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| | {{Missouri_Rolla}} | | {{Missouri_Rolla}} |
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| | <a href="https://2016.igem.org/Team:Missouri_Rolla"><img id="logo2" src="https://static.igem.org/mediawiki/2016/d/d0/T--Missouri_Rolla--Logo_2016_red.png" /><img id="logo1" src="https://static.igem.org/mediawiki/2016/e/ea/T--Missouri_Rolla--Logo_2016.png" /></a> | | <a href="https://2016.igem.org/Team:Missouri_Rolla"><img id="logo2" src="https://static.igem.org/mediawiki/2016/d/d0/T--Missouri_Rolla--Logo_2016_red.png" /><img id="logo1" src="https://static.igem.org/mediawiki/2016/e/ea/T--Missouri_Rolla--Logo_2016.png" /></a> |
| | <div id="menu"> | | <div id="menu"> |
| | <a href="https://2016.igem.org/Team:Missouri_Rolla">Home</a> | | <a href="https://2016.igem.org/Team:Missouri_Rolla">Home</a> |
| − | <a href="https://2016.igem.org/Team:Missouri_Rolla">Project</a> | + | <a href="https://2016.igem.org/Team:Missouri_Rolla/Project">Project</a> |
| − | <a href="https://2016.igem.org/Team:Missouri_Rolla">Human Practices</a> | + | <a href="https://2016.igem.org/Team:Missouri_Rolla/Engagement">Human Practices</a> |
| − | <a href="https://2016.igem.org/Team:Missouri_Rolla">Parts</a> | + | <a href="https://2016.igem.org/Team:Missouri_Rolla/Notebook">Notebook</a> |
| − | <a href="https://2016.igem.org/Team:Missouri_Rolla">Notebook</a>
| + | |
| | <a href="https://2016.igem.org/Team:Missouri_Rolla/Team">Team</a> | | <a href="https://2016.igem.org/Team:Missouri_Rolla/Team">Team</a> |
| | <a href="https://2016.igem.org/Team:Missouri_Rolla/Safety">Safety</a> | | <a href="https://2016.igem.org/Team:Missouri_Rolla/Safety">Safety</a> |
| − | <a href="https://2016.igem.org/Team:Missouri_Rolla">Attributions</a> | + | <a href="https://2016.igem.org/Team:Missouri_Rolla/Attributions">Attributions</a> |
| | </div> | | </div> |
| | + | <img id="bat2" src="https://static.igem.org/mediawiki/2016/7/71/T--Missouri_Rolla--joemicrobebat2.png"><img id="bat1" src="https://static.igem.org/mediawiki/2016/b/be/T--Missouri_Rolla--joemicrobebat1.png"> |
| | <div class="mstigem"> | | <div class="mstigem"> |
| − | <h1>Defending North American bats from the emerging White-Nose epidemic</h1> | + | <h1>Defending North American bats from the emerging White Nose epidemic</h1> |
| − | <hr>
| + | <p>Bats play a major role in the ecosystem and economy here in Missouri and across North America. According to the USGS, bats are the primary consumers of insects in temperate regions. The insect suppression service that the bats provide saves the nation’s farmers between four and fifty billion dollars a year in lost crops and pesticide costs. Bats then produce waste that becomes the primary input of nutrients to Missouri’s over 7000 caves and allows the caves to support diverse and unique life.</p> |
| − | <p>Here in Missouri and across the United States, bats play a major role in the ecosystem and economy. According to the USGS, bats are the primary consumers of insects in temperate regions. The insect suppression service that the bats provide saves the nation’s farmers between four and fifty billion dollars a year in lost crops and pesticide costs. Bats then produce waste that becomes the primary input of nutrients to Missouri’s over 7000 caves and allows the caves to support diverse and unique life.</p> | + | |
| | </div> | | </div> |
| | <img class="fullimg" src="https://static.igem.org/mediawiki/2016/b/ba/T--Missouri_Rolla--tricolorbatlucas.jpg"> | | <img class="fullimg" src="https://static.igem.org/mediawiki/2016/b/ba/T--Missouri_Rolla--tricolorbatlucas.jpg"> |
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| | <img class="fullimg" src="https://static.igem.org/mediawiki/2016/2/24/T--Missouri_Rolla--batwns.jpg"> | | <img class="fullimg" src="https://static.igem.org/mediawiki/2016/2/24/T--Missouri_Rolla--batwns.jpg"> |
| | <div class="mstigem"> | | <div class="mstigem"> |
| − | <p>[WHY NO ANTIFUNGALS, OCIMENE]</p> | + | <p>We investigated two approaches to defend bats from WNS while avoiding disturbing their hibernation, killing beneficial fungi, or releasing genetically-modified bacteria. We designed pathways on plasmids for the production of ocimene, a volatile organic compound, and leupeptin, a protease inhibitor, to reduce the severity of disease in different ways.</p> |
| − | <p>[LEUPEPTIN DESCRIPTION AND LINK TO PROJECT PAGE]</p>
| + | <p>Continue to our <a href="https://2016.igem.org/Team:Missouri_Rolla/Project">project page</a> to learn more!</p> |
| − | <div id="nb-accordion">
| + | <p id="credit">Tricolor bat photo credit: Lucas Harper<br>WNS bat photo credit: Jonathan Mays, Wildlife Biologist, Maine Department of Inland Fisheries and Wildlife</p> |
| − | <div class="within-accordion">
| + | |
| − | <h3>16 October 2016</h3>
| + | |
| − | <div><h4 class="h4left"><b>Kira Buckowing</b></h4>
| + | |
| − | <h4 class="h4right"><b>Start: 12:00pm</b></h4>
| + | |
| − | <h4><b>Chemical Transformations</b></h4>
| + | |
| − | <b>Purpose:</b> To check on the most recent work and get the wanted ocimene synthase in a chlor resistance backbone.
| + | |
| − | <h4><b>Protocol:</b></h4>
| + | |
| − | HiFi assemblies were set up as per the NEB protocol and incubated in ther thermocylcler for 15 minutes at 50 C.
| + | |
| − | <h5>10/16 HiFi 1</h5>
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>9 μL</td>
| + | |
| − | <td>10/14 D1</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>1 μL</td>
| + | |
| − | <td>10/14 D4</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10 μL</td>
| + | |
| − | <td>HiFi Master Mix</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <h5>10/16 HiFi 2</h5>
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td>9 μL</td>
| + | |
| − | <td>10/14 D2</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>1 μL</td>
| + | |
| − | <td>10/14 D4</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10 μL</td>
| + | |
| − | <td>HiFi Master Mix</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | | + | |
| − | 14 broth Cultures were set up from colony growth on the 10/15 chemical transformation plates.
| + | |
| − | | + | |
| − | Chemical transformations were performed using the chemically competent cells from NEB and adding either 2 μL or 6 μL of the appropriate HiFi assembly product, noted in the products section below.
| + | |
| − | <h4><b>Stop: 3:30pm</b></h4>
| + | |
| − | <h4><b>Products:</b></h4>
| + | |
| − |
| + | |
| − | <table>
| + | |
| − | <tbody>
| + | |
| − | <tr>
| + | |
| − | <td><b>Label</b></td>
| + | |
| − | <td><b>Source</b></td>
| + | |
| − | <td><b>Description</b></td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 CT1 100 μL</td>
| + | |
| − | <td>10/16 HiFi 1</td>
| + | |
| − | <td>Chemically transformed plasmid hopefully containing ocimene synthase in the pSB1C3 iGEM shipping backbone, 2 μL added</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 CT1 250 μL</td>
| + | |
| − | <td>10/16 HiFi 1</td>
| + | |
| − | <td>Chemically transformed plasmid hopefully containing ocimene synthase in the pSB1C3 iGEM shipping backbone, 2 μL added</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 CT2 100 μL</td>
| + | |
| − | <td>10/16 HiFi 2</td>
| + | |
| − | <td>Chemically transformed plasmid hopefully containing ocimene synthase in the pSB1C3 iGEM shipping backbone, 2 μL added</td>
| + | |
| − | </tr>
| + | |
| − | <td>10/16 CT2 250 μL</td>
| + | |
| − | <td>10/16 HiFi 2</td>
| + | |
| − | <td>Chemically transformed plasmid hopefully containing ocimene synthase in the pSB1C3 iGEM shipping backbone, 2 μL added</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 CT3 100 μL</td>
| + | |
| − | <td>10/16 HiFi 1</td>
| + | |
| − | <td>Chemically transformed plasmid hopefully containing ocimene synthase in the pSB1C3 iGEM shipping backbone, 6 μL added</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 CT3 250 μL</td>
| + | |
| − | <td>10/16 HiFi 1</td>
| + | |
| − | <td>Chemically transformed plasmid hopefully containing ocimene synthase in the pSB1C3 iGEM shipping backbone, 6 μL added</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 CT4 100 μL</td>
| + | |
| − | <td>10/16 HiFi 2</td>
| + | |
| − | <td>Chemically transformed plasmid hopefully containing ocimene synthase in the pSB1C3 iGEM shipping backbone, 6 μL added</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 CT4 250 μL</td>
| + | |
| − | <td>10/16 HiFi 2</td>
| + | |
| − | <td>Chemically transformed plasmid hopefully containing ocimene synthase in the pSB1C3 iGEM shipping backbone, 6 μL added</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 HiFi 1</td>
| + | |
| − | <td>10/14 D1 and D4</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr> | + | |
| − | <tr> | + | |
| − | <td>10/16 HiFi 2</td>
| + | |
| − | <td>10/14 D2 and D4</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC1</td>
| + | |
| − | <td>10/15 CT2 100 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC2</td>
| + | |
| − | <td>10/15 CT2 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC3</td>
| + | |
| − | <td>10/15 CT2 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC4</td>
| + | |
| − | <td>10/15 CT2 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC5</td>
| + | |
| − | <td>10/15 CT2 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC6</td>
| + | |
| − | <td>10/15 CT4 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC7</td>
| + | |
| − | <td>10/15 CT4 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC8</td>
| + | |
| − | <td>10/15 CT4 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC9</td>
| + | |
| − | <td>10/15 CT4 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC10</td>
| + | |
| − | <td>10/15 CT4 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC11</td>
| + | |
| − | <td>10/15 CT4 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC12</td>
| + | |
| − | <td>10/15 CT4 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC13</td>
| + | |
| − | <td>10/15 CT4 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td>10/16 BC14</td>
| + | |
| − | <td>10/15 CT4 250 μL</td>
| + | |
| − | <td>Hopefully the assembled product of ocimene in the iGEM shipping backbone</td>
| + | |
| − | </tr>
| + | |
| − | </tbody>
| + | |
| − | </table>
| + | |
| − | <h4><b>Next: Minipreps of any growth in the broth cultures and checking the plates to see if anything grows. </b></h4></div>
| + | |
| − | </div> | + | |
| − | <div class="within-accordion">
| + | |
| − | <h3>Accordion test 2</h3>
| + | |
| − | <div>Test 2</div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | </div> | | </div> |
| | <img id="flashlight" src="https://static.igem.org/mediawiki/2016/d/d4/T--Missouri_Rolla--flashlight.png"> | | <img id="flashlight" src="https://static.igem.org/mediawiki/2016/d/d4/T--Missouri_Rolla--flashlight.png"> |
Defending North American bats from the emerging White Nose epidemic
Bats play a major role in the ecosystem and economy here in Missouri and across North America. According to the USGS, bats are the primary consumers of insects in temperate regions. The insect suppression service that the bats provide saves the nation’s farmers between four and fifty billion dollars a year in lost crops and pesticide costs. Bats then produce waste that becomes the primary input of nutrients to Missouri’s over 7000 caves and allows the caves to support diverse and unique life.
Unfortunately, in 2007 a disease that is now called White Nose Syndrome (WNS) appeared in the bat populations of the northeastern United States. It is caused by a white fungus, Pseudogymnoascus destructans. The fungus grows on the bats while they hibernate and causes skin lesions leading to inflammation. This inflammation and irritation wakes the bats from their hibernation, causing them to burn precious fat reserves while it is still winter. The bats then starve or die from shock before spring arrives. The fungus has spread rapidly across the eastern United States and has left behind it high mortality. Generally, bat populations have declined by 80% with some populations totally lost.
We investigated two approaches to defend bats from WNS while avoiding disturbing their hibernation, killing beneficial fungi, or releasing genetically-modified bacteria. We designed pathways on plasmids for the production of ocimene, a volatile organic compound, and leupeptin, a protease inhibitor, to reduce the severity of disease in different ways.
Continue to our project page to learn more!
Tricolor bat photo credit: Lucas Harper
WNS bat photo credit: Jonathan Mays, Wildlife Biologist, Maine Department of Inland Fisheries and Wildlife
