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| <p>In view of existing as well as potential future uranium pollution, the current complicated, time-consuming, inefficient and expensive treatment methods seem woefully out-of-date. To address these drawbacks, the Peking iGEM team put forward the Uranyl Reaper project, which aimed to develop an innovative method to deal with uranium pollution.</p> | | <p>In view of existing as well as potential future uranium pollution, the current complicated, time-consuming, inefficient and expensive treatment methods seem woefully out-of-date. To address these drawbacks, the Peking iGEM team put forward the Uranyl Reaper project, which aimed to develop an innovative method to deal with uranium pollution.</p> |
| <p>In order to achieve this goal, we designed an extraordinary biological adsorption polymer network which was produced by engineered bacteria and self-assembled completely autonomously. Based on the isopeptide bond formed between SpyCatcher and SpyTag, two designed crosslinking peptides, we developed triple SpyTag and SpyCatcher modules which could form a covalently-linked polymer network which offers high mechanical strength and high stability in diverse challenging environments.</p> | | <p>In order to achieve this goal, we designed an extraordinary biological adsorption polymer network which was produced by engineered bacteria and self-assembled completely autonomously. Based on the isopeptide bond formed between SpyCatcher and SpyTag, two designed crosslinking peptides, we developed triple SpyTag and SpyCatcher modules which could form a covalently-linked polymer network which offers high mechanical strength and high stability in diverse challenging environments.</p> |
− | <p>This polymer network further gains the capacity to adsorb uranium in aqueous environments with high efficiency via the addition of a super uranyl binding protein (SUP) module. The SUP could also be replaced with other functional elements such as additional metal binding proteins, or the monomerized streptavidin (mSA) module which could mediate the retrievement of the self-assembled polymer network through biotin-avidin interactions in order to achieve multi-functionality and modularization.</p> | + | <p>This polymer network further gains the capacity to adsorb uranium in aqueous environments with high efficiency via the addition of a super uranyl binding protein (SUP) module. The SUP could also be replaced with other functional elements such as additional metal binding proteins, or the monomerized streptavidin (mSA) module which could mediate the clearance of the self-assembled polymer network through biotin-avidin interactions in order to achieve multi-functionality and modularization.</p> |
| <p>We utilized a series of signal peptides to ensure efficient secretion. A schematic graph of the whole project is shown in Fig. 1. | | <p>We utilized a series of signal peptides to ensure efficient secretion. A schematic graph of the whole project is shown in Fig. 1. |
| <figure> | | <figure> |
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| <a id="Crosslinking"></a> | | <a id="Crosslinking"></a> |
− | <h3 class="classic-title anchor"><span>Local Concentration Enhancement - Crosslinking</span> </h3> | + | <h3 class="classic-title anchor"><a href="https://2016.igem.org/Team:Peking/Crosslinking"><span>Local Concentration Enhancement - Crosslinking</span> </a></h3> |
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| <a id="Uranyl"></a> | | <a id="Uranyl"></a> |
− | <h3 class="classic-title anchor"><span>Reaping the ions - Uranyl Adsorption</span></h3> | + | <h3 class="classic-title anchor"><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption"><span>Reaping the ions - Uranyl Adsorption</span></a></h3> |
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| <a id="Collection"></a> | | <a id="Collection"></a> |
− | <h3 class="classic-title anchor"><span>Retrieving the network - Clearance</span></h3> | + | <h3 class="classic-title anchor"><a href="https://2016.igem.org/Team:Peking/Clearance"><span>Retrieving the network - Clearance</span></a></h3> |
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| <a id="Secretion"></a> | | <a id="Secretion"></a> |
− | <h3 class="classic-title anchor"><span>Simplification of protein purification - Secretion</span></h3> | + | <h3 class="classic-title anchor"><a href="https://2016.igem.org/Team:Peking/Secretion"><span>Simplification of protein purification - Secretion</span></a></h3> |
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| <div style="width:100%"> | | <div style="width:100%"> |