Difference between revisions of "Team:Newcastle/Notebook/Lab"

 
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<h2 id="20/06/16">20/06/16</h2>
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<p>After the interlab study, we made streak plates from the colonies we had grown. We regrew all the samples on LB agar with 1 in 1000 dilution of Chloramphenicol. We did this to isolate a pure strain of the transformed interlab E. coli, therefore allowing us to grow up a new, genetically-identical plate. Our lab supervisor, Matthew Peake, showed us the correct streaking technique as the Computer Science students had not learnt this technique before.
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<p>After analysing the trial interlab results, we decided to re-plate up the positive control to ensure that we would have enough colonies to carry out the interlab study again.
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<p>Today we made a microbial fuel cell by following the Reading University’s protocol, see below.
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<p>We sourced the material such as the neoprene gaskets, carbon fibre electrode material, cation-exchange membrane, J-cloth from Professor Ian Head, Dr. Ed Milner and Paniz Izadi from the School of Civil Engineering and Geosciences. We also sourced electric wires with crocodile clips and a multimeter from the Engineering Departments.
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<p>First, we prepared the 1M glucose solution, 0.02M potassium hexacyanoferrate (III) solution, 10mM methylene blue solution, these were made up in a 0.1M potassium phosphate buffer.
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<h3>Lab Notes</h3>
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<p> In the links presented below are the lab diaries for each individual team.</p>
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<h2><a href="https://2016.igem.org/Team:Newcastle/Notebook/Lab/AlternativeConstructs">Alternative Constructs Diary</a></h2>
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<h2><a href="https://2016.igem.org/Team:Newcastle/Notebook/Lab/Lightbulb">Lightbulb Diary</a></h2>
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<h2><a href="https://2016.igem.org/Team:Newcastle/Notebook/Lab/Microfluidics">Microfluidics Diary</a></h2>
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<h2><a href="https://2016.igem.org/Team:Newcastle/Notebook/Lab/Component">Measuring GFP Expression using off-the-shelf Hardware Write-up</a></h2>
  
 
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Latest revision as of 03:20, 20 October 2016

Lab Notes

In the links presented below are the lab diaries for each individual team.

Alternative Constructs Diary

Lightbulb Diary

Microfluidics Diary

Measuring GFP Expression using off-the-shelf Hardware Write-up