Difference between revisions of "Team:BIT/Demonstrate"

 
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     <li><a href="https://2016.igem.org/Team:BIT/Description"><h2>Description</h2></a></li>
 
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<h2><br><br><br><br><br><br><br>Model</h2>
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<h2><br><br><br><br><br><br><br>Demonstrate</h2>
 
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<p>The following results show our project working under real-world conditions. We employed three ways to demonstrate our project working under simulated conditions in the lab including gel electrophoresis, sequencing and experiment test.</p>
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Chinese Premier Li Keqiang delivers a keynote speech on October 11 at the opening ceremony of the Fifth Ministerial Conference of The Forum for Economic and Trade Cooperation Between China and Portuguese-speaking Countries in Macao. [Photo/Xinhua]<br><br></p>
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<div style="text-indent: 0em;">
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<p>
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<p><b>1. Live cell Logic gate genetic components</b></p>
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Premier Li Keqiang announced 18 measures on Tuesday in Macao to boost ties with Portuguese-speaking countries in fields such as production capacity, people-to-people exchanges and maritime development.<br><br>
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</div>
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</p>
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<img src="https://static.igem.org/mediawiki/2016/f/f6/2016.igem.orgwikiimages16BIT.png" class="img-responsive" alt="..."><br>
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<p>
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<p>According to this picture, the NO.1 and No. 2 are J23104+B0034+Mir155 Binding site+Gfp (about 1200bp)</p>
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The measures will be implemented in the next three years, and the Chinese government will support the Macao Special Administrative Region in establishing a financial service program, cultural exchange centers, bilingual training bases and juvenile innovation centers for exchanges with these countries, the premier said.<br><br>
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<p>J23104(35bp)+B0034(12bp)+Mir155(97bp)+Gfp(878bp)+B0015(129bp)=1151(bp)</p>
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</p>
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<p>J23104(35bp) +B0034(12bp)+Mir21(90)+Rfp(706bp)+B0015(129bp)=972(bp)</p>
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<p>
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<p>So, the picture proves that that the length of our device is right. No.3 gene located on 30000bp is target gene (972bp) and backbone(2000bp).</p>
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China resumed sovereignty over Macao in December 1999 after it had been ruled since the mid-16th century by Portugal.<br><br>
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<br>
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</p>
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<br>
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<p>
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<img src="https://static.igem.org/mediawiki/2016/e/e8/2016.igem.orgwikiimages17BIT.png" class="img-responsive" alt="...">
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Beijing is willing to carry out major projects with these countries by making use of the China-Portuguese-Speaking Countries Cooperation Development Fund and the Silk Road Fund, Li said.<br><br>
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<br>
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</p>
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<p>Ptet(54bp)+B0034(12bp)+tetR(685bp)+B0034(129bp)+FimE(558bp)+B0015(129bp)+pLac(35bp)+B0034(129bp)+LacI(1125bp)+<br>B0034(12bp)+BXB1(1503bp)+B0015(129bp)+IRL+B0010+IRR+attB+B0010+attP(300bp)+B0034(12bp)+Gfp(878bp)+B0015(129bp)=5809(bp)</p>
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<p>
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<p>So, the picture prove that that the length of our device is right.(No.2,No.4,No.6)</p>
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The announcement was made in Li's keynote speech on Tuesday at the opening ceremony of the two-day Fifth Ministerial Conference of the Forum for Economic and Trade Cooperation Between China and Portuguese-Speaking Countries.<br><br>
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</p>
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<br>
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<p>
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<img src="https://static.igem.org/mediawiki/2016/7/78/2016.igem.orgwikiimages18BIT.png" class="img-responsive" alt="..."><br>
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Delegates from seven Portuguese speaking countries — Portugal, Brazil, Cape Verde, Guinea-Bissau, Mozambique, Angola and East Timor — attended the forum.<br><br>
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<p>Pconst(55bp)+B0034(12bp)+Mir21(90bp)+LacI(1125bp)+B0015(129)+Plac+key(plac and key 90bp)+Plac+Lock(Plac and Lock 126bp)+Gfp(878bp)+B0015(129bp)=1756(bp)</p>
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</p>
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<p>So, the picture prove that that the length of our device is right.(No.2,No.4,No.6)</p>
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<p>
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<br>
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Before the ceremony, Li launched a trade and economic cooperation program to promote further exchanges between China and Portuguese-speaking countries.
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<br>
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<br><br>
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<img src="https://static.igem.org/mediawiki/2016/d/d0/2016.igem.orgwikiimages19BIT.png" class="img-responsive" alt="..."><br>
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</p>
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<p>Pconst(55bp)+B0034(12bp)+Mir21(90bp)+LacI(1250bp)+B0015(129)+Plac+key(plac and key 90)+Plac+Lock(Plac and Lock 126bp)+BXB1(1503) +B0015(129bp)=3384(bp)</p>
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<p>
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<p>So, the picture prove that that the length of our device is right.(No.2,NO.4)</p>
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China and the Portuguese-speaking countries contribute 17 percent of the world economy and 22 percent of global population, with varying advantages in technology, capital and market practices, Li said.<br><br>
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</p>
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<p>
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<br>
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The participating countries should consolidate political trust, promote trade liberalization and investment and strengthen cooperation in production capacity and people-to-people exchanges, the premier told the forum.<br><br>
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<div style="text-indent: 0em;">
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</p>
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<p><b>2. Sequencing</b></p>
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<img src="https://static.igem.org/mediawiki/2016/1/1a/BIT_test.jpg" class="img-responsive" alt="...">
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</div>
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<br>
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<p>We sequencing and alignment J23104-B0034-mi155-4-E0040-B0010-B0012</p>
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<p>
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<img src="https://static.igem.org/mediawiki/2016/b/b5/2016.igem.orgwikiimages20BIT.png" class="img-responsive" alt="..."><br>
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China's economic restructuring and upgrading will bring great investment opportunities and market potential, creating bright prospects for businesses from Portuguese-speaking countries, Li said.<br><br>
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<p>The identities are 99% right because of gene sequencing error and gene mutation. So this picture prove our device is right.</p>
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</p>
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<br><br><br>
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<p>
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<div style="text-indent: 0em;">
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He said the trade volume between China and Portuguese-speaking countries reached $100 billion last year — about 10 times of that of 2003.<br><br>
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<p><b>3. Experiment test</b></p>
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</p>
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</div>
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<p>
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<br>
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Cape Verde's Prime Minister Ulisses Correia e Silva said the Macao forum injected new momentum into strategic cooperation between all the participating countries. He added that his country will align with China's Belt and Road Initiative and make use of the Macao forum to bring more commodities to Africa.<br><br>
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<img src="https://static.igem.org/mediawiki/2016/2/25/2016.igem.orgwikiimages21BIT.png" class="img-responsive" alt="..."><br>
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</p>
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<p>This is the growth curve of bacteria and from this figure, we can see that as time goes on, the bacteria reproduce which results in the rising concentration and OD value of bacterium. As for different experimental groups, all the groups with different concentration of mir155 have almost the same OD value, which indicate the growth of bacteria are synchronous during the detection of florescence. The factor of concentration of mir155 does not have strong impact on the growth of bacteria which accords with expectations of experimental results.</p>
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<p>
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<br>
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The prime ministers of Guinea-Bissau and Mozambique vowed to adopt favorable policies and improve the rule of law to attract investors.<br><br>
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<img src="https://static.igem.org/mediawiki/2016/b/b5/2016.igem.orgwikiimages22BIT.png" class="img-responsive" alt="..."><br>
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</p>
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<p>This figure show the fluorescence value generated by per unit bacteria volume which is also the experimental result of Part BBa_K2041021. When the mir155 presents in the system, mir155 will combine with the binding site. Meanwhile, the combination will influence the transcription and translation of downriver gene. Therefore, the expression of fluorescence will decrease and with the higher concentration of mir155, the expression of GFP will bring down gradually. After culturing for a while, fluorescence value generated by per unit bacteria volume will tend to a stable value.</p>
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<p>
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<br>
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Deng Yanzi contributed to this story<br><br><br><br><br><br>
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<img src="https://static.igem.org/mediawiki/2016/a/a2/2016.igem.orgwikiimages23BIT.png" class="img-responsive" alt="..."><br>
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</p>
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<p>This is the growth curve of bacteria and from this figure, we can see that as time goes on, the bacteria reproduce which results in the rising concentration and OD value of bacterium. As for different experimental groups, all the groups with different concentration of tetR have almost the same OD value, which indicate the growth of bacteria are synchronous during the detection of florescence. The factor of concentration of tetR does not have strong impact on the growth of bacteria which accords with expectations of experimental results.</p>
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<p>For more details: https://2016.igem.org/Team:BIT/Biology</p>
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Latest revision as of 03:41, 20 October 2016

<!DOCTYPE html> Biology

ACHIEVEMENT

DEMONSTRATE








Demonstrate

The following results show our project working under real-world conditions. We employed three ways to demonstrate our project working under simulated conditions in the lab including gel electrophoresis, sequencing and experiment test.


1. Live cell Logic gate genetic components

...

According to this picture, the NO.1 and No. 2 are J23104+B0034+Mir155 Binding site+Gfp (about 1200bp)

J23104(35bp)+B0034(12bp)+Mir155(97bp)+Gfp(878bp)+B0015(129bp)=1151(bp)

J23104(35bp) +B0034(12bp)+Mir21(90)+Rfp(706bp)+B0015(129bp)=972(bp)

So, the picture proves that that the length of our device is right. No.3 gene located on 30000bp is target gene (972bp) and backbone(2000bp).



...

Ptet(54bp)+B0034(12bp)+tetR(685bp)+B0034(129bp)+FimE(558bp)+B0015(129bp)+pLac(35bp)+B0034(129bp)+LacI(1125bp)+
B0034(12bp)+BXB1(1503bp)+B0015(129bp)+IRL+B0010+IRR+attB+B0010+attP(300bp)+B0034(12bp)+Gfp(878bp)+B0015(129bp)=5809(bp)

So, the picture prove that that the length of our device is right.(No.2,No.4,No.6)



...

Pconst(55bp)+B0034(12bp)+Mir21(90bp)+LacI(1125bp)+B0015(129)+Plac+key(plac and key 90bp)+Plac+Lock(Plac and Lock 126bp)+Gfp(878bp)+B0015(129bp)=1756(bp)

So, the picture prove that that the length of our device is right.(No.2,No.4,No.6)



...

Pconst(55bp)+B0034(12bp)+Mir21(90bp)+LacI(1250bp)+B0015(129)+Plac+key(plac and key 90)+Plac+Lock(Plac and Lock 126bp)+BXB1(1503) +B0015(129bp)=3384(bp)

So, the picture prove that that the length of our device is right.(No.2,NO.4)




2. Sequencing

We sequencing and alignment J23104-B0034-mi155-4-E0040-B0010-B0012

...

The identities are 99% right because of gene sequencing error and gene mutation. So this picture prove our device is right.




3. Experiment test


...

This is the growth curve of bacteria and from this figure, we can see that as time goes on, the bacteria reproduce which results in the rising concentration and OD value of bacterium. As for different experimental groups, all the groups with different concentration of mir155 have almost the same OD value, which indicate the growth of bacteria are synchronous during the detection of florescence. The factor of concentration of mir155 does not have strong impact on the growth of bacteria which accords with expectations of experimental results.


...

This figure show the fluorescence value generated by per unit bacteria volume which is also the experimental result of Part BBa_K2041021. When the mir155 presents in the system, mir155 will combine with the binding site. Meanwhile, the combination will influence the transcription and translation of downriver gene. Therefore, the expression of fluorescence will decrease and with the higher concentration of mir155, the expression of GFP will bring down gradually. After culturing for a while, fluorescence value generated by per unit bacteria volume will tend to a stable value.


...

This is the growth curve of bacteria and from this figure, we can see that as time goes on, the bacteria reproduce which results in the rising concentration and OD value of bacterium. As for different experimental groups, all the groups with different concentration of tetR have almost the same OD value, which indicate the growth of bacteria are synchronous during the detection of florescence. The factor of concentration of tetR does not have strong impact on the growth of bacteria which accords with expectations of experimental results.

For more details: https://2016.igem.org/Team:BIT/Biology






Contact Us

Address

Beijing Institute of Technology,
No. 5 South Zhong Guan Cun Street,
Haidian Beijing 100081, P. R. China

Twitter : @igem_BIT

Sina Weibo : @igem_BIT

Website : http://www.bit.edu.cn
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