Difference between revisions of "Team:Virginia"

 
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<!---------------------------Top image on page------------------------------------------>
 
<!---------------------------Top image on page------------------------------------------>
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<div class="homeimagearea">
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<h1 style="line-height:230px">LEU LOCK</h1>
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<!---------------------------Main content section of page------------------------------->
 
<!---------------------------Main content section of page------------------------------->
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<div class="content">
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<p><span class="p">
 
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<span class="ptitle">How did we choose our project?</span><br><br> Our team split into three small groups in March and each proposed a refined project idea.
 
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Our other ideas included lysin synthesis to treat acne and the development of a bamboo chassis to combat air pollution.
 
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After much heated debate and further research into project feasibility, we chose to focus our research on biocontainment.
 
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We wanted to use a large-scale approach to improve the field of synthetic biology.
 
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By developing a reliable biocontrol standard, we aim to promote practical, safe implementation of biological devices.
 
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<br><br><span class="ptitle">Project Description</span><br><br>
 
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The field of synthetic biology currently struggles with the issue of containment both in laboratory settings and real-world environments.
 
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This shortcoming prevents the widespread implementation of useful engineered devices and calls for a cellular-based containment system that can operate in an open environment and provide security comparable to physical containment.
 
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Although several biological methods currently exist for containment, these methods allow some degree of genetic escape through horizontal gene transfer, spontaneous mutagenesis, or utilization of environmentally
 
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available compounds.
 
  
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<span class="references"><sup>ref</sup><span class="refbox"><b>Reference:</b><br>Mandell, Daniel J., Marc J. Lajoie, Michael T. Mee, Ryo Takeuchi, Gleb Kuznetsov, Julie E. Norville, Christopher J. Gregg, Barry L. Stoddard, and George M. Church.
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<div class="content1">
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"Corrigendum: Biocontainment of Genetically Modified Organisms by Synthetic Protein Design." Nature 527.7577 (2015): 264. Web.
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<div id="introvideobkg">
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<a href="http://www.nature.com/nature/journal/v527/n7577/full/nature15536.html" target="_blank">(link)</a></span></span>  
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<div id="introvideo">
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<video id="vid1" width="569px" height="320px" controls preload="none">
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<source src="https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Biocontainmentvideo.mp4" type="video/mp4">
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</video>
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</div>
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<span id="vidcap"><i>Want to learn more about synthetic biology? Click <a href="https://2016.igem.org/Team:Virginia/Public_Education" style="color:#ffec7b">here</a>.</i></span>
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</div>
  
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The Virginia iGEM team proposes to use the CRISPR/Cas9 system to redesign leucyl-tRNA synthetase to confer metabolic dependence on modified leucine in <i>Escherichia coli</i>.
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Our goal is to create the foundation for a reliable, standardized, and universally applicable biocontainment system.
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</span></p>
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<div><h1>WHO ARE WE?</h1></div>
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<p><span class="p">We are a team of undergraduate students from the University of Virginia with a shared interest in synthetic biology.
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We hail from five different states, and our majors include biology, biochemistry, chemical engineering, biomedical engineering,
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neuroscience, statistics, and nanomedicine engineering.<br>
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</span></p>
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</div>
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<a href="https://2016.igem.org/Team:Virginia/Team">More about the team &rarr;</a>
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</div>
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</div>
  
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<div id="back1"></div><div id="back2"></div><div id="back3"></div><div id="back4"></div><div id="back5"></div>
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<div><h1>THE PROJECT</h1></div>
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<h1>Synthetic Translational Control</h1>
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<p><span class="p">We set out to design a novel biocontainment mechanism based upon the modification of the leucyl tRNA synthetase enzyme.
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Our system causes the biocontained organism to be dependent upon a synthetic form of leucine for survival -
 +
hence the title of our project. To implement the system in <i>E. coli</i>, our goal was used to knockout the native
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<i>E. coli</i> tRNA synthetase gene, and insert the modified synthetase gene into the bacterial cells.
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</span></p>
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</div>
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                    <a href="https://2016.igem.org/Team:Virginia/Description">Project Overview &rarr;</a>
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Latest revision as of 03:38, 20 October 2016

Want to learn more about synthetic biology? Click here.

WHO ARE WE?

We are a team of undergraduate students from the University of Virginia with a shared interest in synthetic biology. We hail from five different states, and our majors include biology, biochemistry, chemical engineering, biomedical engineering, neuroscience, statistics, and nanomedicine engineering.

More about the team →

THE PROJECT

Synthetic Translational Control

We set out to design a novel biocontainment mechanism based upon the modification of the leucyl tRNA synthetase enzyme. Our system causes the biocontained organism to be dependent upon a synthetic form of leucine for survival - hence the title of our project. To implement the system in E. coli, our goal was used to knockout the native E. coli tRNA synthetase gene, and insert the modified synthetase gene into the bacterial cells.

Project Overview →