Difference between revisions of "Team:Freiburg/Results"

Revision as of 07:15, 18 October 2016 (view source) Nwagner (Talk | contribs) ← Older edit		Latest revision as of 03:26, 20 October 2016 (view source) D.Steinberg (Talk | contribs)
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	<div class="column full_size" >		<div class="column full_size" >
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	<h5  style="text-align: center"> Overview </h5>		<h5  style="text-align: center"> Overview </h5>
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−	version="1.1"	+	<a target="_blank" style="color: #66ccff" href="https://2016.igem.org/Team:Freiburg/Proof">“Eliminate all other factors, and the one which remains must be the truth.”</a> <br> – The Sign of Four
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−	<tspan x="500" y="600">Carrier - Using the endospores of Bacillus subtilis as a</tspan>
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−	<tspan x="500" y="600">Drug Delivery - Glutathione-S-Transferase is an</tspan>
−	<tspan x="500" y="630">enzyme that is responsible for several detoxification reactions</tspan>
−	<tspan x="500" y="660">in the liver. We use it locally to convert an inactive prodrug</tspan>
−	<tspan x="500" y="690">into the active drug. </tspan>
		+	<a target="_blank" style="color: #66ccff" href="https://2016.igem.org/Team:Freiburg/Binding">Roses are red violets are blue I am just a nanobody waiting for you</a>
		+	<br>
		+	<br>
		+
		+	<a target="_blank" style="color: #66ccff" href="https://2016.igem.org/Team:Freiburg/Delivery">They call themselves the "Delivery Boys"</a>
		+	<br>
		+	<br>
		+
		+	<a target="_blank" style="color: #66ccff" href="https://2016.igem.org/Team:Freiburg/Production_Nanocillus"> A new hope </a>
		+	<br>
		+	<br>
		+
		+	<a target="_blank" style="color: #66ccff" href="https://2016.igem.org/Team:Freiburg/Biobricks">
		+	Not just another brick in the wall </a>
		+	<br>
		+	<br>
		+
		+	<a target="_blank" style="color: #66ccff" href="https://2016.igem.org/Team:Freiburg/Shampoo"> Click for a frothy surprise </a>
		+	<br>
		+	<br>
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−	<tspan x="500" y="600">The Binding - The Nanobody, a single domain antibody,</tspan>
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−	<tspan x="500" y="600">Glutathione S-Transferase forms the reaction from</tspan>
−	<tspan x="500" y="630">the inactive prodrug azathioprine to the active 6-Mercaptopurine, </tspan>
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−	</div>
−	<div class="color6">	+
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−	The treatment of diseases while avoiding systemic side effects is still a major obstacle in modern medicine. After administration, conventional drugs are distributed throughout the whole body thus affecting both, diseased and healthy cells. Current strategies on targeted drug delivery are mainly based on the applications of antibody-drug conjugates or nanoparticles. However, both approaches revealed considerable challenges in their application due to short half-life and expensive production, respectively. We develop a novel platform for targeted drug delivery by implementing highly specific nanobodies directed against surface markers of affected cells. The combination with an enzymatic functionality facilitates the local activation of prodrugs, thus preventing unnecessary side effects by systemic drug dispersal. By engineering the spores of probiotic Bacillus subtilis, a member of the human microbiome, we establish a low-cost carrier for well-tolerated treatment.	+
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Latest revision as of 03:26, 20 October 2016

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