Difference between revisions of "Team:Freiburg/Lab"

 
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        <h5 style="text-align: center"> Lab Summary </h5>
 
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            <center><img align="right" style="width:350px" src="https://static.igem.org/mediawiki/2016/c/c3/T--Freiburg--Lab0004.png"> </center><font color="#74e4f7">
 
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This year our team was working with the model organisms <i>Bacillus subtilis</i> and <i>Escherichia coli</i>. Since <i>E. coli</i> is commonly used in many laboratories, we had no problem handling it. Apparently, there are only a few people who are working with <i>Bacillus subtilis</i>, but we kindly received some help and protocols from former iGEM team Munich 2012 and support from the Bacillus Genetic Stock Center (BGSC) from Ohio who always answered our numerous questions. <font color="#74e4f7">
 
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We want to use the endospores of <i>Bacillus subtilis</i> as a carrier vessel for a prodrug activating enzyme and a nanobody to improve the targeted drug delivery system.
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<i>“This is my lab. There are many like it, but this one is mine.
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My lab is my best friend. It is my life. I must master it as I must master my life.
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Without me, my lab is useless. Without my lab, I am useless. I must work at my bench true. I must work harder than my fellow student who is trying to outdo me. I must leave after he leaves.
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My lab and I know that what counts in science is not the sweat, the noise of our burst, nor the smoke we make. We know that it is the result that counts.
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My lab is human, even as I, because it is my life. Thus, I will learn it as a brother. I will learn its weaknesses, its strength, its parts, its accessories, its sights and its content. I will keep my lab clean and ready, even as I am clean and ready. We will become part of each other.“</i>
  
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Without a lab, there is no iGEM!! Luckily we  had  the opportunity to work in the lab of our main sponsor, the BIOSS. They didn't just provide the lab but also a lot of their professional equipment like the FACS, the fluorescence microscope and personal support. They kindly taught us the handling of the equipment.
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Without the BIOSS our great project would not have been possible.
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Fortunately, they also provided us access to the kitchen of the institute. Otherwise, possibly the half of our team members would have been starving or dying of
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caffeine lack :). 
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<i>Bacillus subtilis</i> builds these spores under distressed conditions (such as nutrition deficiency). The spores are highly resistant to heat, UV-light and are easy to handle.
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We modified the spores in their coat proteins so that they are expressing the fusion proteins, the Glutathione S-Transferase (GST) and nanobody, on their surface. We experimented with modifications on the genes CotZ, CgeA,CotG and CotB, to see where we could achieve the optimal conditions.
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Latest revision as of 02:55, 20 October 2016

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“This is my lab. There are many like it, but this one is mine. My lab is my best friend. It is my life. I must master it as I must master my life. Without me, my lab is useless. Without my lab, I am useless. I must work at my bench true. I must work harder than my fellow student who is trying to outdo me. I must leave after he leaves. My lab and I know that what counts in science is not the sweat, the noise of our burst, nor the smoke we make. We know that it is the result that counts. My lab is human, even as I, because it is my life. Thus, I will learn it as a brother. I will learn its weaknesses, its strength, its parts, its accessories, its sights and its content. I will keep my lab clean and ready, even as I am clean and ready. We will become part of each other.“
Without a lab, there is no iGEM!! Luckily we had the opportunity to work in the lab of our main sponsor, the BIOSS. They didn't just provide the lab but also a lot of their professional equipment like the FACS, the fluorescence microscope and personal support. They kindly taught us the handling of the equipment. Without the BIOSS our great project would not have been possible. Fortunately, they also provided us access to the kitchen of the institute. Otherwise, possibly the half of our team members would have been starving or dying of caffeine lack :).


Posted by: iGEM Freiburg

Nanocillus - 'cause spore is more!