Difference between revisions of "Team:BIT/Basic Part"

 
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<li><a href="https://2016.igem.org/Team:BIT/Judging_Form" >Judging</a></li>
 
<li><a href="https://2016.igem.org/Team:BIT/Judging_Form" >Judging</a></li>
 
                 <li><a href="https://2016.igem.org/Team:BIT/Parts_Submission"><span class="style1">Parts</span></a></li>
 
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                 <li><a href="https://2016.igem.org/Team:BIT/HP/Silver">Attrievement</a></li>
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                 <li><a href="https://2016.igem.org/Team:BIT/HP/Silver">Achievement</a></li>
 
<li><a href="https://2016.igem.org/Team:BIT/Introduction">Team</a></li>
 
<li><a href="https://2016.igem.org/Team:BIT/Introduction">Team</a></li>
 
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<p><b>miRNA-155 binding site</b></p>
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<center> <font size="1px">miRNA-155 binding site</font></center><br><br>
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<p> MiRNAs are widely recognized as biomarkers of many diseases. Many studies have shown that, miRNA-155 in patients with breast cancer in the body was significantly higher than normal people. After a thorough literature review , we know the recognition site of miRNA-155, of which  the transcription product, mRNA and miRNA-155 base-specific complementary matching, in order to increase its binding specificity with miRNA-155, we design four binding sites for this part,  so that when the miRNA-155 presence, it can be quickly identified by this fragment and combine to form double-stranded mRNA complex, blocking the binding of ribosomes, which will inhibit the downstream gene expression, which is called RNA-based gene silencing expression. This can serve as a switch to regulate the expression of downstream genes.
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<p>MiRNAs are widely recognized as biomarkers of many diseases. Many studies have shown that, miRNA-155 in patients with breast cancer in the body was significantly higher than normal people. After a thorough literature review , we know the recognition site of miRNA-155, of which  the transcription product, mRNA and miRNA-155 base-specific complementary matching, in order to increase its binding specificity with miRNA-155, we design four binding sites for this part,  so that when the miRNA-155 presence, it can be quickly identified by this fragment and combine to form double-stranded mRNA complex, blocking the binding of ribosomes, which will inhibit the downstream gene expression, which is called RNA-based gene silencing expression. This can serve as a switch to regulate the expression of downstream genes.
 
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<h3>iGEM is a Jamboree! </h3>
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<h6>Wang Xu</h6>
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Latest revision as of 03:36, 20 October 2016

<!DOCTYPE html> Biology

PARTS

Basic Part








Basic Part


...
miRNA-155 binding site


MiRNAs are widely recognized as biomarkers of many diseases. Many studies have shown that, miRNA-155 in patients with breast cancer in the body was significantly higher than normal people. After a thorough literature review , we know the recognition site of miRNA-155, of which the transcription product, mRNA and miRNA-155 base-specific complementary matching, in order to increase its binding specificity with miRNA-155, we design four binding sites for this part, so that when the miRNA-155 presence, it can be quickly identified by this fragment and combine to form double-stranded mRNA complex, blocking the binding of ribosomes, which will inhibit the downstream gene expression, which is called RNA-based gene silencing expression. This can serve as a switch to regulate the expression of downstream genes.






Contact Us

Address

Beijing Institute of Technology,
No. 5 South Zhong Guan Cun Street,
Haidian Beijing 100081, P. R. China

Twitter : @igem_BIT

Sina Weibo : @igem_BIT

Website : http://www.bit.edu.cn
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