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<strong> Biology</strong></h2>
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<h5>Advice on writing your Project Description</h5>
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  <p>
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    <strong>Improvement: logic gate genetic components
 +
    </strong></p>
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  <p>
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Previous Parts: BBa_K137008+BBa_K137010, BBa_J11032+BBa_J11033
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Improved Part: BBa_K2041016
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<br><br> </p>
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<div align="center"><img src="https://static.igem.org/mediawiki/2016/8/8b/BIT_composite_part.jpg" class="img-responsive"> <br><br></div>
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<center> <font size="1px"> Figure: logic gate genetic components</font></center><br><br>  
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<p>
 
<p>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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Compared with the previous parts, we combine the individual
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</p>
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parts and design the double integrases site in order to make it possible for joint determination. And the part form an AND gate, which shows the probability of making up more complex combination of logic gates. To the best of our knowledge, it is the first time of the double integrases system to be introduced in the field of early diagnosis of breast cancer by the simultaneous determination of two kinds of microRNA.
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<br><br></p>  
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<p>
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<strong>Innovation 1: ribosomal switch (Lock & Key)
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</strong><br><br></p> 
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<div align="center"><img src="https://static.igem.org/mediawiki/2016/f/f4/2016.igem.orgwikiimages9BIT.jpg" class="img-responsive"> <br><br></div> 
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<center> <font size="1px"> Figure:ribosomal switch (Lock & Key)</font></center><br><br>
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<p>
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Employing the Lock & Key part in our system reduce the background noise in order to achieve the lower limit of detection.
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<br><br></p>
 +
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<p>
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<strong>Innovation 2: RNA-based gene silencing expression </strong><br><br></p>
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<div align="center"><img src="https://static.igem.org/mediawiki/2016/4/4a/2016.igem.orgwikiimages8BIT.jpg" class="img-responsive"> <br><br></div>
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<center> <font size="1px"> Figure:RNA-based gene silencing expression</font></center><br><br>
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<p>
 
<p>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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The features of gene silence make the detection of microRNA possible and effective in the engineering bacteria.
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    <div><h2><br><br><br><br><br><br><br>
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                        <strong> Hardware</strong></h2>
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    <P>
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we provide a low cost and non-laboratory hardware solution for pre-disease detection. Most of our components are based on standard open source hardware. Different from last year’s project, we have integrated cultivation and detection functions on one instrument, and make it work automatically which can make the detection more rapid, cheap and convenient.
 +
    <br><br></P>
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      <div align="center"><img src="https://static.igem.org/mediawiki/2016/0/00/BIT_last_year_device.jpg" class="img-responsive"> <br><br></div>
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      <center> <font size="1px">Figure The device designed last year (left is culturing device and right is detection device)</font></center><br><br>
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      <p>
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In cultivation part, we developed a close loop thermostat system with Multi-point temperature measuring and PID temperature control algorithm, based on low cost open source hardware.
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    <br><br></P>
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    <div align="center"><img src="https://static.igem.org/mediawiki/2016/3/38/BIT_Figure_Hardware_Internal_Design.png" class="img-responsive"> <br><br></div>
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      <center> <font size="1px">Figure The device designed this year </font></center><br><br>
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    <p>
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As for detection sensor, we used CMOS camera with better linearity instead of the old monolithic photodiode, providing higher detection accuracy. For measuring consistency, we used sliding table to build a mobile platform, achieved point-by-point detection, with can also improve detection accuracy.
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    <br><br><br><br><br><br><br><br><br><br><br><br></P>
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Beijing Institute of Technology,<br>
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No. 5 South Zhong Guan Cun Street,<br>
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Haidian Beijing 100081, P. R. China<br><br>
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Twitter : @igem_BIT<br><br>
  
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Sina Weibo : @igem_BIT<br><br>
  
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Website : http://www.bit.edu.cn
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Latest revision as of 03:33, 20 October 2016

<!DOCTYPE html> Biology

ACHIEVEMENT

DESCRIPTION








Description








Biology

Improvement: logic gate genetic components

Previous Parts: BBa_K137008+BBa_K137010, BBa_J11032+BBa_J11033 Improved Part: BBa_K2041016



Figure: logic gate genetic components


Compared with the previous parts, we combine the individual parts and design the double integrases site in order to make it possible for joint determination. And the part form an AND gate, which shows the probability of making up more complex combination of logic gates. To the best of our knowledge, it is the first time of the double integrases system to be introduced in the field of early diagnosis of breast cancer by the simultaneous determination of two kinds of microRNA.

Innovation 1: ribosomal switch (Lock & Key)



Figure:ribosomal switch (Lock & Key)


Employing the Lock & Key part in our system reduce the background noise in order to achieve the lower limit of detection.

Innovation 2: RNA-based gene silencing expression



Figure:RNA-based gene silencing expression


The features of gene silence make the detection of microRNA possible and effective in the engineering bacteria.








Hardware

we provide a low cost and non-laboratory hardware solution for pre-disease detection. Most of our components are based on standard open source hardware. Different from last year’s project, we have integrated cultivation and detection functions on one instrument, and make it work automatically which can make the detection more rapid, cheap and convenient.



Figure The device designed last year (left is culturing device and right is detection device)


In cultivation part, we developed a close loop thermostat system with Multi-point temperature measuring and PID temperature control algorithm, based on low cost open source hardware.



Figure The device designed this year


As for detection sensor, we used CMOS camera with better linearity instead of the old monolithic photodiode, providing higher detection accuracy. For measuring consistency, we used sliding table to build a mobile platform, achieved point-by-point detection, with can also improve detection accuracy.











Contact Us

Address

Beijing Institute of Technology,
No. 5 South Zhong Guan Cun Street,
Haidian Beijing 100081, P. R. China

Twitter : @igem_BIT

Sina Weibo : @igem_BIT

Website : http://www.bit.edu.cn
Send