Difference between revisions of "Team:Alverno CA/Notebook"

Latest revision as of 03:49, 20 October 2016

Alverno iGEM 2016

Notebook

Week of June 6th (Limited amount of notes taken)

- Lots of Designing Parts and Primers

- Wiki Work

Week of June 13th:

- Cleaning Restriction Digest and Ligation Done

- Ampicillin plates made

- Ampicillin stock made

- Transform RFP plasmid

- Design Parts and Primers

- Wiki Work

Week of June 20th

- Transformation Done (probably GFP)

- RFP and GRP colonies

- PCR for RFP Coding Gene

- Design Parts and Primers

- Wiki Work

Week of June 27th

- PCR Done for promoters, terminators, some UC parts

- Primer Dilutions Done

- Gel Run with PCR product from Week of June 20th (RFP)

- Miniprep kit for RFP Bba

- TBE Buffer 10x and 1x made

- PCR Purification done on Promoters and Terminators

- Wiki Work

Week of July 4th

- PCR of Vectors, some UC parts

- Primer Dilutions

- Gel Run UC16-18 and UC2 (UC18 ran twice)

- PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)

- Plate Reader

- Wiki Work

Week of July 11th

- Learn how to use Benchling

- Primers Diluted

- Parts Diluted

- PCR done on P1ab, P2ab, T3ab, T4ab

- Gel Run on P1ab, P2ab, T3ab, T4ab

- PCR Clean up done on P1ab, P2ab, T3ab, T4ab

- Chloramphenicol plates made

- Wiki Work

Week of July 18th

- UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)

- Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d

- Transformation Done

- Chloramphenicol plates made

- Parts dilutions Done

- PCR of GG Assemblies

- PCR of UC3a-UC9a

- Wiki Work

Week of July 25th

- Set up Plate reader

- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts

- PCR Purification of UC3-9, V1a & V2a

- Picked Colonies

- Transformation Protocol for GG1-8

- Gel made & Gel made w/ SYBR safe

- PCR of GG Assemblies

- PCR for V1a and V2a, UC7-9

- Dilutions Done

- Gel Run for UC7-9

- Golden Gate Assembly GG9-16

- Wiki Work

Week of August 1st

- Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23

- PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12

- PCR Purification for UC7-8

- Parts Dilutions done UC2 & UC3-6 & UC7-8

- PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23

- Fixed Thermocycler

- Gels Made

- Agar Plates made w/ Kan

- Golden Gate Assembly GG17-19 & GG20-23 & GG24-26

- Transformation with GG12

- Ludox Calibration

- Test Fluorescence of Plasmids

- Wiki Work

Week of August 8th

- Cell Measurement

- Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR

- PCR Check for GG24-26 & GG27-28

- Made Gel

- Transformation with GG24-26 & GG27-28

- Golden Gate Assembly GG27-28

- Colony PCR Check of GG24-26

- Primer Dilutions

- Plasmid DNA Purification

- LB Kan Broth

- Wiki Work

Week of August 15th

- Lab Meet-up with UCL/LC/CV

- Colony PCR Check for GG libraries 27-28

- Gels Made

- Gel Run for GG Library 28

- Grow Cultures GG27

- Learn Python

- Check Fluorescence for GG28 & GG27

- PCR UC14a

- Purification of Plasmid DNA

- Wiki Work

Week of August 22nd

- Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36

- Gels Made

- PCR UC13a and UC15a (Done twice) & UC1

- PCR Purification of UC14 & UC13/15 & UC1

- Golden Gate Assembly for GG29-36

- PCR Check for GG29-36

- LB Kan Broth Made

- Dilution of UC1

- Control run in Plate Reader

- Wiki Work

Week of August 29th

- Make Gels

- Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a

- Golden Gate Assembly GG37-52 & GG53-60

- PCR Check GG37-52 & GG53-60

- PCR Check Gel run for GG37-52

- PCR CDS1a & CDS2a

- PCR Purification of CDS1a & CDS2a

- Talk with iGEM Peshawar

- LB Kan Plates Made

- Wiki Work

Week of September 5th

- iGEM Team Meeting

- PCR Purification of CDS1a & CDS2a and UC1

- Transformation was done with GG53-60

- Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64

- Gels made

- Dilution of CDS1a & CDS2a

- Golden Gate Assembly GG61-64

- PCR of UC1

- LB Kan Plates Made

- PCR Check for GG61-64

- TBE 10x Buffer

- Wiki Work

Week of September 12th

- Talked with UCL iGEM Team

- LV-LC-CV meet-up

- Google Hangouts with Lambert Georgia iGEM team

- Dilution of UC1a

- Transformation of GG29-36

- Golden Gate Assembly GG65-72

- PCR GG61-64

- PCR Check GG61-64

- TX-TL of GFP (+ control)

- Outreach workshop with Middle school

- Wiki Work

Week of September 19th

- REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE

- Run Gel for GG61-64 (done twice) and GG65-72

- LB Kan Plates Made

- Gels Made

- TX-TL GFP Re-run

- Gel Run for UC1 and GG61-64

- Control Test ran using only the ladder for Gels

- TX-TL W & M plasmids

- PCR U3a

- Dilution of Primers

- Wiki Work

Week of September 26th

- PCR Purification of V3a

- Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94

- PCR Check GG73-74 and GG75-82

- Dilution V3a

- Run Gel V3a

- Transformation of GG61-64 (done twice) and GG65-72 and GG73-74

- TX-TL re-run for W & M plasmids

- Colony PCR GG61-64 and GG65-72

- LB Chloramphenicol plates & LB Kanamycin plates

- Wiki Work

Week of October 3rd

- Middle School Visit

- LB Broth made

- PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab

- Dilution V3a

- PCR Check GG83-90

- PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab

- TX-TL re-run for W & M plasmids w/ Replicates

- Gels Made

- Mini prep GG28 1-5

- Make TBE 1x

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-<head>
+ <head>
 <title>Alverno iGEM 2016</title>
-  <link rel="icon" href="https://pbs.twimg.com/profile_images/2199338457/alverno-logo_high_400x400.PNG">
+  <link rel="icon" href="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png">
 </head>
 <body>
-<h1><center>Alverno iGEM 2016</center></h1>
+<br>
-<center><img src="https://s-media-cache-ak0.pinimg.com/originals/86/08/18/860818cfc65e5ff04725bb0f0c05a8af.png" alt="Alverno iGEM Logo" style="width:300px;"</center>
+<br>
-<h1><center>Notebook</center></h1>
+<h2><center>Notebook</center></h2>
-<h3><b>Week of June 6th (Limited amount of notes taken):</b></h3>
+<center>
-<li>Lots of Designing Parts and Primers</li>
+<img class="icons"src="https://static.igem.org/mediawiki/2016/a/ac/T--Alverno_CA--labnotbook.jpg" style= width:500px>
-<li>Wiki Work</li>
+<center>
-<h3><b>Week of June 13th:</b></h3>
+<h3>Week of June 6th (Limited amount of notes taken)</h3>
-<li>Cleaning Restriction Digest and Ligation Done</li>
+<h5>- Lots of Designing Parts and Primers</h5>
-<li>Ampicillin plates made</li>
+<h5>- Wiki Work</h5>
-<li>Ampicillin stock made</li>
-<li>Transform RFP plasmid</li>
-<li>Design Parts and Primers</li>
-<li>Wiki Work</li>
-<h3><b>Week of June 20th</b></h3>
+<h3>Week of June 13th:</h3>
-<li>Transformation Done (probably GFP)</li>
+<h5>- Cleaning Restriction Digest and Ligation Done</h5>
-<li>RFP and GRP colonies</li>
+<h5>- Ampicillin plates made</h5>
-<li>PCR for RFP Coding Gene</li>
+<h5>- Ampicillin stock made</h5>
-<li>Design Parts and Primers</li>
+<h5>- Transform RFP plasmid</h5>
-<li>Wiki Work</li>
+<h5>- Design Parts and Primers</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of June 27th:</b></h3>
+<h3>Week of June 20th</h3>
-<li></li>
+<h5>- Transformation Done (probably GFP)</h5>
+<h5>- RFP and GRP colonies</h5>
+<h5>- PCR for RFP Coding Gene</h5>
+<h5>- Design Parts and Primers</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of July 4th:</b></h3>
+<h3>Week of June 27th</h3>
-<li></li>
+<h5>- PCR Done for promoters, terminators, some UC parts</h5>
+<h5>- Primer Dilutions Done</h5>
+<h5>- Gel Run with PCR product from Week of June 20th (RFP)</h5>
+<h5>- Miniprep kit for RFP Bba</h5>
+<h5>- TBE Buffer 10x and 1x made</h5>
+<h5>- PCR Purification done on Promoters and Terminators</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of July 11th:</b></h3>
+<h3>Week of July 4th</h3>
-<li></li>
+<h5>- PCR of Vectors, some UC parts</h5>
+<h5>- Primer Dilutions</h5>
+<h5>- Gel Run UC16-18 and UC2 (UC18 ran twice)</h5>
+<h5>- PCR Clean Up done for UC16-18, vectors, T3-T4 (terminators), UC2 (UC18 done twice)</h5>
+<h5>- Plate Reader</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of July 18th:</b></h3>
+<h3>Week of July 11th</h3>
-<li></li>
+<img src="https://static.igem.org/mediawiki/2016/4/44/T--Alverno_CA--Screenshot_2016-10-15_09.24.58.png" alt="iGEM" style="max-width:100%; max-height:100%">
+<h5>- Learn how to use Benchling</h5>
+<h5>- Primers Diluted</h5>
+<img src="https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.05.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Parts Diluted</h5>
+<h5>- PCR done on P1ab, P2ab, T3ab, T4ab</h5>
+<h5>- Gel Run on P1ab, P2ab, T3ab, T4ab</h5>
+<h5>- PCR Clean up done on P1ab, P2ab, T3ab, T4ab</h5>
+<h5>- Chloramphenicol plates made</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of July 25th:</b></h3>
+<h3>Week of July 18th</h3>
-<li></li>
+<h5>- UCLA iGEM meetup (UCLA, ULV, UCSD, and Marburg Teams)</h5>
+<img src="https://static.igem.org/mediawiki/2016/d/d5/T--Alverno_CA--Screenshot_2016-10-15_09.05.53.png" alt="iGEM" style="width:30%; height:30%">
+<h5>- Golden Gate Assembly Done with P1ab, P2ab, P3, P4, T1, T2, T3ab, T4ab, UC2, V2d</h5>
+<img src="https://static.igem.org/mediawiki/2016/5/5d/T--Alverno_CA--Screenshot_2016-10-15_09.06.03.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Transformation Done</h5>
+<h5>- Chloramphenicol plates made</h5>
+<h5>- Parts dilutions Done</h5>
+<h5>- PCR of GG Assemblies</h5>
+<h5>- PCR of UC3a-UC9a</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of August 1st:</b></h3>
+<h3>Week of July 25th</h3>
-<li></li>
+<img src="https://static.igem.org/mediawiki/2016/4/4f/T--Alverno_CA--Screenshot_2016-10-15_09.06.12.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Set up Plate reader</h5>
+<h5>- Run Gel UC3a-UC9a & GG1-8, V1a & V2a, GG Parts</h5>
+<h5>- PCR Purification of UC3-9, V1a & V2a</h5>
+<img src="https://static.igem.org/mediawiki/2016/d/d9/T--Alverno_CA--Screenshot_2016-10-15_09.06.21.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Picked Colonies</h5>
+<h5>- Transformation Protocol for GG1-8</h5>
+<h5>- Gel made & Gel made w/ SYBR safe</h5>
+<h5>- PCR of GG Assemblies</h5>
+<h5>- PCR for V1a and V2a, UC7-9</h5>
+<h5>- Dilutions Done</h5>
+<h5>- Gel Run for UC7-9</h5>
+<h5>- Golden Gate Assembly GG9-16</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of August 8th:</b></h3>
+<h3>Week of August 1st</h3>
-<li></li>
+<h5>- Run Gel for UC7-9 & (GG9-16 Done twice) & GG17-19 & GG20-23</h5>
+<h5>- PCR of GFP & RFP w/ varying UTRs & RFP chlor. GFP chlor. Kan GG12</h5>
+<h5>- PCR Purification for UC7-8</h5>
+<h5>- Parts Dilutions done UC2 & UC3-6 & UC7-8</h5>
+<h5>- PCR Check for GG9-16 (Done twice) & GG17-19 & GG20-23</h5>
+<img src="https://static.igem.org/mediawiki/2016/d/d6/T--Alverno_CA--Screenshot_2016-10-15_09.06.31.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Fixed  Thermocycler</h5>
+<h5>- Gels Made</h5>
+<h5>- Agar Plates made w/ Kan</h5>
+<h5>- Golden Gate Assembly GG17-19 & GG20-23 & GG24-26</h5>
+<img src="https://static.igem.org/mediawiki/2016/c/c2/T--Alverno_CA--Screenshot_2016-10-15_09.06.45.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Transformation with GG12</h5>
+<h5>- Ludox Calibration</h5>
+<h5>- Test Fluorescence of Plasmids</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of August 15th:</b></h3>
+<h3>Week of August 8th</h3>
-<li></li>
+<img src="https://static.igem.org/mediawiki/2016/5/53/T--Alverno_CA--Screenshot_2016-10-15_09.06.52.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Cell Measurement</h5>
+<img src="https://static.igem.org/mediawiki/2016/0/0e/T--Alverno_CA--Screenshot_2016-10-15_09.07.03.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Run Gel for GG24-26 & 24A-E, 25A-E, 26A-E, 27-28 PCR</h5>
+<h5>- PCR Check for GG24-26 & GG27-28</h5>
+<h5>- Made Gel</h5>
+<h5>- Transformation with GG24-26 & GG27-28</h5>
+<h5>- Golden Gate Assembly GG27-28</h5>
+<h5>- Colony PCR Check of GG24-26</h5>
+<h5>- Primer Dilutions</h5>
+<h5>- Plasmid DNA Purification</h5>
+<h5>- LB Kan Broth</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of August 22nd:</b></h3>
+<h3>Week of August 15th</h3>
-<li></li>
+<img src="https://static.igem.org/mediawiki/2016/5/5c/T--Alverno_CA--Screenshot_2016-10-15_09.07.17.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Lab Meet-up with UCL/LC/CV</h5>
+<h5>- Colony PCR Check for GG libraries 27-28</h5>
+<h5>- Gels Made</h5>
+<h5>- Gel Run for GG Library 28</h5>
+<h5>- Grow Cultures GG27</h5>
+<img src="https://static.igem.org/mediawiki/2016/0/0c/T--Alverno_CA--Screenshot_2016-10-15_09.07.26.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Learn Python</h5>
+<h5>- Check Fluorescence for GG28 & GG27</h5>
+<h5>- PCR UC14a</h5>
+<h5>- Purification of Plasmid DNA</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of August 29th:</b></h3>
+<h3>Week of August 22nd</h3>
-<li></li>
+<img src="https://static.igem.org/mediawiki/2016/a/ad/T--Alverno_CA--Screenshot_2016-10-15_09.07.34.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Gel Run for GG27 & UC13-15 UC13-15 and UC1 & GG29-36</h5>
+<h5>- Gels Made</h5>
+<h5>- PCR UC13a and UC15a (Done twice) & UC1</h5>
+<h5>- PCR Purification of UC14 & UC13/15 & UC1</h5>
+<h5>- Golden Gate Assembly for GG29-36</h5>
+<h5>- PCR Check for GG29-36</h5>
+<h5>- LB Kan Broth Made</h5>
+<h5>- Dilution of UC1</h5>
+<h5>- Control run in Plate Reader</h5>
+<img src="https://static.igem.org/mediawiki/2016/6/69/T--Alverno_CA--Screenshot_2016-10-15_09.07.46.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Wiki Work</h5>
-<h3><b>Week of September 5th:</b></h3>
+<h3>Week of August 29th</h3>
-<li></li>
+<img src="https://static.igem.org/mediawiki/2016/9/9b/T--Alverno_CA--Screenshot_2016-10-15_09.07.54.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Make Gels</h5>
+<h5>- Run Gel for UC13-UC15 & GG29-36 and CDS1a & CDS2a</h5>
+<h5>- Golden Gate Assembly GG37-52 & GG53-60</h5>
+<h5>- PCR Check GG37-52 & GG53-60</h5>
+<h5>- PCR Check Gel run for GG37-52</h5>
+<h5>- PCR CDS1a & CDS2a</h5>
+<h5>- PCR Purification of CDS1a & CDS2a</h5>
+<img src="https://static.igem.org/mediawiki/2016/d/d7/T--Alverno_CA--Screenshot_2016-10-15_09.08.02.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Talk with iGEM Peshawar</h5>
+<h5>- LB Kan Plates Made</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of September 12th:</b></h3>
+<h3>Week of September 5th</h3>
-<li></li>
+<img src="https://static.igem.org/mediawiki/2016/a/ae/T--Alverno_CA--Screenshot_2016-10-15_09.08.11.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- iGEM Team Meeting</h5>
+<h5>- PCR Purification of CDS1a & CDS2a and UC1</h5>
+<h5>- Transformation was done with GG53-60</h5>
+<h5>- Gel Run for CDS1 & CDS2 and UC1 (done 3 times) and GG61-64</h5>
+<h5>- Gels made</h5>
+<h5>- Dilution of CDS1a & CDS2a</h5>
+<h5>- Golden Gate Assembly GG61-64</h5>
+<h5>- PCR of UC1</h5>
+<h5>- LB Kan Plates Made</h5>
+<h5>- PCR Check for GG61-64</h5>
+<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.20.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- TBE 10x Buffer</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of September 19th:</b></h3>
+<h3>Week of September 12th</h3>
-<li></li>
+<img src="https://static.igem.org/mediawiki/2016/f/f2/T--Alverno_CA--Screenshot_2016-10-15_09.08.27.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Talked with UCL iGEM Team</h5>
+<h5>- LV-LC-CV meet-up</h5>
+<h5>- Google Hangouts with Lambert Georgia iGEM team</h5>
+<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Alverno_CA--Screenshot_2016-10-15_09.08.35.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Dilution of UC1a</h5>
+<h5>- Transformation of GG29-36</h5>
+<h5>- Golden Gate Assembly GG65-72</h5>
+<h5>- PCR GG61-64</h5>
+<h5>- PCR Check GG61-64</h5>
+<h5>- TX-TL of GFP (+ control)</h5>
+<img src="https://static.igem.org/mediawiki/2016/0/04/T--Alverno_CA--Screenshot_2016-10-15_09.08.42.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Outreach workshop with Middle school</h5>
+<h5>- Wiki Work</h5>
-<h3><b>Week of September 26th:</b></h3>
+<h3>Week of September 19th</h3>
-<li></li>
+<h5>- REVIEW OF EVERYTHING THAT NEEDS TO BE DONE BEFORE GOING TO JAMBOREE</h5>
+<img src="https://static.igem.org/mediawiki/2016/4/4a/T--Alverno_CA--Screenshot_2016-10-15_09.08.50.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Run Gel for GG61-64 (done twice) and GG65-72</h5>
+<h5>- LB Kan Plates Made</h5>
+<h5>- Gels Made</h5>
+<h5>- TX-TL GFP Re-run</h5>
+<h5>- Gel Run for UC1 and GG61-64</h5>
+<h5>- Control Test ran using only the ladder for Gels</h5>
+<h5>- TX-TL W & M plasmids</h5>
+<h5>- PCR U3a</h5>
+<h5>- Dilution of Primers</h5>
+<img src="https://static.igem.org/mediawiki/2016/3/3f/T--Alverno_CA--Screenshot_2016-10-15_09.09.01.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Wiki Work</h5>
-<h3><b>Week of October 3rd:</b></h3>
+<h3>Week of September 26th</h3>
-<li></li>
+<h5>- PCR Purification of V3a</h5>
+<h5>- Golden Gate Assembly GG73-74 and GG75-82 and GG83-90 and GG91-94</h5>
+<h5>- PCR Check GG73-74 and GG75-82</h5>
+<h5>- Dilution V3a</h5>
+<h5>- Run Gel V3a</h5>
+<h5>- Transformation of GG61-64 (done twice) and GG65-72 and GG73-74</h5>
+<h5>- TX-TL re-run for W & M plasmids</h5>
+<h5>- Colony PCR GG61-64 and GG65-72</h5>
+<img src="https://static.igem.org/mediawiki/2016/c/c6/T--Alverno_CA--Screenshot_2016-10-15_09.09.08.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- LB Chloramphenicol plates & LB Kanamycin plates</h5>
+<img src="https://static.igem.org/mediawiki/2016/6/65/T--Alverno_CA--Screenshot_2016-10-15_09.09.17.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Wiki Work</h5>
-<h3><b></b></h3>
+<h3>Week of October 3rd</h3>
+<img src="https://static.igem.org/mediawiki/2016/5/50/T--Alverno_CA--Screenshot_2016-10-15_09.09.24.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Middle School Visit</h5>
+<h5>- LB Broth made</h5>
+<h5>- PCR P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab</h5>
+<h5>- Dilution V3a</h5>
+<h5>- PCR Check GG83-90</h5>
+<h5>- PCR Purification P1a-P4a & T1a-T4a & P1ab &P2ab & T3ab & T4ab</h5>
+<h5>- TX-TL re-run for W & M plasmids w/ Replicates</h5>
+<h5>- Gels Made</h5>
+<h5>- Mini prep GG28 1-5</h5>
+<h5>- Make TBE 1x</h5>
+<img src="https://static.igem.org/mediawiki/2016/9/99/T--Alverno_CA--Screenshot_2016-10-15_09.09.30.png" alt="iGEM" style="width:20%; height:20%">
+<h5>- Gel Run P1a-P4a, T1a-T4a, P1ab, P2ab, T3ab, T4ab & GG95-102</h5>
+<h5>- Primers Diluted</h5>
+<h5>- Golden Gate GG103 & GG104 & GG85-102 & GG105-112</h5>
+<h5>- Transformation GG95-102 (done twice)</h5>
+<h5>- Testing Fluorescence on GG28 1-5 w/ Plate Reader</h5>
+<h5>- Wiki Work</h5>
-<h3><b></b></h3>
+<h3>Week of October 10th</h3>
+<h5>- Golden Gate GG113-114</h5>
+<h5>- Transformation GG103 & 104, GG105-112</h5>
+<h5>- LB Chloramphenicol plates made</h5>
+<h5>- PCR Purification of UC13a-UC15a</h5>
+<h5>- Transformation done GG113-114</h5>
+<h5>- LB Broth Made</h5>
+<h5>- Mini Prep GG95 (1)-GG102 (3)</h5>
+<h5>- Dilute Primers</h5>
+<h5>- Test Fluorescence of GG95-102_1-3 & GG105-108_1-4 (by TX-TL)</h5>
+<h5>- Purification of Plasmid DNA GG95-102_1-3 & GG105-108_1-4</h5>
-<h3><b></b></h3>
+<h3>Week of October 17th</h3>
+<h5>- WIKI WORK!!!</h5>
+</body>
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