Difference between revisions of "Team:BIT/Description"

 
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<li><a href="https://2016.igem.org/Team:BIT/Judging_Form" >Judging</a></li>
 
<li><a href="https://2016.igem.org/Team:BIT/Judging_Form" >Judging</a></li>
 
                 <li><a href="https://2016.igem.org/Team:BIT/Parts_Submission">Parts</a></li>
 
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                 <li><a href="https://2016.igem.org/Team:BIT/HP/Silver"><span class="style1">Attrievement</span></a></li>
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                 <li><a href="https://2016.igem.org/Team:BIT/HP/Silver"><span class="style1">Achievement</span></a></li>
 
<li><a href="https://2016.igem.org/Team:BIT/Introduction">Team</a></li>
 
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     <li><a href="https://2016.igem.org/Team:BIT/HP/Silver"><h2>HP/Silver</h2></a></li>
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     <li class=color1><a href="https://2016.igem.org/Team:BIT/Description"><h2>Description</h2></a></li>
 
     <li class=color1><a href="https://2016.igem.org/Team:BIT/Description"><h2>Description</h2></a></li>
 
     <li><a href="https://2016.igem.org/Team:BIT/Proof"><h2>Proof</h2></a></li>
 
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                 <div id="words" style="float:right;width:70%">
 
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<h2><br><br><br><br><br><br><br>Model</h2>
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  <h2><br><br><br><br><br><br><br>
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<strong> Description</strong></h2>
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Chinese Premier Li Keqiang delivers a keynote speech on October 11 at the opening ceremony of the Fifth Ministerial Conference of The Forum for Economic and Trade Cooperation Between China and Portuguese-speaking Countries in Macao. [Photo/Xinhua]<br><br></p>
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<div><h2><br><br><br><br><br><br><br>
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<strong> Biology</strong></h2>
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  <p>
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    <strong>Improvement: logic gate genetic components
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Previous Parts: BBa_K137008+BBa_K137010, BBa_J11032+BBa_J11033
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Improved Part: BBa_K2041016
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<br><br> </p>
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<div align="center"><img src="https://static.igem.org/mediawiki/2016/8/8b/BIT_composite_part.jpg" class="img-responsive"> <br><br></div>
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<center> <font size="1px"> Figure: logic gate genetic components</font></center><br><br>  
 
   
 
   
 
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Premier Li Keqiang announced 18 measures on Tuesday in Macao to boost ties with Portuguese-speaking countries in fields such as production capacity, people-to-people exchanges and maritime development.<br><br>
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Compared with the previous parts, we combine the individual
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parts and design the double integrases site in order to make it possible for joint determination. And the part form an AND gate, which shows the probability of making up more complex combination of logic gates. To the best of our knowledge, it is the first time of the double integrases system to be introduced in the field of early diagnosis of breast cancer by the simultaneous determination of two kinds of microRNA.
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<br><br></p>
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<p>
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<strong>Innovation 1: ribosomal switch (Lock & Key)
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</strong><br><br></p> 
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<div align="center"><img src="https://static.igem.org/mediawiki/2016/f/f4/2016.igem.orgwikiimages9BIT.jpg" class="img-responsive"> <br><br></div> 
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<center> <font size="1px"> Figure:ribosomal switch (Lock & Key)</font></center><br><br>
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<p>
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Employing the Lock & Key part in our system reduce the background noise in order to achieve the lower limit of detection.
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<br><br></p>  
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<p>
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<strong>Innovation 2: RNA-based gene silencing expression </strong><br><br></p>  
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<div align="center"><img src="https://static.igem.org/mediawiki/2016/4/4a/2016.igem.orgwikiimages8BIT.jpg" class="img-responsive"> <br><br></div>
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<center> <font size="1px"> Figure:RNA-based gene silencing expression</font></center><br><br>
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<p>
 
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The measures will be implemented in the next three years, and the Chinese government will support the Macao Special Administrative Region in establishing a financial service program, cultural exchange centers, bilingual training bases and juvenile innovation centers for exchanges with these countries, the premier said.<br><br>
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The features of gene silence make the detection of microRNA possible and effective in the engineering bacteria.
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China resumed sovereignty over Macao in December 1999 after it had been ruled since the mid-16th century by Portugal.<br><br>
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Beijing is willing to carry out major projects with these countries by making use of the China-Portuguese-Speaking Countries Cooperation Development Fund and the Silk Road Fund, Li said.<br><br>
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                        <strong> Hardware</strong></h2>
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The announcement was made in Li's keynote speech on Tuesday at the opening ceremony of the two-day Fifth Ministerial Conference of the Forum for Economic and Trade Cooperation Between China and Portuguese-Speaking Countries.<br><br>
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</p>
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we provide a low cost and non-laboratory hardware solution for pre-disease detection. Most of our components are based on standard open source hardware. Different from last year’s project, we have integrated cultivation and detection functions on one instrument, and make it work automatically which can make the detection more rapid, cheap and convenient.
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Delegates from seven Portuguese speaking countries — Portugal, Brazil, Cape Verde, Guinea-Bissau, Mozambique, Angola and East Timor — attended the forum.<br><br>
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      <div align="center"><img src="https://static.igem.org/mediawiki/2016/0/00/BIT_last_year_device.jpg" class="img-responsive"> <br><br></div>
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      <center> <font size="1px">Figure The device designed last year (left is culturing device and right is detection device)</font></center><br><br>
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Before the ceremony, Li launched a trade and economic cooperation program to promote further exchanges between China and Portuguese-speaking countries.
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      <p>
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In cultivation part, we developed a close loop thermostat system with Multi-point temperature measuring and PID temperature control algorithm, based on low cost open source hardware.
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    <br><br></P>
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China and the Portuguese-speaking countries contribute 17 percent of the world economy and 22 percent of global population, with varying advantages in technology, capital and market practices, Li said.<br><br>
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      <center> <font size="1px">Figure The device designed this year </font></center><br><br>
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The participating countries should consolidate political trust, promote trade liberalization and investment and strengthen cooperation in production capacity and people-to-people exchanges, the premier told the forum.<br><br>
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<img src="https://static.igem.org/mediawiki/2016/1/1a/BIT_test.jpg" class="img-responsive" alt="...">
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As for detection sensor, we used CMOS camera with better linearity instead of the old monolithic photodiode, providing higher detection accuracy. For measuring consistency, we used sliding table to build a mobile platform, achieved point-by-point detection, with can also improve detection accuracy.
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China's economic restructuring and upgrading will bring great investment opportunities and market potential, creating bright prospects for businesses from Portuguese-speaking countries, Li said.<br><br>
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He said the trade volume between China and Portuguese-speaking countries reached $100 billion last year — about 10 times of that of 2003.<br><br>
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Cape Verde's Prime Minister Ulisses Correia e Silva said the Macao forum injected new momentum into strategic cooperation between all the participating countries. He added that his country will align with China's Belt and Road Initiative and make use of the Macao forum to bring more commodities to Africa.<br><br>
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The prime ministers of Guinea-Bissau and Mozambique vowed to adopt favorable policies and improve the rule of law to attract investors.<br><br>
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Deng Yanzi contributed to this story<br><br><br><br><br><br>
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<h3>iGEM is a Jamboree! </h3>
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<h6>Wang Xu</h6>
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Latest revision as of 03:33, 20 October 2016

<!DOCTYPE html> Biology

ACHIEVEMENT

DESCRIPTION








Description








Biology

Improvement: logic gate genetic components

Previous Parts: BBa_K137008+BBa_K137010, BBa_J11032+BBa_J11033 Improved Part: BBa_K2041016



Figure: logic gate genetic components


Compared with the previous parts, we combine the individual parts and design the double integrases site in order to make it possible for joint determination. And the part form an AND gate, which shows the probability of making up more complex combination of logic gates. To the best of our knowledge, it is the first time of the double integrases system to be introduced in the field of early diagnosis of breast cancer by the simultaneous determination of two kinds of microRNA.

Innovation 1: ribosomal switch (Lock & Key)



Figure:ribosomal switch (Lock & Key)


Employing the Lock & Key part in our system reduce the background noise in order to achieve the lower limit of detection.

Innovation 2: RNA-based gene silencing expression



Figure:RNA-based gene silencing expression


The features of gene silence make the detection of microRNA possible and effective in the engineering bacteria.








Hardware

we provide a low cost and non-laboratory hardware solution for pre-disease detection. Most of our components are based on standard open source hardware. Different from last year’s project, we have integrated cultivation and detection functions on one instrument, and make it work automatically which can make the detection more rapid, cheap and convenient.



Figure The device designed last year (left is culturing device and right is detection device)


In cultivation part, we developed a close loop thermostat system with Multi-point temperature measuring and PID temperature control algorithm, based on low cost open source hardware.



Figure The device designed this year


As for detection sensor, we used CMOS camera with better linearity instead of the old monolithic photodiode, providing higher detection accuracy. For measuring consistency, we used sliding table to build a mobile platform, achieved point-by-point detection, with can also improve detection accuracy.











Contact Us

Address

Beijing Institute of Technology,
No. 5 South Zhong Guan Cun Street,
Haidian Beijing 100081, P. R. China

Twitter : @igem_BIT

Sina Weibo : @igem_BIT

Website : http://www.bit.edu.cn
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