content="width=device-width, initial-scale=1">
 
     <meta name="navbar" content="width=device-width, initial-scale=1">
  
−
 
−
 
−
<style>/* inline page CSS */
 
−
 
−
body {
 
−
}
 
−
 
−
/* ==========Background and effects ========== */
 
−
/*Make sure the div is unuiqe to each page*/
 
−
.desk_wrapper {
 
−
position: relative;
 
−
background: white; /* For browsers that do not support gradients */
 
−
background: -webkit-linear-gradient(white, #ecf7fb, white); /* For Safari 5.1 to 6.0 */
 
−
background: -o-linear-gradient(white, #ecf7fb, white); /* For Opera 11.1 to 12.0 */
 
−
background: -moz-linear-gradient(white, #ecf7fb, white); /* For Firefox 3.6 to 15 */
 
−
background: linear-gradient(white, #ecf7fb, white); /* Standard syntax */
 
−
/*background-image:url('addres');
 
−
background-size:cover;
 
−
filter: blur(5px) grayscale(80%) opacity(10%);*/
 
−
}
 
−
/* ========== END: and effects ========== */
 
−
 
−
 
−
/*
 
−
Object in external CSS sheet:
 
−
 
−
nav-tabs, cont_tabs:
 
−
Open diffrent tabs, we uses imgs.
 
−
 
−
back-to-top:
 
−
go back to top. Apears only when going down the page.
 
−
 
−
cont_box:
 
−
The containers (box) which hold the texts and imgs in the page.
 
−
 
−
img_cont:
 
−
Every in-content-page img needs to have this class of img.
 
−
 
−
no-title-col:
 
−
Every in-content-page img needs to have this class of col.
 
−
*/
 
−
.referances {
 
−
  font-size: 16px;
 
−
outline: 1px solid black;
 
−
padding: 20px;
 
−
}
 
−
 
−
/*canceling wiki bug (inlarge imgs stuck the page)*/
 
−
.modal-backdrop {
 
−
position: relative;
 
−
}
 
−
 
−
.ocenter .img-responsive {
 
−
margin: 0 auto;
 
−
}
 
−
 
−
.vcenter {
 
−
display: inline-block;
 
−
vertical-align: middle;
 
−
float: none;
 
−
}
 
−
 
−
.table{
 
−
font-family: "Candara", "Calibri", "Segoe", "Segoe UI", "Optima", Arial, sans-serif;
 
−
    font-size: 18px;
 
−
font-style: normal;
 
−
font-variant: normal;
 
−
font-weight: 400;
 
−
line-height: 27px;
 
−
 
−
}
 
−
 
−
 
−
.cir_tabs{
 
−
/*-webkit-filter: grayscale(0.75);*/
 
−
cursor: pointer;
 
−
transition: all .5s ease;
 
−
margin: 0 auto;
 
−
/* border: 2px solid #bbb;
 
−
box-shadow: 5px 5px 3px grey;
 
−
padding: 15px; */
 
−
}
 
−
 
−
.cir_tabs:hover{
 
−
-webkit-filter: grayscale(0);
 
−
transform: scale(1.2,1.2);
 
−
margin: 0 auto;
 
−
/*border: 2px solid #000;
 
−
box-shadow: 5px 5px 3px grey;*/
 
−
}
 
−
 
−
 
−
</style>
 
−
 
−
<style>/* inline page CSS */
 
−
 
−
body {
 
−
}
 
−
 
−
/* ==========Background and effects ========== */
 
−
/*Make sure the div is unuiqe to each page*/
 
−
.desk_wrapper {
 
−
position: relative;
 
−
background: white; /* For browsers that do not support gradients */
 
−
background: -webkit-linear-gradient(white, #ecf7fb, white); /* For Safari 5.1 to 6.0 */
 
−
background: -o-linear-gradient(white, #ecf7fb, white); /* For Opera 11.1 to 12.0 */
 
−
background: -moz-linear-gradient(white, #ecf7fb, white); /* For Firefox 3.6 to 15 */
 
−
background: linear-gradient(white, #ecf7fb, white); /* Standard syntax */
 
−
/*background-image:url('addres');
 
−
background-size:cover;
 
−
filter: blur(5px) grayscale(80%) opacity(10%);*/
 
−
}
 
−
/* ========== END: and effects ========== */
 
−
 
−
 
−
/*
 
−
Object in external CSS sheet:
 
−
 
−
nav-tabs, cont_tabs:
 
−
Open diffrent tabs, we uses imgs.
 
−
 
−
back-to-top:
 
−
go back to top. Apears only when going down the page.
 
−
 
−
cont_box:
 
−
The containers (box) which hold the texts and imgs in the page.
 
−
 
−
img_cont:
 
−
Every in-content-page img needs to have this class of img.
 
−
 
−
no-title-col:
 
−
Every in-content-page img needs to have this class of col.
 
−
*/
 
−
.referances {
 
−
  font-size: 16px;
 
−
outline: 1px solid black;
 
−
padding: 20px;
 
−
}
 
−
 
−
/*canceling wiki bug (inlarge imgs stuck the page)*/
 
−
.modal-backdrop {
 
−
position: relative;
 
−
}
 
−
 
−
.ocenter .img-responsive {
 
−
margin: 0 auto;
 
−
}
 
−
 
−
.vcenter {
 
−
display: inline-block;
 
−
vertical-align: middle;
 
−
float: none;
 
−
}
 
−
 
−
</style>
 
  
  
Line 243: Line 88:
 
<div class="col-md-6 col-sm-12">
 
<div class="col-md-6 col-sm-12">
 
<p class="text-justify">
 
<p class="text-justify">
−
<a href="https://static.igem.org/mediawiki/2016/6/6f/T--Technion_Israel--Agarose_Gel_Preparation.pdf" target="_blank">Agarose Gel Preparation</a>
+
<a href="https://static.igem.org/mediawiki/2016/6/6f/T--Technion_Israel--Agarose_Gel_Preparation.pdf" target="_blank">Agarose Gel Preparation</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/7/7c/T--Technion_Israel--Chemical_Transformation_protocol.pdf" target="_blank">Chemical Transformation</a>
+
<a href="https://static.igem.org/mediawiki/2016/7/7c/T--Technion_Israel--Chemical_Transformation_protocol.pdf" target="_blank">Chemical Transformation</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/1/11/T--Technion_Israel--Protocol_-_Chemotaxis_on_chip_test.pdf" target="_blank">Chemotaxis on chip test</a>
+
<a href="https://static.igem.org/mediawiki/2016/1/11/T--Technion_Israel--Protocol_-_Chemotaxis_on_chip_test.pdf" target="_blank">Chemotaxis on chip test</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/7/7c/T--Technion_Israel--Colony_PCR.pdf" target="_blank">Colony PCR</a>
+
<a href="https://static.igem.org/mediawiki/2016/7/7c/T--Technion_Israel--Colony_PCR.pdf" target="_blank">Colony PCR</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/f/fe/T--Technion_Israel--Protocol_Dpnl.pdf" target="_blank">DpnI</a>
+
<a href="https://static.igem.org/mediawiki/2016/f/fe/T--Technion_Israel--Protocol_Dpnl.pdf" target="_blank">DpnI</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/f/f3/T--Technion_Israel--Chemotaxis_assays.pdf#page=2" target="_blank">In plug assay</a>
+
<a href="https://static.igem.org/mediawiki/2016/7/77/T--Technion_Israel--PDMSprotocol.pdf" target="_blank">Fabrication of a PDMS chip</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/2/23/T--Technion_Israel--TarInteinincubationprotocol.pdf" target="_blank">Intein Incubation</a>
+
<a href="https://static.igem.org/mediawiki/2016/4/46/Gel_electrophoresis_protocol.pdf" target="_blank">Gel electrophoresis </a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/f/f9/T--Technion_Israel--0.1_M_potassium_phosphate_%28KPi%29.pdf" target="_blank">KPi Preparation</a>
+
<a href="https://static.igem.org/mediawiki/2016/1/14/T--Technion_Israel--Gel_Extraction_%28to_miniprep%29.pdf" target="_blank">Gel Extraction</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/7/77/T--Technion_Israel--PDMSprotocol.pdf" target="_blank">Fabrication of a PDMS chip</a>
+
<a href="https://static.igem.org/mediawiki/2016/c/ca/T--Technion_Israel--Gibson_assembly.pdf" target="_blank">Gibson Assembly</a>
−
<br><br>
+
−
<a href="https://static.igem.org/mediawiki/2016/4/46/Gel_electrophoresis_protocol.pdf" target="_blank">Gel electrophoresis </a>
+
 
</p>
 
</p>
 
</div>
 
</div>
Line 267: Line 111:
 
<div class="col-md-6 col-sm-12">
 
<div class="col-md-6 col-sm-12">
 
<p class="text-justify">
 
<p class="text-justify">
−
<a href="https://static.igem.org/mediawiki/2016/1/14/T--Technion_Israel--Gel_Extraction_%28to_miniprep%29.pdf" target="_blank">Gel Extraction</a>
+
<a href="https://static.igem.org/mediawiki/2016/d/dc/T--Technion_Israel--Glycerol_stock.pdf" target="_blank">Glycerol Stock</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/c/ca/T--Technion_Israel--Gibson_assembly.pdf" target="_blank">Gibson Assembly</a>
+
<a href="https://static.igem.org/mediawiki/2016/2/22/T--Technion_Israel--mediumprotocol.pdf" target="_blank">Growing medium assays</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/d/dc/T--Technion_Israel--Glycerol_stock.pdf" target="_blank">Glycerol Stock</a>
+
<a href="https://static.igem.org/mediawiki/2016/f/f3/T--Technion_Israel--Chemotaxis_assays.pdf#page=2" target="_blank">In plug assay</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/2/22/T--Technion_Israel--mediumprotocol.pdf" target="_blank">Growing medium assays</a><br><br>
+
<a href="https://static.igem.org/mediawiki/2016/2/23/T--Technion_Israel--TarInteinincubationprotocol.pdf" target="_blank">Intein Incubation</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/e/ef/T--Technion_Israel--PCR_protocol.pdf" target="_blank">PCR</a>
+
<a href="https://static.igem.org/mediawiki/2016/f/f9/T--Technion_Israel--0.1_M_potassium_phosphate_%28KPi%29.pdf" target="_blank">KPi Preparation</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/d/db/Restriction_protocol.pdf" target="_blank">Restriction and Ligation</a>
+
<a href="https://static.igem.org/mediawiki/2016/e/ef/T--Technion_Israel--PCR_protocol.pdf" target="_blank">PCR</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/c/ce/T--Technion_Israel--SOBfrozencompetentcellsprotocol.pdf" target="_blank">SOB frozen competent cells</a>
+
<a href="https://static.igem.org/mediawiki/2016/d/db/Restriction_protocol.pdf" target="_blank">Restriction and Ligation</a>
−
<br><br>
+
<br><br>
−
<a href="https://static.igem.org/mediawiki/2016/f/f3/T--Technion_Israel--Chemotaxis_assays.pdf" target="_blank">Swarming assay</a>
+
<a href="https://static.igem.org/mediawiki/2016/c/ce/T--Technion_Israel--SOBfrozencompetentcellsprotocol.pdf" target="_blank">SOB frozen competent cells</a>
 +
<br><br>
 +
<a href="https://static.igem.org/mediawiki/2016/f/f3/T--Technion_Israel--Chemotaxis_assays.pdf" target="_blank">Swarming assay</a>
 
</p>
 
</p>
  

Latest revision as of 00:47, 20 October 2016

S.tar, by iGEM Technion 2016

S.tar, by iGEM Technion 2016



Agarose Gel Preparation

Chemical Transformation

Chemotaxis on chip test

Colony PCR

DpnI

Fabrication of a PDMS chip

Gel electrophoresis

Gel Extraction

Gibson Assembly

Glycerol Stock

Growing medium assays

In plug assay

Intein Incubation

KPi Preparation

PCR

Restriction and Ligation

SOB frozen competent cells

Swarming assay




S.tar, by iGEM Technion 2016