Difference between revisions of "Team:Alverno CA/Notebook"

Latest revision as of 03:49, 20 October 2016

Alverno iGEM 2016

Notebook

Week of June 6th (Limited amount of notes taken)

- Lots of Designing Parts and Primers

- Wiki Work

Week of June 13th:

- Cleaning Restriction Digest and Ligation Done

- Ampicillin plates made

- Ampicillin stock made

- Transform RFP plasmid

- Design Parts and Primers

- Wiki Work

Week of June 20th

- Transformation Done (probably GFP)

- RFP and GRP colonies

- PCR for RFP Coding Gene

- Design Parts and Primers

- Wiki Work

Week of June 27th

- PCR Done for promoters, terminators, some UC parts

- Primer Dilutions Done

- Gel Run with PCR product from Week of June 20th (RFP)

- Miniprep kit for RFP Bba

- TBE Buffer 10x and 1x made

- PCR Purification done on Promoters and Terminators

- Wiki Work

Week of July 4th

- PCR of Vectors, some UC parts

- Primer Dilutions

- Gel Run UC16-18 and UC2 (UC18 ran twice)

- PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)

- Plate Reader

- Wiki Work

Week of July 11th

- Learn how to use Benchling

- Primers Diluted

- Parts Diluted

- PCR done on P1ab, P2ab, T3ab, T4ab

- Gel Run on P1ab, P2ab, T3ab, T4ab

- PCR Clean up done on P1ab, P2ab, T3ab, T4ab

- Chloramphenicol plates made

- Wiki Work

Week of July 18th

- UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)

- Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d

- Transformation Done

- Chloramphenicol plates made

- Parts dilutions Done

- PCR of GG Assemblies

- PCR of UC3a-UC9a

- Wiki Work

Week of July 25th

- Set up Plate reader

- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts

- PCR Purification of UC3-9, V1a & V2a

- Picked Colonies

- Transformation Protocol for GG1-8

- Gel made & Gel made w/ SYBR safe

- PCR of GG Assemblies

- PCR for V1a and V2a, UC7-9

- Dilutions Done

- Gel Run for UC7-9

- Golden Gate Assembly GG9-16

- Wiki Work

Week of August 1st

- Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23

- PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12

- PCR Purification for UC7-8

- Parts Dilutions done UC2 & UC3-6 & UC7-8

- PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23

- Fixed Thermocycler

- Gels Made

- Agar Plates made w/ Kan

- Golden Gate Assembly GG17-19 & GG20-23 & GG24-26

- Transformation with GG12

- Ludox Calibration

- Test Fluorescence of Plasmids

- Wiki Work

Week of August 8th

- Cell Measurement

- Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR

- PCR Check for GG24-26 & GG27-28

- Made Gel

- Transformation with GG24-26 & GG27-28

- Golden Gate Assembly GG27-28

- Colony PCR Check of GG24-26

- Primer Dilutions

- Plasmid DNA Purification

- LB Kan Broth

- Wiki Work

Week of August 15th

- Lab Meet-up with UCL/LC/CV

- Colony PCR Check for GG libraries 27-28

- Gels Made

- Gel Run for GG Library 28

- Grow Cultures GG27

- Learn Python

- Check Fluorescence for GG28 & GG27

- PCR UC14a

- Purification of Plasmid DNA

- Wiki Work

Week of August 22nd

- Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36

- Gels Made

- PCR UC13a and UC15a (Done twice) & UC1

- PCR Purification of UC14 & UC13/15 & UC1

- Golden Gate Assembly for GG29-36

- PCR Check for GG29-36

- LB Kan Broth Made

- Dilution of UC1

- Control run in Plate Reader

- Wiki Work

Week of August 29th

- Make Gels

- Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a

- Golden Gate Assembly GG37-52 & GG53-60

- PCR Check GG37-52 & GG53-60

- PCR Check Gel run for GG37-52

- PCR CDS1a & CDS2a

- PCR Purification of CDS1a & CDS2a

- Talk with iGEM Peshawar

- LB Kan Plates Made

- Wiki Work

Week of September 5th

- iGEM Team Meeting

- PCR Purification of CDS1a & CDS2a and UC1

- Transformation was done with GG53-60

- Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64

- Gels made

- Dilution of CDS1a & CDS2a

- Golden Gate Assembly GG61-64

- PCR of UC1

- LB Kan Plates Made

- PCR Check for GG61-64

- TBE 10x Buffer

- Wiki Work

Week of September 12th

- Talked with UCL iGEM Team

- LV-LC-CV meet-up

- Google Hangouts with Lambert Georgia iGEM team

- Dilution of UC1a

- Transformation of GG29-36

- Golden Gate Assembly GG65-72

- PCR GG61-64

- PCR Check GG61-64

- TX-TL of GFP (+ control)

- Outreach workshop with Middle school

- Wiki Work

Week of September 19th

- REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE

- Run Gel for GG61-64 (done twice) and GG65-72

- LB Kan Plates Made

- Gels Made

- TX-TL GFP Re-run

- Gel Run for UC1 and GG61-64

- Control Test ran using only the ladder for Gels

- TX-TL W & M plasmids

- PCR U3a

- Dilution of Primers

- Wiki Work

Week of September 26th

- PCR Purification of V3a

- Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94

- PCR Check GG73-74 and GG75-82

- Dilution V3a

- Run Gel V3a

- Transformation of GG61-64 (done twice) and GG65-72 and GG73-74

- TX-TL re-run for W & M plasmids

- Colony PCR GG61-64 and GG65-72

- LB Chloramphenicol plates & LB Kanamycin plates

- Wiki Work

Week of October 3rd

- Middle School Visit

- LB Broth made

- PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab

- Dilution V3a

- PCR Check GG83-90

- PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab

- TX-TL re-run for W & M plasmids w/ Replicates

- Gels Made

- Mini prep GG28 1-5

- Make TBE 1x

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+<body>
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+<br>
+<br>
+<h2><center>Notebook</center></h2>
-     <div id="top-section">
+<center>
-	 <div id="p-logo">
+<img class="icons"src="https://static.igem.org/mediawiki/2016/a/ac/T--Alverno_CA--labnotbook.jpg" style= width:500px>
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+<h3>Week of June 6th (Limited amount of notes taken)</h3>
+<h5>- Lots of Designing Parts and Primers</h5>
+<h5>- Wiki Work</h5>
+<h3>Week of June 13th:</h3>
+<h5>- Cleaning Restriction Digest and Ligation Done</h5>
+<h5>- Ampicillin plates made</h5>
+<h5>- Ampicillin stock made</h5>
+<h5>- Transform RFP plasmid</h5>
+<h5>- Design Parts and Primers</h5>
+<h5>- Wiki Work</h5>
- 	<div id="menubar" class='left-menu noprint'>
+<h3>Week of June 20th</h3>
- 	  <ul>
+<h5>- Transformation Done (probably GFP)</h5>
- 	                   <li
+<h5>- RFP and GRP colonies</h5>
-                class='selected' 	       ><a href="/Team:Tec-Monterrey/ITESM14_project.html">Page               </a></li>
+<h5>- PCR for RFP Coding Gene</h5>
-               <li
+<h5>- Design Parts and Primers</h5>
-                class='new' 	       ><a href="/wiki/index.php?title=Talk:Team:Tec-Monterrey/ITESM14_project.html&amp;action=edit&amp;redlink=1">Discussion               </a></li>
+<h5>- Wiki Work</h5>
-               <li
-               	       ><a href="/wiki/index.php?title=Team:Tec-Monterrey/ITESM14_project.html&amp;action=edit">View source               </a></li>
-               <li
-               	       ><a href="/wiki/index.php?title=Team:Tec-Monterrey/ITESM14_project.html&amp;action=history">History               </a></li>
-              <li style='color:white;cursor:default'>teams</li>
- 	  </ul>
- 	</div> <!-- end menubar (left) -->
-	<div class="right-menu noprint" id="menubar">
+<h3>Week of June 27th</h3>
-	    <ul>
+<h5>- PCR Done for promoters, terminators, some UC parts</h5>
-                				<li id="pt-login"><a href="/wiki/index.php?title=Special:UserLogin&amp;returnto=Team:Tec-Monterrey/ITESM14_project.html" title="You are encouraged to log in; however, it is not mandatory [o]" accesskey="o">Log in</a></li> 	    </ul>
+<h5>- Primer Dilutions Done</h5>
-	</div><!-- end right menubar -->
+<h5>- Gel Run with PCR product from Week of June 20th (RFP)</h5>
+<h5>- Miniprep kit for RFP Bba</h5>
+<h5>- TBE Buffer 10x and 1x made</h5>
+<h5>- PCR Purification done on Promoters and Terminators</h5>
+<h5>- Wiki Work</h5>
-	<div id="search-controls" class="noprint">
+<h3>Week of July 4th</h3>
-		<form action="/Special:Search" id="searchform">
+<h5>- PCR of Vectors, some UC parts</h5>
-		<input id="searchInput" name="search" type="text" title="Search 2014.igem.org [f]" accesskey="f" value="" />
+<h5>- Primer Dilutions</h5>
-			 <input type='submit' name="go" class="searchButton" id="searchGoButton"	value="Go" title="Go to a page with this exact name if exists" />&nbsp;
+<h5>- Gel Run UC16-18 and UC2 (UC18 ran twice)</h5>
-		       <input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
+<h5>- PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)</h5>
-		</form>
+<h5>- Plate Reader</h5>
-	</div> <!-- close search controls -->
+<h5>- Wiki Work</h5>
-    </div> <!-- close top-section-->
-    <div id="content">
-		<a name="top" id="top"></a>
-				<h1 class="firstHeading">Team:Tec-Monterrey/ITESM14 project.html</h1>
-		<div id="bodyContent">
-			<h3 id="siteSub" class='noprint'>From 2014.igem.org</h3>
-			<div id="contentSub"></div>
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-  <meta http-equiv="X-UA-Compatible" content="IE=edge">
-  <meta name="viewport" content="width=device-width, initial-scale=1">
-  <title>Project</title>
+<h3>Week of July 11th</h3>
-  <link rel="shortcut icon" href="images/favicon.ico"/>
+<img src="https://static.igem.org/mediawiki/2016/4/44/T--Alverno_CA--Screenshot_2016-10-15_09.24.58.png" alt="iGEM" style="max-width:100%; max-height:100%">
+<h5>- Learn how to use Benchling</h5>
+<h5>- Primers Diluted</h5>
+<img src="https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.05.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Parts Diluted</h5>
+<h5>- PCR done on P1ab, P2ab, T3ab, T4ab</h5>
+<h5>- Gel Run on P1ab, P2ab, T3ab, T4ab</h5>
+<h5>- PCR Clean up done on P1ab, P2ab, T3ab, T4ab</h5>
+<h5>- Chloramphenicol plates made</h5>
+<h5>- Wiki Work</h5>
-  <!-- Javascript -->
+<h3>Week of July 18th</h3>
-  <script src="https://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script>
+<h5>- UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)</h5>
-  <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/js/bootstrap.min.js"></script>
+<img src="https://static.igem.org/mediawiki/2016/d/d5/T--Alverno_CA--Screenshot_2016-10-15_09.05.53.png" alt="iGEM" style="width:30%; height:30%">
-  <script src="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_lightbox.min.js?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
+<h5>- Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d</h5>
-  <script src="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_main.js?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
+<img src="https://static.igem.org/mediawiki/2016/5/5d/T--Alverno_CA--Screenshot_2016-10-15_09.06.03.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Transformation Done</h5>
+<h5>- Chloramphenicol plates made</h5>
+<h5>- Parts dilutions Done</h5>
+<h5>- PCR of GG Assemblies</h5>
+<h5>- PCR of UC3a-UC9a</h5>
+<h5>- Wiki Work</h5>
-  <!--CSS Reset -->
+<h3>Week of July 25th</h3>
-  <link href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_reset.css?action=raw&amp;ctype=text/css" type="text/css" rel="stylesheet">
+<img src="https://static.igem.org/mediawiki/2016/4/4f/T--Alverno_CA--Screenshot_2016-10-15_09.06.12.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Set up Plate reader</h5>
+<h5>- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts</h5>
+<h5>- PCR Purification of UC3-9, V1a & V2a</h5>
+<img src="https://static.igem.org/mediawiki/2016/d/d9/T--Alverno_CA--Screenshot_2016-10-15_09.06.21.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Picked Colonies</h5>
+<h5>- Transformation Protocol for GG1-8</h5>
+<h5>- Gel made & Gel made w/ SYBR safe</h5>
+<h5>- PCR of GG Assemblies</h5>
+<h5>- PCR for V1a and V2a, UC7-9</h5>
+<h5>- Dilutions Done</h5>
+<h5>- Gel Run for UC7-9</h5>
+<h5>- Golden Gate Assembly GG9-16</h5>
+<h5>- Wiki Work</h5>
-  <!-- Bootstrap CSS -->
+<h3>Week of August 1st</h3>
-  <link href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css?action=raw&amp;ctype=text/css" type="text/css" rel="stylesheet">
+<h5>- Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23</h5>
-  <link href="https://maxcdn.bootstrapcdn.com/font-awesome/4.2.0/css/font-awesome.min.css" rel="stylesheet">
+<h5>- PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12</h5>
+<h5>- PCR Purification for UC7-8</h5>
+<h5>- Parts Dilutions done UC2 & UC3-6 & UC7-8</h5>
+<h5>- PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23</h5>
+<img src="https://static.igem.org/mediawiki/2016/d/d6/T--Alverno_CA--Screenshot_2016-10-15_09.06.31.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Fixed  Thermocycler</h5>
+<h5>- Gels Made</h5>
+<h5>- Agar Plates made w/ Kan</h5>
+<h5>- Golden Gate Assembly GG17-19 & GG20-23 & GG24-26</h5>
+<img src="https://static.igem.org/mediawiki/2016/c/c2/T--Alverno_CA--Screenshot_2016-10-15_09.06.45.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Transformation with GG12</h5>
+<h5>- Ludox Calibration</h5>
+<h5>- Test Fluorescence of Plasmids</h5>
+<h5>- Wiki Work</h5>
-  <!--Custom CSS -->
+<h3>Week of August 8th</h3>
-  <link href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_main.css?action=raw&amp;ctype=text/css" type="text/css" rel="stylesheet">
+<img src="https://static.igem.org/mediawiki/2016/5/53/T--Alverno_CA--Screenshot_2016-10-15_09.06.52.png" alt="iGEM" style="width:20%; height:20%">
-  <link href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_navbar.css?action=raw&amp;ctype=text/css" type="text/css" rel="stylesheet">
+<h5>- Cell Measurement</h5>
-  <link href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_lightbox.css?action=raw&amp;ctype=text/css" type="text/css" rel="stylesheet">
+<img src="https://static.igem.org/mediawiki/2016/0/0e/T--Alverno_CA--Screenshot_2016-10-15_09.07.03.png" alt="iGEM" style="width:20%; height:20%">
-  <link href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_tab.css?action=raw&amp;ctype=text/css" type="text/css" rel="stylesheet">
+<h5>- Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR</h5>
-  <link href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_team.css?action=raw&amp;ctype=text/css" type="text/css" rel="stylesheet">
+<h5>- PCR Check for GG24-26 & GG27-28</h5>
+<h5>- Made Gel</h5>
+<h5>- Transformation with GG24-26 & GG27-28</h5>
+<h5>- Golden Gate Assembly GG27-28</h5>
+<h5>- Colony PCR Check of GG24-26</h5>
+<h5>- Primer Dilutions</h5>
+<h5>- Plasmid DNA Purification</h5>
+<h5>- LB Kan Broth</h5>
+<h5>- Wiki Work</h5>
-  <link href='http://fonts.googleapis.com/css?family=Yanone+Kaffeesatz:400,200,300,700&amp;subset=latin,latin-ext' rel='stylesheet' type='text/css'>
+<h3>Week of August 15th</h3>
+<img src="https://static.igem.org/mediawiki/2016/5/5c/T--Alverno_CA--Screenshot_2016-10-15_09.07.17.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Lab Meet-up with UCL/LC/CV</h5>
+<h5>- Colony PCR Check for GG libraries 27-28</h5>
+<h5>- Gels Made</h5>
+<h5>- Gel Run for GG Library 28</h5>
+<h5>- Grow Cultures GG27</h5>
+<img src="https://static.igem.org/mediawiki/2016/0/0c/T--Alverno_CA--Screenshot_2016-10-15_09.07.26.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Learn Python</h5>
+<h5>- Check Fluorescence for GG28 & GG27</h5>
+<h5>- PCR UC14a</h5>
+<h5>- Purification of Plasmid DNA</h5>
+<h5>- Wiki Work</h5>
-  <script type="text/javascript" src="http://d3js.org/d3.v3.min.js"></script>
+<h3>Week of August 22nd</h3>
-  <script type="text/javascript" src="http://cdn.jsdelivr.net/cal-heatmap/3.3.10/cal-heatmap.min.js"></script>
+<img src="https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.07.34.png" alt="iGEM" style="width:20%; height:20%">
-  <link rel="stylesheet" href="http://cdn.jsdelivr.net/cal-heatmap/3.3.10/cal-heatmap.css" />
+<h5>- Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36</h5>
+<h5>- Gels Made</h5>
+<h5>- PCR UC13a and UC15a (Done twice) & UC1</h5>
+<h5>- PCR Purification of UC14 & UC13/15 & UC1</h5>
+<h5>- Golden Gate Assembly for GG29-36</h5>
+<h5>- PCR Check for GG29-36</h5>
+<h5>- LB Kan Broth Made</h5>
+<h5>- Dilution of UC1</h5>
+<h5>- Control run in Plate Reader</h5>
+<img src="https://static.igem.org/mediawiki/2016/6/69/T--Alverno_CA--Screenshot_2016-10-15_09.07.46.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Wiki Work</h5>
-  <link href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_katex.css?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css">
+<h3>Week of August 29th</h3>
-  <script src="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_katex.js?action=raw&amp;ctype=text/javascript" type="text/javascript"></script>
+<img src="https://static.igem.org/mediawiki/2016/9/9b/T--Alverno_CA--Screenshot_2016-10-15_09.07.54.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Make Gels</h5>
+<h5>- Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a</h5>
+<h5>- Golden Gate Assembly GG37-52 & GG53-60</h5>
+<h5>- PCR Check GG37-52 & GG53-60</h5>
+<h5>- PCR Check Gel run for GG37-52</h5>
+<h5>- PCR CDS1a & CDS2a</h5>
+<h5>- PCR Purification of CDS1a & CDS2a</h5>
+<img src="https://static.igem.org/mediawiki/2016/d/d7/T--Alverno_CA--Screenshot_2016-10-15_09.08.02.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Talk with iGEM Peshawar</h5>
+<h5>- LB Kan Plates Made</h5>
+<h5>- Wiki Work</h5>
-  <script>
+<h3>Week of September 5th</h3>
-    $(function(){
+<img src="https://static.igem.org/mediawiki/2016/a/ae/T--Alverno_CA--Screenshot_2016-10-15_09.08.11.png" alt="iGEM" style="width:20%; height:20%">
-      $("#wiki-header").load("https://2014.igem.org/Team:Tec-Monterrey/ITESM14_header.html?action=raw&amp;ctype=text/html");
+<h5>- iGEM Team Meeting</h5>
-    });
+<h5>- PCR Purification of CDS1a & CDS2a and UC1</h5>
-  </script>
+<h5>- Transformation was done with GG53-60</h5>
-  <script>
+<h5>- Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64</h5>
-    $(function(){
+<h5>- Gels made</h5>
-      $("#wiki-footer").load("https://2014.igem.org/Team:Tec-Monterrey/ITESM14_footer.html?action=raw&amp;ctype=text/html");
+<h5>- Dilution of CDS1a & CDS2a</h5>
-    });
+<h5>- Golden Gate Assembly GG61-64</h5>
-  </script>
+<h5>- PCR of UC1</h5>
-</head>
+<h5>- LB Kan Plates Made</h5>
+<h5>- PCR Check for GG61-64</h5>
+<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.20.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- TBE 10x Buffer</h5>
+<h5>- Wiki Work</h5>
-</p><p>
+<h3>Week of September 12th</h3>
+<img src="https://static.igem.org/mediawiki/2016/f/f2/T--Alverno_CA--Screenshot_2016-10-15_09.08.27.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Talked with UCL iGEM Team</h5>
+<h5>- LV-LC-CV meet-up</h5>
+<h5>- Google Hangouts with Lambert Georgia iGEM team</h5>
+<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.35.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Dilution of UC1a</h5>
+<h5>- Transformation of GG29-36</h5>
+<h5>- Golden Gate Assembly GG65-72</h5>
+<h5>- PCR GG61-64</h5>
+<h5>- PCR Check GG61-64</h5>
+<h5>- TX-TL of GFP (+ control)</h5>
+<img src="https://static.igem.org/mediawiki/2016/0/04/T--Alverno_CA--Screenshot_2016-10-15_09.08.42.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Outreach workshop with Middle school</h5>
+<h5>- Wiki Work</h5>
-  <script>
+<h3>Week of September 19th</h3>
-    $(function(){
+<h5>- REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE</h5>
-      $("#wiki-module1").load("https://2014.igem.org/Team:Tec-Monterrey/ITESM14_module1.html?action=raw&amp;ctype=text/html");
+<img src="https://static.igem.org/mediawiki/2016/4/4a/T--Alverno_CA--Screenshot_2016-10-15_09.08.50.png" alt="iGEM" style="width:20%; height:20%">
-    });
+<h5>- Run Gel for GG61-64 (done twice) and GG65-72</h5>
-  </script>
+<h5>- LB Kan Plates Made</h5>
-  <script>
+<h5>- Gels Made</h5>
-    $(function(){
+<h5>- TX-TL GFP Re-run</h5>
-      $("#wiki-module2").load("https://2014.igem.org/Team:Tec-Monterrey/ITESM14_module2.html?action=raw&amp;ctype=text/html");
+<h5>- Gel Run for UC1 and GG61-64</h5>
-    });
+<h5>- Control Test ran using only the ladder for Gels</h5>
-  </script>
+<h5>- TX-TL W & M plasmids</h5>
-  <script>
+<h5>- PCR U3a</h5>
-    $(function(){
+<h5>- Dilution of Primers</h5>
-      $("#wiki-module3").load("https://2014.igem.org/Team:Tec-Monterrey/ITESM14_module3.html?action=raw&amp;ctype=text/html");
+<img src="https://static.igem.org/mediawiki/2016/3/3f/T--Alverno_CA--Screenshot_2016-10-15_09.09.01.png" alt="iGEM" style="width:20%; height:20%">
-    });
+<h5>- Wiki Work</h5>
-  </script>
-  <script>
-    $(function(){
-      $("#wiki-module4").load("https://2014.igem.org/Team:Tec-Monterrey/ITESM14_module4.html?action=raw&amp;ctype=text/html");
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-  </script>
-  <script>
-    $(function(){
-      $("#wiki-modeling").load("https://2014.igem.org/Team:Tec-Monterrey/ITESM14_modeling.html?action=raw&amp;ctype=text/html");
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-  <script>
-    $(function(){
-      $("#wiki-notebook").load("https://2014.igem.org/Team:Tec-Monterrey/ITESM14_notebook.html?action=raw&amp;ctype=text/html");
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-  <script>
-    $(function(){
-      $("#wiki-protocols").load("https://2014.igem.org/Team:Tec-Monterrey/ITESM14_protocols.html?action=raw&amp;ctype=text/html");
-    });
-  </script>
-<div id="wiki-body">
+<h3>Week of September 26th</h3>
+<h5>- PCR Purification of V3a</h5>
+<h5>- Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94</h5>
+<h5>- PCR Check GG73-74 and GG75-82</h5>
+<h5>- Dilution V3a</h5>
+<h5>- Run Gel V3a</h5>
+<h5>- Transformation of GG61-64 (done twice) and GG65-72 and GG73-74</h5>
+<h5>- TX-TL re-run for W & M plasmids</h5>
+<h5>- Colony PCR GG61-64 and GG65-72</h5>
+<img src="https://static.igem.org/mediawiki/2016/c/c6/T--Alverno_CA--Screenshot_2016-10-15_09.09.08.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- LB Chloramphenicol plates & LB Kanamycin plates</h5>
+<img src="https://static.igem.org/mediawiki/2016/6/65/T--Alverno_CA--Screenshot_2016-10-15_09.09.17.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Wiki Work</h5>
-<body>
+<h3>Week of October 3rd</h3>
+<img src="https://static.igem.org/mediawiki/2016/5/50/T--Alverno_CA--Screenshot_2016-10-15_09.09.24.png" alt="iGEM" style="width:20%; height:20%">
-  <header id="wiki-header" class="initial-nav-scroll-up">
+<h5>- Middle School Visit</h5>
-  </header>
+<h5>- LB Broth made</h5>
+<h5>- PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab</h5>
-<div id ="wiki-content" class="main-content">
+<h5>- Dilution V3a</h5>
+<h5>- PCR Check GG83-90</h5>
-  <div class="jumbotron" style="background-color:transparent; padding-top: 0; margin-bottom: 0; padding-bottom: 0; padding-left:0;">
+<h5>- PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab</h5>
-    <div class="container">
+<h5>- TX-TL re-run for W & M plasmids w/ Replicates</h5>
-      <div id="overviewheader" style="display: none;">
+<h5>- Gels Made</h5>
-        <img class="col-sm-6 col-sm-offset-3  img img-responsive" style="padding:0px 0px 0px 0px" src="https://static.igem.org/mediawiki/2014/6/6c/ITESM14_Projectheader.png">
+<h5>- Mini prep GG28 1-5</h5>
-        <h1 class="col-sm-8 col-sm-offset-2 text-center" style="color: #f4ff3b; font-size: 8vw; font-weight: lighter; margin-top:0; margin-bottom:20px; padding:0;">Overview</h1>
+<h5>- Make TBE 1x</h5>
-      </div>
+<img src="https://static.igem.org/mediawiki/2016/9/99/T--Alverno_CA--Screenshot_2016-10-15_09.09.30.png" alt="iGEM" style="width:20%; height:20%">
-      <div id="notebookheader" style="display: none;">
+<h5>- Gel Run P1a-P4a, T1a-T4a, P1ab, P2ab, T3ab, T4ab & GG95-102</h5>
-        <img class="col-sm-6 col-sm-offset-3 img img-responsive" style="padding:0px 0px 0px 0px" src="https://static.igem.org/mediawiki/2014/0/05/ITESM14_NotebookHeader.png">
+<h5>- Primers Diluted</h5>
-        <h1 class="col-sm-8 col-sm-offset-2 text-center" style="color: #f4ff3b; font-size: 8vw; font-weight: lighter; margin-top:0; margin-bottom:20px; padding:0;">Notebook</h1>
+<h5>- Golden Gate GG103 & GG104 & GG85-102 & GG105-112</h5>
-      </div>
+<h5>- Transformation GG95-102 (done twice)</h5>
-      <div id="protocolsheader" style="display: none;">
+<h5>- Testing Fluorescence on GG28 1-5 w/ Plate Reader</h5>
-        <img class="col-sm-6 col-sm-offset-3  img img-responsive" style="padding:0px 0px 0px 0px" src="https://static.igem.org/mediawiki/2014/3/32/ITESM14_Protocolsheader.png">
+<h5>- Wiki Work</h5>
-        <h1 class="col-sm-8 col-sm-offset-2 text-center" style="color: #f4ff3b; font-size: 8vw; font-weight: lighter; margin-top:0; margin-bottom:20px; padding:0;">Protocols</h1>
-      </div>
-      <div id="modelingheader" style="display: none;">
-        <img class="col-sm-6 col-sm-offset-3  img img-responsive" style="padding:0px 0px 0px 0px" src="https://static.igem.org/mediawiki/2014/6/6a/ITESM14_Modelingheader.png">
-        <h1 class="col-sm-8 col-sm-offset-2 text-center" style="color: #f4ff3b; font-size: 7vw; font-weight: lighter; margin-top:0; margin-bottom:20px; padding:0;">Mathematical Models</h1>
-      </div>
-      <div id="module1header" style="display: none;">
-        <img class="col-sm-6 col-sm-offset-3  img img-responsive" style="padding:0px 0px 0px 0px" src="https://static.igem.org/mediawiki/2014/4/48/ITESM14_Module1header.png">
-        <h1 class="col-sm-8 col-sm-offset-2 text-center" style="color: #f4ff3b; font-size: 5vw; font-weight: lighter; margin-top:0; margin-bottom:20px; padding:0;">Module 1: Attenuation</h1>
-      </div>
-      <div id="module2header" style="display: none;">
-        <img class="col-md-6 col-sm-offset-3  img img-responsive" style="padding:0px 0px 0px 0px;" src="https://static.igem.org/mediawiki/2014/a/ab/ITESM14_Module2header.png">
-        <h1 class="col-sm-8 col-sm-offset-2 text-center" style="color: #f4ff3b; font-size: 5vw; font-weight: lighter; margin-top:0; margin-bottom:20px; padding:0;">Module 2: Phage engineering</h1>
-      </div>
-      <div id="module3header" style="display: none;">
-        <img class="col-sm-6 col-sm-offset-3  img img-responsive" style="padding:0px 0px 0px 0px;" src="https://static.igem.org/mediawiki/2014/6/66/ITESM14_module3header.png">
-        <h1 class="col-sm-8 col-sm-offset-2 text-center" style="color: #f4ff3b; font-size: 5vw; font-weight: lighter; margin-top:0; margin-bottom:20px; padding:0;">Module 3: Quorum sensing</h1>
-      </div>
-      <div id="module4header" style="display: none;">
-        <img class="col-sm-6 col-sm-offset-3  img img-responsive" style="padding:0px 0px 0px 0px" src="https://static.igem.org/mediawiki/2014/2/21/ITESM14_module4header.png">
-        <h1 class="col-sm-8 col-sm-offset-2 text-center" style="color: #f4ff3b; font-size: 6vw; font-weight: lighter; margin-top:0; margin-bottom:20px; padding:0;">Module 4: Effectors</h1>
-      </div>
-    </div>
-  </div>
-  <div class="well">
-  <div class="container">
-    <!--===============================Tab navigation======================================-->
-    <ul class="nav nav-tabs nav-justified" role="tablist" id="myTab" style="font-family: 'Yanone Kaffeesatz';">
-      <li class="active"><a id="overviewTab" href="#overview" role="tab" data-toggle="tab">Overview</a></li>
-      <li><a id="notebookTab" href="#notebook" role="tab" data-toggle="tab">Notebook</a></li>
-      <li><a id="protocolsTab" href="#protocols" role="tab" data-toggle="tab">Protocols</a></li>
-      <li><a id="modelingTab" href="#modeling" role="tab" data-toggle="tab">Modeling</a></li>
-      <li><a id="module1Tab" href="#module1" role="tab" data-toggle="tab">Module I</a></li>
-      <li><a id="module2Tab" href="#module2" role="tab" data-toggle="tab">Module II</a></li>
-      <li><a id="module3Tab" href="#module3" role="tab" data-toggle="tab">Module III</a></li>
-      <li><a id="module4Tab" href="#module4" role="tab" data-toggle="tab">Module IV</a></li>
-    </ul>
-    <!--===============================Tab content==================================================-->
-    <div class="tab-content">
-      <div class="tab-pane active" id="overview">
-        <div style="margin-top:2%; font-size:2vw;">
-          <p>
-            Over the past decades, Mexico has undergone a series transformations. Wealth has increased, families are having fewer children, and there has been improved access to health care for the poor. However, its public health resources now face a growing burden from a familiar challenge: cancer, which has become the second cause of mortality.
-          </p>
-          <p>
-            Dr. Alejandro Mohar, Director General of the National Cancer Institute of Mexico (INCan) noted that cancer is currently the second leading cause of death in the country, with approximately 125,000 new cases each year. The latest projection is that left uncontrolled, this rate will double to 250,000 new cancer cases per year by 2030. Thus, our project aims to contribute to the various novel therapies that are being developed using a more specific method to deliver a therapy.
-          </p>
-          <p>
-            By harnessing the inherent ability of facultative anaerobic bacteria to colonize and grow in tumoral environments, this project aims to develop a bacterial cancer therapy. A genetically modified E. coli strain will have knockouts in the lpp and msbB genes, which encode for the Braun's lipoprotein and for the myristic acid moiety transporter of the lipopolysaccharide, respectively. These genes are known to trigger an immune response in the human body; by deleting them, the impact originating from a bacterial intravenous administration will be reduced. Furthermore, these bacteria will produce M13-modified bacteriophages under the control of a quorum sensing system. The resulting phages will be capable of binding to the GRP78 receptor, which is usually overexpressed in cancerous cells. Subsequently, they will internalize and transfect the cancerous cells with two different genes: apoptin, responsible for an apoptotic protein specific for cancerous cells which will be regulated by a constitutive promoter and a survivin siRNA. The latter will inhibit the uncontrollable growth characteristic of cancer cells, making them more vulnerable to apoptosis.
-          </p>
-        </div>
-        <hr>
-        <h1>References</h1>
-          <ol>
-          <li><p>
-          Schatzkin, E. (2012, February 21). Cancer: Mexico’s Growing Problem. Retrieved September 14, 2014, from <a href="http://globalhealthsciences.ucsf.edu/news-events/cancer-mexico-s-growing-problem" target="_blank">http://globalhealthsciences.ucsf.edu/news-events/cancer-mexico-s-growing-problem</a>
-          </p></li>
-      </div>
-      <div class="tab-pane" id="notebook">
-          <div id="wiki-notebook"></div>
-      </div><!--end notebook-->
-      <div class="tab-pane" id="protocols">
-          <div id="wiki-protocols"></div>
-      </div><!--end protocols-->
-      <div class="tab-pane" id="modeling">
-         <div id="wiki-modeling"></div>
-      </div><!--end modeling-->
-      <div class="tab-pane" id="module1">
-          <div id="wiki-module1"></div>
-      </div><!--end module1-->
-      <div class="tab-pane" id="module2">
-          <div id="wiki-module2"></div>
-      </div><!--end module2-->
-      <div class="tab-pane" id="module3">
-          <div id="wiki-module3"></div>
-      </div><!--end module3-->
-      <div class="tab-pane" id="module4">
-          <div id="wiki-module4"></div>
-      </div><!--end module4-->
-    </div> <!-- tab-content -->
-  </div><!-- container -->
-  </div><!-- well -->
+<h3>Week of October 10th</h3>
+<h5>- Golden Gate GG113-114</h5>
+<h5>- Transformation GG103 & 104, GG105-112</h5>
+<h5>- LB Chloramphenicol plates made</h5>
+<h5>- PCR Purification of UC13a-UC15a</h5>
+<h5>- Transformation done GG113-114</h5>
+<h5>- LB Broth Made</h5>
+<h5>- Mini Prep GG95 (1)-GG102 (3)</h5>
+<h5>- Dilute Primers</h5>
+<h5>- Test Fluorescence of GG95-102_1-3 & GG105-108_1-4 (by TX-TL)</h5>
+<h5>- Purification of Plasmid DNA GG95-102_1-3 & GG105-108_1-4</h5>
+<h3>Week of October 17th</h3>
+<h5>- WIKI WORK!!!</h5>
 </body>
-<footer id="wiki-footer" >
-</footer>
 <script>
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