Difference between revisions of "Team:Paris Saclay/Notebook/July/6"
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====Preparation of LB solid and liquid stock==== | ====Preparation of LB solid and liquid stock==== | ||
''By Léa, Naiane, Laetitia'' | ''By Léa, Naiane, Laetitia'' | ||
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- 2L of LB liquid : 20g/L powder LB + 2L water μQ | - 2L of LB liquid : 20g/L powder LB + 2L water μQ | ||
- 1L of LB solid : 1L of LB liquid +15g/L of Agar | - 1L of LB solid : 1L of LB liquid +15g/L of Agar | ||
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====Screen PCF for GBlock ==== | ====Screen PCF for GBlock ==== | ||
''By Caroline'' | ''By Caroline'' | ||
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<!--TODO : A relire ABSOLUMENT problème de lecture et de traduction--> | <!--TODO : A relire ABSOLUMENT problème de lecture et de traduction--> | ||
"Megamix" were prepared for 34 tubes with 70μL of 90xTaq buffer + 70 μL of <!-- dumpamM --> + 42 μL MgCl<SUB>2</SUB> 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.) | "Megamix" were prepared for 34 tubes with 70μL of 90xTaq buffer + 70 μL of <!-- dumpamM --> + 42 μL MgCl<SUB>2</SUB> 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.) | ||
Revision as of 21:53, 17 July 2016
