Difference between revisions of "Team:Paris Saclay/Notebook/July/6"
(→Lab work) |
(→Lab work) |
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''By Léa, Naiane, Laetitia'' | ''By Léa, Naiane, Laetitia'' | ||
| − | - 2L of LB liquid : 20g/L powder LB + 2L water μQ | + | - 2L of LB liquid : 20g/L powder LB + 2L water μQ<br/> |
| − | - 1L of LB solid : 1L of LB liquid +15g/L of Agar | + | - 1L of LB solid : 1L of LB liquid +15g/L of Agar<br/> |
The all was put in the autoclave for sterilization with the help of Sylvain. | The all was put in the autoclave for sterilization with the help of Sylvain. | ||
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<!--TODO : A relire ABSOLUMENT problème de lecture et de traduction--> | <!--TODO : A relire ABSOLUMENT problème de lecture et de traduction--> | ||
| − | "Megamix" were prepared for 34 tubes with 70μL of 90xTaq buffer + 70 μL of <!-- dumpamM --> + 42 μL MgCl<SUB>2</SUB> 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.) | + | "Megamix" were prepared for 34 tubes with 70μL of 90xTaq buffer + 70 μL of <!-- dumpamM --> + 42 μL MgCl<SUB>2</SUB> 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.)<br/> |
| − | 19 μL of mage was put into PCR's tubes then 1 μL of culture were added for each Gblock 1.1 1.2 2.1 2.2 3.1 and 3.2 and their 6 clones. (2 | + | 19 μL of mage was put into PCR's tubes then 1 μL of culture were added for each Gblock 1.1 1.2 2.1 2.2 3.1 and 3.2 and their 6 clones. (2 clones for 2.2). |
Revision as of 21:55, 17 July 2016
