Difference between revisions of "Team:Technion Israel/Composite Part"

 
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<img src="https://static.igem.org/mediawiki/2016/d/db/T--Technion_Israel--icon_intro.png" class="img-responsive img-center cir_tabs" width="75" style="cursor: pointer;">
 
</a>
 
</a>
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<h4 class="text-center"><b>Parts' Index</b></h4>
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<h4 class="text-center">Parts' Index</h4>
 
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<div class="row">
 
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<div class="col-md-12 col-sm-12">
 
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<h1>New composite part</h1>
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<h1>Best new composite part candidate</h1>
 
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</div>
 
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<p class="text-justify">
 
<p class="text-justify">
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This is our chemotaxis part collection. This We chose We chose Histamine-Tar receptor as our Best new composite part. The Histamine-Tar receptor was a novel design developed using a bioinformatic computational tool called Rosetta (link to the Rosetta page)  and shown to function as predicted (link to proof of concept) resulting in chemotaxis response to histamine as an attractant. This property is significant and can be used in various applications including detection for various foodstuff as histamine is known to be found in food gone bad, especially rotten fish.
+
We chose Histamine-Tar receptor as our Best new composite part. The Histamine-Tar receptor was a  
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This part is composed of strong promoter, Tar native RBS, Tar-Histamine (pop up:Tar LBD mutated to bind histamine)- part K1992002 (link to the part page: http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992002) and terminator.
+
novel design developed, using a bioinformatic computational tool, called  
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To learn more please check our Histamine-Tar part ,K1992011 (link to the part page: http://parts.igem.org/cgi/partsdb/part_info.cgi?part_name=BBa_K1992011#Page_Header), in the registry.
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<a href="https://2016.igem.org/Team:Technion_Israel/Modifications/Rosetta" target="_blank" style="cursor: pointer;">Rosetta</a>,
 +
and shown to  
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<a href="https://2016.igem.org/Team:Technion_Israel/Proof" target="_blank" style="cursor: pointer;">function as predicted</a>,
 +
resulting in chemotaxis response to histamine as an attractant. This property is important, and  
 +
can be used in various applications, including detection for various food products, as histamine is known
 +
to be found in spoiled food, especially rotten fish.<br>
 +
<br>
 +
This part is composed of strong promoter, Tar native RBS,  
 +
<a data-toggle="popover" data-trigger="click" data-original-title="Info:" style="cursor: pointer;"
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data-html="true" data-content="Tar's Ligand Binding Domain, mutated to bind histamine">
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Tar-Histamine<i class="entypo-check"></i></button></a>, 
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992002" target="_blank" style="cursor: pointer;">part K1992002</a>
 +
and a terminator.<br>
 +
<br>
 +
To learn more, please check our Histamine-Tar part,  
 +
<a href="http://parts.igem.org/cgi/partsdb/part_info.cgi?part_name=BBa_K1992011" target="_blank" style="cursor: pointer;">K1992011</a>,  
 +
in the registry.
 
<br>
 
<br>
 
</p>
 
</p>
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</div>
 
</div>
  
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<br>
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</div>
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<br>
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</div>
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<br>
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</div>
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<div class="row"><!--row -->
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<div class="col-sm-8 col-sm-offset-2"><!-- 10/12 -->
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<h3>
 +
Full list of our composite parts:
 +
</h3>
  
 +
    <table class="table table-list-search">
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                    <thead>
 +
                        <tr>
 +
                            <th> </th>
 +
                            <th> </th>
 +
                            <th> </th>
 +
                            <th>Name</th>
 +
                            <th>Type</th>
 +
                            <th>Description</th>
 +
                            <th>Designer</th>
 +
                            <th>Length</th>
 +
                        </tr>
 +
                    </thead>
 +
                    <tbody>
 +
                        <tr>
 +
                            <td>Favorite</td>
 +
                            <td></td>
 +
                            <td>W</td>
 +
                            <td>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992005" target="_blank">BBa_K1992005</a>
 +
</td>
 +
                            <td>Composite</td>
 +
                            <td>Tar native RBS expression system (promoter+ RBS+ coding+ terminator)</td>
 +
                            <td>Tzila Davidov, Shilo Ohayon</td>
 +
<td>1872</td>
 +
                        </tr>
  
 +
                        <tr>
 +
                            <td>Favorite</td>
 +
                            <td></td>
 +
                            <td>W</td>
 +
                            <td>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992011" target="_blank">BBa_K1992011</a>
 +
</td>
 +
                            <td>Composite</td>
 +
                            <td>Histamin-Tar expression system(promoter+ RBS+ coding+ terminator)</td>
 +
                            <td>Shiran Sarig, Inbal Adir</td>
 +
<td>1872</td>
 +
                        </tr>
  
 +
                        <tr>
 +
                            <td></td>
 +
                            <td></td>
 +
                            <td>W</td>
 +
                            <td>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992004" target="_blank">BBa_K1992004</a>
 +
</td>
 +
                            <td>Composite</td>
 +
                            <td>Tar expression system (promoter+ RBS+ coding+ terminator)</td>
 +
                            <td>Tzila Davidov</td>
 +
<td>1860</td>
 +
                        </tr>
  
 +
                        <tr>
 +
                            <td></td>
 +
                            <td>U</td>
 +
                            <td>W</td>
 +
                            <td>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992006" target="_blank">BBa_K1992006</a>
 +
</td>
 +
                            <td>Composite</td>
 +
                            <td>Histamin-Tar GFP tagged expression system (promoter+ RBS+ coding+ terminator)</td>
 +
                            <td>Inbal Adir, Shilo Ohayon</td>
 +
<td>2624</td>
 +
                        </tr>
 +
 +
                        <tr>
 +
                            <td></td>
 +
                            <td></td>
 +
                            <td>W</td>
 +
                            <td>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992007" target="_blank">BBa_K1992007</a>
 +
</td>
 +
                            <td>Composite</td>
 +
                            <td>PctA-Tar expression system (promoter+ RBS+ coding+ terminator)</td>
 +
                            <td>Shilo Ohayon, Tzila Davidov</td>
 +
<td>2118</td>
 +
                        </tr>
 +
 +
                        <tr>
 +
                            <td></td>
 +
                            <td></td>
 +
                            <td>W</td>
 +
                            <td>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992008" target="_blank">BBa_K1992008</a>
 +
</td>
 +
                            <td>Composite</td>
 +
                            <td>Tar GFP tagged, native RBS expression system (promoter+ RBS+ coding+ terminator)</td>
 +
                            <td>Shilo Ohayon, Sharbel Zahran</td>
 +
<td>2610</td>
 +
                        </tr>
 +
 +
                        <tr>
 +
                            <td></td>
 +
                            <td></td>
 +
                            <td>W</td>
 +
                            <td>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992009" target="_blank">BBa_K1992009</a>
 +
</td>
 +
                            <td>Composite</td>
 +
                            <td>Tar GFP tagged expression system (promoter+ RBS+ coding+ terminator)</td>
 +
                            <td>Shilo Ohayon, Sharbel Zahran</td>
 +
<td>2598</td>
 +
                        </tr>
 +
 +
                        <tr>
 +
                            <td></td>
 +
                            <td></td>
 +
                            <td>W</td>
 +
                            <td>
 +
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1992010" target="_blank">BBa_K1992010</a>
 +
</td>
 +
                            <td>Composite</td>
 +
                            <td>promoter+RBS+PctA-tar hybrid fused w GFP+terminator</td>
 +
                            <td>Shilo Ohayon</td>
 +
<td>2870</td>
 +
                        </tr>
 +
 +
                    </tbody>
 +
                </table> 
  
 
 
 
</div>
 
</div>
 
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 +
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</div>
 
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Latest revision as of 02:38, 20 October 2016

S.tar, by iGEM Technion 2016

S.tar, by iGEM Technion 2016

Parts' Index


Best new composite part candidate

We chose Histamine-Tar receptor as our Best new composite part. The Histamine-Tar receptor was a novel design developed, using a bioinformatic computational tool, called Rosetta, and shown to function as predicted, resulting in chemotaxis response to histamine as an attractant. This property is important, and can be used in various applications, including detection for various food products, as histamine is known to be found in spoiled food, especially rotten fish.

This part is composed of strong promoter, Tar native RBS, Tar-Histamine, part K1992002 and a terminator.

To learn more, please check our Histamine-Tar part, K1992011, in the registry.

Full list of our composite parts:

Name Type Description Designer Length
Favorite W BBa_K1992005 Composite Tar native RBS expression system (promoter+ RBS+ coding+ terminator) Tzila Davidov, Shilo Ohayon 1872
Favorite W BBa_K1992011 Composite Histamin-Tar expression system(promoter+ RBS+ coding+ terminator) Shiran Sarig, Inbal Adir 1872
W BBa_K1992004 Composite Tar expression system (promoter+ RBS+ coding+ terminator) Tzila Davidov 1860
U W BBa_K1992006 Composite Histamin-Tar GFP tagged expression system (promoter+ RBS+ coding+ terminator) Inbal Adir, Shilo Ohayon 2624
W BBa_K1992007 Composite PctA-Tar expression system (promoter+ RBS+ coding+ terminator) Shilo Ohayon, Tzila Davidov 2118
W BBa_K1992008 Composite Tar GFP tagged, native RBS expression system (promoter+ RBS+ coding+ terminator) Shilo Ohayon, Sharbel Zahran 2610
W BBa_K1992009 Composite Tar GFP tagged expression system (promoter+ RBS+ coding+ terminator) Shilo Ohayon, Sharbel Zahran 2598
W BBa_K1992010 Composite promoter+RBS+PctA-tar hybrid fused w GFP+terminator Shilo Ohayon 2870
S.tar, by iGEM Technion 2016